Dorsal Root Ganglia (Mouse) Protein Lysate Preparation - Translational Pain Research Lab Protocols

In this video, lumbar dorsal root ganglia tissue (L3/L4) is lysed in RIPA buffer using a micro glass homogenizer.
This procedure was captured using a GoPro camera.

[@biogamer93]

Great vid. Can you also do that with IPH ? and in an eppendorf with a plastic homogenizer tip ? Also, what does the final volume of Lysate need to be ?