How do you know what broth/media to use for each bacteria? For example what broth would I use for salmonella or listeria? Where is the best place to find this information?
We tend to refer students to the "Oxoid manual". This is now part of Thermofisher: www.thermofisher.com/uk/en/home/industrial/microbiology.html If you use the search bar and filter the results for media you will find some ideas. You can also search for research papers (perhaps using Google Scholar) which describe methods for culturing the organism(s) of interest - they are likely to state the media used.
Hi sir gud evening I have an small doubt.... After completion of msc microbiology which kind of job is good for us.... Actually I don't know anything about microbiology but i have to take this group with the combination of microbiology and biochemistry..... In microbiology we have lot of choices in which of them which one is good
Questions like this as so hard to answer as one person's interests will be so different to someone else's. For some ideas about careers in microbiology have a look at the microbiology society's website here: microbiologysociety.org/careers.html I'm sure you will find something of interest!
When working aseptically gloves can be useful to minimise contamination. However, when teaching these techniques to inexperienced students the wider balance of risks are considered which includes the hazard of the Bunsen burner flame. At this level, working with low risk organisms, we make the judgement that it is better not to wear gloves due to the risk of burns due to the potential of the flame to melt the glove onto skin. These decisions take all this into account and if we were working with more harmful organisms we might insist on gloves.
Should we vortex the tube before inoculating ?
Thanks. I was a little lost
How do you know what broth/media to use for each bacteria? For example what broth would I use for salmonella or listeria? Where is the best place to find this information?
We tend to refer students to the "Oxoid manual". This is now part of Thermofisher:
www.thermofisher.com/uk/en/home/industrial/microbiology.html
If you use the search bar and filter the results for media you will find some ideas.
You can also search for research papers (perhaps using Google Scholar) which describe methods for culturing the organism(s) of interest - they are likely to state the media used.
Hi sir gud evening
I have an small doubt.... After completion of msc microbiology which kind of job is good for us.... Actually I don't know anything about microbiology but i have to take this group with the combination of microbiology and biochemistry..... In microbiology we have lot of choices in which of them which one is good
Questions like this as so hard to answer as one person's interests will be so different to someone else's. For some ideas about careers in microbiology have a look at the microbiology society's website here: microbiologysociety.org/careers.html I'm sure you will find something of interest!
Great video! much love. Keep it up guys! xxxx
how many days would it approximately take if it is incubated at room temperature?
This depends on the organism we are culturing. For a typical E. coli an overnight incubation at 37degC of up to 24h would be sufficient.
Where can I get a broth culture done?
These techniques are typically carried out in microbiology laboratories.
What type of media broth and agar used with E coli in this video?
We used nutrient broth and nutrient agar.
For the purposes of the video we used nutrient broth.
At what temperature was it incubated and how many days?
This depends on the organism we are culturing. For a typical E. coli an overnight incubation at 37degC of up to 24h would be sufficient.
Thanks a lots ❤️ do you have website?
Our website is www.lincoln.ac.uk/home/course/micbioms/
What is broth
Broth is a general term for the liquid medium we use to culture the organisms. In this case, we used a standard nutrient broth.
No gloves & No Hairnet
When working aseptically gloves can be useful to minimise contamination. However, when teaching these techniques to inexperienced students the wider balance of risks are considered which includes the hazard of the Bunsen burner flame. At this level, working with low risk organisms, we make the judgement that it is better not to wear gloves due to the risk of burns due to the potential of the flame to melt the glove onto skin. These decisions take all this into account and if we were working with more harmful organisms we might insist on gloves.
Hi Microsoft teams class