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In this particular video we used 0.1 mL or 100 uL, this is a fairly standard volume for spread plating. The most important thing is to know what volume was used so that if you are counting colonies later you can take it into account when working out how many viable cells (CFU/mL) were in your original sample. Good luck for the exam tomorrow!

Agree, when working aseptically gloves can be useful to minimise contamination. However, when teaching these techniques to inexperienced students the wider balance of risks are considered which includes the hazard of the Bunsen burner flame. At this level, working with low risk organisms, we make the judgement that it is better not to wear gloves due to the risk of burns due to the potential of the flame to melt the glove onto skin. These decisions take all this into account and if we were working with more harmful organisms we might insist on gloves.

When working aseptically gloves can be useful to minimise contamination. However, when teaching these techniques to inexperienced students the wider balance of risks are considered which includes the hazard of the Bunsen burner flame. At this level, working with low risk organisms, we make the judgement that it is better not to wear gloves due to the risk of burns due to the potential of the flame to melt the glove onto skin. These decisions take all this into account and if we were working with more harmful organisms we might insist on gloves.

Broth is a general term for the liquid medium we use to culture the organisms. In this case, we used a standard nutrient broth.

We consider the sample we are working with, what we are trying to achieve and who is carrying out the work when deciding whether to wear gloves. Gloves may be worn and there are times they are essential but in some circumstances, gloves could be considered more dangerous (particularly if worn by someone who is inexperienced) close to a Bunsen burner flame.

This can depend on the sample and what we are trying to achieve but typically this would be around 20 mL.

These techniques are typically carried out in microbiology laboratories.

We tend to refer students to the "Oxoid manual". This is now part of Thermofisher: www.thermofisher.com/uk/en/home/industrial/microbiology.html If you use the search bar and filter the results for media you will find some ideas. You can also search for research papers (perhaps using Google Scholar) which describe methods for culturing the organism(s) of interest - they are likely to state the media used.

A full risk assessment is always carried out before working in the laboratory and part of that determines whether gloves are worn or not. This depends mostly on the organism being cultured / procedure being carried out and on many occasions it is actually considered safer not to wear gloves because of the risks associated with working close to the Bunsen burner flame.

I think they probably all take around the same time. I guess it depends on how you work if this one is taking longer for you. Do you mean because you have to wait for the agar to set? In the other methods the agar has already been prepared and set so we don't need to worry about that time here.

There is likely to be less oxygen within the agar than there is in the air that the surface of the agar is exposed to. So it is probably better to say that obligate aerobes are more likely to grow on the surface of the agar than within the agar and that aerobes that need higher concentrations of oxygen to successfully grow would not be present within the agar if there is insufficient oxygen there for them. For anaerobes to grow, an anaerobic cabinet or maybe anaerobic jars would be required.

Questions like this as so hard to answer as one person's interests will be so different to someone else's. For some ideas about careers in microbiology have a look at the microbiology society's website here: microbiologysociety.org/careers.html I'm sure you will find something of interest!

When we use glassware we flame the neck of the bottles to remove any microorganisms that could potentially cause contamination. This is part of our aseptic technique. We would only do this with glassware though, not if we are using single use plastics.

We consider the sample we are working with, what we are trying to achieve and who is carrying out the work when deciding whether to wear gloves. Gloves may be worn and there are times they are essential but in some circumstances, gloves could be considered more dangerous (particularly if worn by someone who is inexperienced) close to a Bunsen burner flame.

We consider the sample we are working with, what we are trying to achieve and who is carrying out the work when deciding whether to wear gloves. Gloves may be worn and there are times they are essential but in some circumstances, gloves could be considered more dangerous (particularly if worn by someone who is inexperienced) close to a Bunsen burner flame.

i realize I am kind of randomly asking but does anyone know a good site to watch newly released movies online?

@Avery Iker Meh try Flixportal. just search on google after it:P -alejandro

@Alejandro Wesson thanks, signed up and it seems to work :D I really appreciate it!!

@Avery Iker glad I could help :D

This depends on the sample and what you are trying to achieve. Gloves may be worn but in some circumstances, gloves could be considered more dangerous (particularly if worn by someone who is inexperienced) close to a Bunsen burner flame. We suggest you refer to the specific guidance that will be provided in the laboratory you are working in and may depend on the organisms you are working with.

gloves for pussies ;)

This depends on the organism we are culturing. For a typical E. coli an overnight incubation at 37degC of up to 24h would be sufficient.

This depends on the organism we are culturing. For a typical E. coli an overnight incubation at 37degC of up to 24h would be sufficient.

Dear Maya, Compact Dry is a commercially available system for counting microorganisms. There are a number of different Compact Dry media products that you would need to take into account when interpreting your results. You can find some information about the range of products here: www.laensenadacorp.com/documentos/r-biopharm-COMPACT-DRY.pdf

Our website is www.lincoln.ac.uk/home/course/micbioms/

We used nutrient broth and nutrient agar.

For the purposes of the video we used nutrient broth.

mixed 1ml bacteria with agar

You could do this either way. The important thing is to ensure they are well-mixed.