THESE VIDEOS LITERALLY SAVE MY BIO GRADE LIKE I DONT UNDERSTAND THE WHOLE WEEK IN MY CLASS BUT BAM 8 MINUTES VIDEO AND I KNOW AND UNDERSTAND EVERYTHING THANK YOU
@@SixOhFivewhy not? there’s so many winners who during their careers expressed getting the Nobel prize was part of the achievements to conquer but even if the desire to get the noble was the ONLY reason why someone starts a career in motion it doesn’t take absolutely nothing away from the work done that took you there because is your work that speaks for the worthiness to the prize, not the timeline a person sets a goal for themselves. Btw @303404jb can I also get a shoutout when you get the noble prize because I’m defending you from unsupportive commenters ? Oh but after your mum, because mums are always first okay….
I didn't have an interpreter on the day I did this lab in my class, and I was so lost! I was nervous to find out that this process was going to be on exam! Yikes! However, I finally understand thank you! The closed caption helped me significantly, again thank you SO MUCH! :)
I'm really grateful to have found this...It's going to be my first year teaching AP bio, so your videos are very helpful, and answer many of my questions that I have from reading the manuals...
This video was awesome. I spent a week on this with my IB Bio 2 class and the easy diagrams really helped explain what I was doing in the labs. Thanks, I wish you were my professor!
Hope you guys pursue biology as a major/minor in college, because, in high school, you study the experiments and what happens, as well as what we now understand. But in college, you get to actually perform these experiments and reinforce what you studied in high school and relearned in college!
thank you for posting this. I get nervous about labs so I always try to find videos that show me how to do stuff because i get scared of all the stuff i have to do !!
1:20 . For some reason ,for plate 4 , almost 90% of the lawn glow when shined with UV light .. which contract because it is supposed to glow in clusters . So what went wrong ? Anyone know ??
Hello sir.....ph.d in molecular biology involves animal dissections(like where to extract DNA etc from)too as lam from zoology background....please reply
GFP is just a protein, but plasmids are DNA. DNA has to be transcribed into mRNA and then translated into a functional protein. The gene for GFP is useless if there is not a starting point for transcription called a "promoter"--that is the araC that you see in the video. AraC is an inducible promoter, meaning that it is usually off but the presence of some chemical (arabinose) can turn it on. Thus, arabinose will turn on the promoter, which initiates transcription of the GFP gene.
Beneficial is relative to the context of the selective pressures of the environment. A common transformation protocol involves the solution mentioned in the video alongside a heat shock, which will shock the bacteria and "trick" them into uptaking dna. What is not mentioned is that bacteria can get rid of useless plasmids during replication, which is why it is important that these plasmids for lab work have a selectable marker (such as one that encodes for resistance to an antibiotic).
The reason why divalent cations (basically always Ca++) work is because they can simultaneously be attracted the negatively charged bacterial membranes and the negatively charge DNa. This allows Ca++ to basically bring the DNA closer to the cell. Monovalent cations would only be able to be attracted to one or the other.
You need to learn how fun it is for your betters to beat up people like you on-line. You go ahead and tell me why a university with 50K student body wants to do a chemical poration when an electro-poration is so much more efficient. u need to tell me!
![I.N "HALLUCINATION" | [Stray Kids : SKZ-PLAYER]](http://i.ytimg.com/vi/n5B5q1Hwt_U/mqdefault.jpg)
THESE VIDEOS LITERALLY SAVE MY BIO GRADE LIKE I DONT UNDERSTAND THE WHOLE WEEK IN MY CLASS BUT BAM 8 MINUTES VIDEO AND I KNOW AND UNDERSTAND EVERYTHING THANK YOU
If i ever win a nobel prize i'm gonna give you a shout out, like before my mum even
LOL !!! can u give me a shout out too as I m supporting u in the comment session
@@rittenbrake1613 yeet
If you plan on getting a Nobel your probably never gonna get one
@@SixOhFivewhy not? there’s so many winners who during their careers expressed getting the Nobel prize was part of the achievements to conquer but even if the desire to get the noble was the ONLY reason why someone starts a career in motion it doesn’t take absolutely nothing away from the work done that took you there because is your work that speaks for the worthiness to the prize, not the timeline a person sets a goal for themselves.
Btw @303404jb can I also get a shoutout when you get the noble prize because I’m defending you from unsupportive commenters ?
Oh but after your mum, because mums are always first okay….
Did you win
I didn't have an interpreter on the day I did this lab in my class, and I was so lost! I was nervous to find out that this process was going to be on exam! Yikes! However, I finally understand thank you! The closed caption helped me significantly, again thank you SO MUCH! :)
You seriously cover all of the material that I have googled for my nursing prerequisite classes. Thanks! These are such a big help.
I swear my teacher comes to class half stoned every day, he has no clue how to teach.
Chamblee?
steal all his weed
lmfaooo
I'm really grateful to have found this...It's going to be my first year teaching AP bio, so your videos are very helpful, and answer many of my questions that I have from reading the manuals...
Well, give us an update 7 years later! How is it going?
Thank you!!!!!!!! I wish you were my micro professor. You made my night learning pglo a lot easier
Greetings! Your videos are awesome even after so many years. It would be amazing if you did an updated series!!
You are amazing. One of the worst things about online school is labs, and you got the best solution for it. YOU ARE THE BEST!!!
This video was awesome. I spent a week on this with my IB Bio 2 class and the easy diagrams really helped explain what I was doing in the labs. Thanks, I wish you were my professor!
omg YOURE SUCH A GOOD TEACHER.
this man has saved my life countless times
Thank you so much you seem to make everything simple to me! I always check your page to see if you have a video on all my science problems!
I have these two lab reports due tomorrow and this video just saved my life THANK YOU
Hope you guys pursue biology as a major/minor in college, because, in high school, you study the experiments and what happens, as well as what we now understand. But in college, you get to actually perform these experiments and reinforce what you studied in high school and relearned in college!
I love this guy! I have a Lab test today on 6 labs and we didn't have to hand in this lab so I have no idea what it was about :) Now I do :D
Mr. Paul Anderson I am sooooooo proud of my classes as 8,yes 8, of 14 groups got their bacteria to glow!!
Congrats! Very cool.
Did they feed the bacteria to eat alum limes and potatoes?
@@xiiixiiih.16 huh?
To@@heatherbuckley949
Did the bacteria glow from digesting different foods and preservatives or giant flagitious gases ✌️
just filled like 5 pages of my lab notebook. thanks brotha!
Thank you Mr. Andersen!
I HAVE A LAB REPORT DUE TONIGHT THANK YOU SO FRIKIN MUCH
I take an online AP bio class and I have hardly learned anything this year cuz we don’t have a teacher, just a procter, so this was very very helpful
I am falling in love with Biology
thank you for posting this. I get nervous about labs so I always try to find videos that show me how to do stuff because i get scared of all the stuff i have to do !!
I'm taking college genetics. We were focusing on this topic. This has a very good explanation for what is going on. Thank you!
We did this in class with 5 groups. Everyone got it to transform!
Thank you. Greetings from Bimac Research Group at Universidad del Cauca
Can you do a video on plasmids and transposons. You make it so easy to understand what I am reading. Thank you in advance
k, now let me go fail my bio test :/
you are the freaking best!
I love you so much. I actually understand this now
This is so helpful! Thank you!
Your videos are always helpful! Thanks a lot! :)
Wow I actually understand something for once
Amairani Montiel Same XDDD
u dont lol
i
Awesome! Thanks for posting it... I started to do videos too, but never know if I can measure up to the better stuff out there lol!
Oh Lord! thanku so much!!!
very high quality lesson.
Indeed extremely helpful. Hi hanks a million
What do you use to write? Do you just use a mouse? Or is it one of those draw pads?
thanks i needed this
Kindly, sir. Can you tell me which of these plates are used as control?
What would happen if amp was never added to the +DNA plates?
u r a great man
1:20 . For some reason ,for plate 4 , almost 90% of the lawn glow when shined with UV light .. which contract because it is supposed to glow in clusters . So what went wrong ? Anyone know ??
Hello sir.....ph.d in molecular biology involves animal dissections(like where to extract DNA etc from)too as lam from zoology background....please reply
Please reply
LOL finals tomorrow, almost done watching all 1st semester labs :D
any tips for a successful transformation? our lab groups are competitive lol
Wait how do you know it’s lab 6
Can u pls tell me why GFP will not grow if araC (arabinose) is not there..??
GFP is just a protein, but plasmids are DNA. DNA has to be transcribed into mRNA and then translated into a functional protein. The gene for GFP is useless if there is not a starting point for transcription called a "promoter"--that is the araC that you see in the video. AraC is an inducible promoter, meaning that it is usually off but the presence of some chemical (arabinose) can turn it on. Thus, arabinose will turn on the promoter, which initiates transcription of the GFP gene.
This is a Certified Chewachong Classic
are plasmids always beneficial to bacteria?
Beneficial is relative to the context of the selective pressures of the environment. A common transformation protocol involves the solution mentioned in the video alongside a heat shock, which will shock the bacteria and "trick" them into uptaking dna. What is not mentioned is that bacteria can get rid of useless plasmids during replication, which is why it is important that these plasmids for lab work have a selectable marker (such as one that encodes for resistance to an antibiotic).
because they picked up the ampicillin resistant dna (Pglo)
I thank you my deer!
?
?
yep this is somthin really informative
Why does science make my brain melt! D:
You look just like my principal whose name is also Mr. Anderson!
mine didnt work :( but great explination
I viewed your channel mate, absolutely love the content. Subscribed straight away, We should connect!
Thank You!!!! XD
I'm having trouble learning and absorbing information
Hi Mrs.Taylor
Don Le lol
Don Le lol
haha, same! i just wanna sleep, but i need to finish them tonight
HIII MS. TAYLOR
no
neat.
who watches him at 2x speed lol
And a fluorescent graffiti of disbelief appears on the castle of nature. I hope it won’t be interpreted as a demolition notice:)
CaCl2 is a “divalent cation.” Can you explain why a “monovalent cation” like NaCl would not be as effective?
The reason why divalent cations (basically always Ca++) work is because they can simultaneously be attracted the negatively charged bacterial membranes and the negatively charge DNa. This allows Ca++ to basically bring the DNA closer to the cell. Monovalent cations would only be able to be attracted to one or the other.
MS.CHIANG IS LIFE.
6:00 poor bacteria 😭
greatt
god bless, come teach my bio prof how to do biology. thx.
last minute cram section!
Damn i hate doing these videos
SUS
i wish i didnt take ap bio
same
Rath Lake
dude i still can't science😔
"interpelate"
🍎
6:54 lol
11 dislikes I can't
migrate my bawls-
You need to learn how fun it is for your betters to beat up people like you on-line. You go ahead and tell me why a university with 50K student body wants to do a chemical poration when an electro-poration is so much more efficient. u need to tell me!
Oregon Rexy I have zero idea
Bryan Park
That's ok - big thoughts aren't for everyone.
bıktım bu adamı her pazar gecesi izlemekten YETEEEEEEEEEEEEEEEEEERRRRRRRRRRRRRRRRRRRRR