Microbiology 24 = Pour Plate Method | Pure Culture Techniques | Pure Culture Preparation

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Pour plate method is usually the method of choice for counting the number of colony-forming bacteria present in a liquid specimen. In this method, a fixed amount of inoculum (generally 1 ml) from a broth/sample is placed in the centre of sterile Petri dish using a sterile pipette. Molten cooled agar (approx. 15mL) is then poured into the Petri dish containing the inoculum and mixed well. After the solidification of the agar, the plate is inverted and incubated at 37°C for 24-48 hours.
Microorganisms will grow both on the surface and within the medium. Colonies that grow within the medium generally are small in size and maybe confluent; the few that grow on the agar surface are of the same size and appearance as those on a streak plate. Each (both large and small) colony is carefully counted (using magnifying colony counter if needed). Each colony represents a “colony-forming unit” (CFU).
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[@syedsaif8161]

Nice explanation Sir 👍

[@nakeebahmad3827]

thank you sir

[@shivamgajjalwar1745]

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[@MukeshKumar-uk5pc]

Thanks sir ji

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Welcome

[@garimasuniljangir5185]

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[@wanderlustomar]

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[@wanderlustomar]

I have watched your all videos at least 2 times or more . And also your microbiology lectures are very helpful. You put all your efforts to make the videos without any demand of money. May your novel works flourish in the future. Wish you good health and lots of love from Dibrugarh University

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Thank you so much for your feedback

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[@mohammadrizwan8842]

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[@superbscientist743]

Which media is mixed with agar for pour plate method? Like Luria-Bertani agar, Muller-Hilton agar or any other?

[@hridaysikdar5336]

Thank you sir