Chromosome walking

Chromosome walking is a technique, which is widely used for the discovery for new genes and sequencing of chromosome. For this the chromosome is isolated using pulsed field gel electrophoresis and digested partially to result in overlapping fragments. The fragments are ligated in plasmid and transformed in cells. The transformed cells are allowed to form colonies. In chromosome walking a colony is picked up and the insert is used as a probe for screening colonies having overlapping fragments. When the process is repeated multiple times, a complete sequence of chromosome can be obtained.

[@DrawPassion7654]

Super clear and easy to understand, thank you !

[@ely5858]

thank you thank you thank you.....i have been trying to study WGS and i just couldn,t understand clone contig assembly using probes. this short video made it clear now. tysm

[@yunzhuyu6766]

Great explanaion, clear and explicit! I have a question, will fragment 3 primer also binds to fragment 4?

[@dilipsam4271]

Wow.. Very nice explanation. It shows your command on subject. Can you do video on chromosomes jumping

[@Perpetualteachings]

From 1:36 to 1:42 , 6 seconds of silence bro, it makes the video feel like an hour long 😅

[@soura]

Why do we need the radiolabelling and hybridization? Why can we not simply use the overlapping sequences and make the full length sequence from the subsequences?

[@dhrubajyotidas6258]

That is because of the read length of tge sequencing methods...

[@jeeyaamonkar1199]

Thank you so much

[@deepprabha]

Explaination 👍🏼👍🏼

[@noahperrotte9025]

I love you Indian man