Hemocytometer - Counting of cells - Amrita University

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The hemocytometer was invented by Louis-Charles Malassez. The device is basically a special microscope slide consists of two chambers, each of which is divided into nine 1.0mm x 1.0mm large squares separated from one another by triple lines. The area of each is 1mm2. A cover glass is supported over the chambers at a height of 0.1mm. Thus the entire counting grid lies under a volume of 0.9 mm3 on one side. Cell suspensions are introduced under the cover glass. The hemocytometer is placed on the microscope stage and the cell suspension is counted.

[@TaoOfChi]

Good effort.But you must press the cover slip on the shoulders of chamber gently and firmly giving circular motion to the extent that Newton's rings are visible. This step ensures that cover slip would not fall off and fill volume under the cover slip in the counting area is exactly 1 Cubic mm. thus enhancing accuracy of your count

[@MissEdisonChen]

Thanks, this is brilliant. Very very clear. I've been looking for videos that explained everything, and this video did the job.

[@mmst3620]

Clear, brief and nice presentation.
Thanks

[@pinguswalk]

cleared up some confusion for me. Thanks.

[@oguntakingsley6335]

i counted 20 cells total from the 16 squares.... how do i calculate the number of viable cells?

[@ShivnarayanDhuppar]

What is the song in the background?

[@mandible92]

thank god i watched this video, did this in class today and last tuesday, could not get it right because our teacher went off track and did our heads in

[@dr.a.badruddoza5071]

Thank you very much for the helpful video.

[@atyqabenshahab]

Thankyou :D
this really helped me a lot :D

[@dr.rajkumarbidlan9403]

Good explanation. One important correction that needs to be considered in the presentation: The calculation part carries the formulae wrongly depicted; percentage calculation with your formula would lead to reduction in value by 10000 times.

[@sureshraghav964]

Nice presentation

[@silentbutterflii]

very helpful, thank you ^_^

[@muhammadisa1047]

nice one

[@subramaniansambandam6664]

Spirit is 5%methanol in ethanol

[@nagoormeeravali7417]

thank you very much for this video.can we use haemocytometer to count micro algae

[@Arisudev]

maybe yes but need special preparations to make these cells visible

[@pound4poundpete]

Cool Video. Thanks :o)

[@TheUdayachandrika]

nice

[@colinedb]

Your viability fraction should be multiplied by 100% in the numerator instead of the denominator, but a great video otherwise - thanks!

[@rajashekhar312]

At 4:41, 100 must be in numerator (I think)

[@lyvian0321]

what is the "spirit" used in the procedure?

[@cfhuan]

probably 70% ethanol

[@nagoormeeravali7417]

anonymous can we use haemocytometer to count micro algae i.e. diatoms

[@The_Innov8or]

Good video but are you sure we need to open the box to take out the hemocytometer? :p

[@10001mkr]

Thanks.