Why would anyone want to make fish glow?
HTML-код
- Опубликовано: 29 сен 2024
- The glow of glowing fish comes from the jellyfish Aequorea victoria. The GFP gene from Aequorea victoria was transferred to a zebrafish, where it is expressed in the muscle cells of the zebrafish. Interestingly creating glowing fish for pets wasn't the original purpose of the experiment. Instead these glowing fish were designed to sense toxins in the environment.
If you enjoyed this transgenic content SUBSCRIBE! ▶ | youtube.everyce... | Follow Every Cell a Universe on Twitter ▶ / everycellauni | Playlist of all Every Cell a Universe videos here ▶ youtube.everyce...
Resources :
General information on the zebrafish
rebrand.ly/uc5g6h
rebrand.ly/1tgrnu
History of George Streisinger and zebrafish
rebrand.ly/now7u2
A memoir of George Streisinger
rebrand.ly/75e9ap
George Streisinger's first major zebrafish publication launching the field of zebrafish research. The first step was the creation of homozygous zebrafish, which would allow the creation of zebrafish mutants for research.
rebrand.ly/xan03g
A review article on transgene technology for the production of the human insulin protein. Herbert Boyer, together with the entrepreneur Robert Swanson founded Genentech that became a major in biotechnology and started producing human insulin in bacteria. The review takes a look at the history of insulin extraction and how commercial recombinant insulin production started.
rebrand.ly/upkiqe
(Open source) A fascinating paper by Stanley Cohen taking a retrospective look at the cloning and the impact it has had on biology. The paper outlines well the steps it took to clone DNA and refers to the significant papers. Here Cohen states that researchers suspected that there were barriers between species limiting the transfer of genes.
rebrand.ly/uq345f
The original paper (1962) by Osamu Shimomura and colleagues where they first extracted the GFP protein. They were studying a different fluorescent protein at the time, aequorin, and in the paper, GFP is only briefly mentioned as a side note and didn't have a name yet. Later it was found that in the jellyfish, Aequorea victoria, aequorin releases blue light that excites the GFP protein to glow green.
rebrand.ly/cx5s4g
Another vital paper (1979) by Osamu Shimomura and colleagues describing the structure of GFP
rebrand.ly/lbls5z
The famous paper describing the first transfer of the GFP gene by Douglas Prasher and colleagues
rebrand.ly/ob8g5u
A review of transgenic marine animals
rebrand.ly/x759zu
A review on the use of GFP in zebrafish by Zhiyuan Gong and Haiyan Wan.
rebrand.ly/6qq1gq
The paper on the first glowing fish by Zhiyuan Gong and colleagues
rebrand.ly/0g3882
A paper describing the making of the glowing zebrafish by expressing GFP, RFP, and YFP under the mylpfa promoter.
rebrand.ly/tgfvz9
(Open source) General review of fluorescent proteins as biomarkers
rebrand.ly/zzi3e7
A paper assessing the environmental impact of Glofish
rebrand.ly/mm6ky6
Zebrafish are more vulnerable to predators
rebrand.ly/kumuhu
A review article looking at the different fluorescent zebrafish developed for sensing environmental toxins. According to the article most of the methods created have not been able to detect toxicants at environmentally relevant concentrations
rebrand.ly/62421
A review article on zebrafish and toxicology. The review looks at how zebrafish can be used to study the effects of toxins and also the research their use for toxin detection.
rebrand.ly/lpj500
Zhiyuan Gong's fish was also meant to become toxin sensing fish. This is communicated in the book Frankenstein's Cat by Emily Anthes, which takes a look at transgenic animals in general. According to the author, she gained this knowledge from Alan Blake, one of the founders of Yorktown. In the patent filed by Zhiyuan Gong, the primary object of the fluorescent fish was stated to either be continually glowing or to glow selectively in the presence of chemicals.
rebrand.ly/j0tyg4
rebrand.ly/1coima
The book Frankenstein's Cat is also a source for the information on Yorktown technologies and the time when Glofish entered the market
The selling of GM fish is limited in the EU
rebrand.ly/79rhgl
Credits:
Glofish images are from www.glofish.com
Images under (CC BY 2.0)
rebrand.ly/8386d
rebrand.ly/7d4e1
rebrand.ly/8e802
rebrand.ly/xqzway
rebrand.ly/rm4g7y
Other images
rebrand.ly/qzahkv
rebrand.ly/8a08c
rebrand.ly/mvlmmg
rebrand.ly/lhnpwn
rebrand.ly/1d974
rebrand.ly/a7915
rebrand.ly/gi8qgq
rebrand.ly/ad83b
Zebrafish image under creative commons
rebrand.ly/a4a80
Zebrafish development video (CC BY 4.0)
rebrand.ly/69fc1
I never imagined how intresting glowfish could be!! Your videos are insanely well-made!
What about glowing plants?
@@SylkaChan i don t know
Why? Because it's fuking awesome!!!!
RGB humans when?
It might take a while. Though maybe some biohacker is going to turn themselves technicolor.
Spread the word and help the channel grow! Reddit users, here's a few subreddits the videos might be suited to r/channelwatch, r/curiousvideos, r/educativevideos, r/qualityyoutube and r/videos.
The support you guys give is priceless. Thank you!
Thought this was a kurzgezagt vid. Close enough and good work
Nice, thank you! That's a great compliment!
Fascinating subject, and superb animation. A whole staff couldn't have made any better production than this.
Thank you, glad the hours spent making these pays off!
this was very interesting! but in terms of environmental monitoring, what exactly would be the advantages of using zebrafish instead of just checking with chemical indicators?
The biosensing fish could swim around constantly, and thus, changes would be noticed in real-time. They could probably monitor more than one pollutant, with different colored fluorescent proteins activated with different toxicants. I would think that the fish would also be used in conjunction with chemical tests.
@@EveryCellAUniverse Also, a fish as a biosensor is more likely to be a cleaner/greener approach to environmental monitoring versus having to deal with chemical indicators.
I have bred these guys and now I have more than 90 of them
YO THIS CHANNEL IS CRITICALLY UNDERRATED
THANK YOU FOR MAKING THIS VIDEO
I would a imagine a single glowing fish would be at a disadvantage, but imagine if it was a school of fish with synchronized movements that would just be a mind fuck for predators. Due to how light behaves in water it’ll just be like a giant glowing mass that could possibly blind or confuse other fish.
I worked at both VA's in Palo Alto (Stanford) as well as SF (UCSF). This was intended for human application all along. Today, we call it MVP, Million Veteran Program. That catalogs DNA and maps each specimen while monitoring the health of the patient. Ultimately this is for Genomic Therapy. Not only curing disease at a genomic level, but also having he power to regrow limbs and organs.
thanks i just bought them yesterday from fish market,and wonder how can they be...wow its lots of work to get that. your vdo is very helpful in giving me a clue what i have in my tank.
i thought this came from National University Of Singapore????
Great job!
Thank you!
Duuuuuh cuz it’s cool. Same reason any scientists EVER did BOTHER to even give a suck u kno?)
ah, it's like the quality of your videos are getting better and better!
it's quite interesting how these glofish aren't available in europe, but when i was visiting the states i stumbled across them in a walmart.
funny things, these GMO fish. kinda makes me wonder, is it possible to make a human glow like that? (asking for a friend)
anyway, keep up the good work!
Thank you! Haven't seen them live myself either, would be interesting though! We can make many things glow, just choose the appropriate promoter and we can make pretty much anything glow. Humans included.
And because of that's, now i will have green 🟢 glowing in the dark crayfish
You are the best
Excellent explanation thank you dear .you are the best.
Why would anyone not want to make a fish glow…?
It is fascinating to imagine the creation of entirely new subspecies, their traits determined by human interest rather than environmental pressure. The next step is to make these forced traits heritable.
Making them heritable is automatic. If I remember correctly, the glofish sold for pets can't really reproduce. But this is because they've been pressure treated etc. so people have to buy new ones instead of making the fish reproduce. If this weren't done, the traits would be inherited just like in any other species.
You are also a cute guy I ever seen
maybe because human like that.
oh no I'm late to a video ;-;
Not too late :)
Good video
nice video, sir ! love it ,
What brought me to your video was my idea of how to make my fish glowing. Is it possible if we feed GFP to any fish ? can we produce GFP in a form of fish pallet ? just curious thanks
Amazing information
wowww greattt job man
I love your voice man and really good explanation 😍keep growing god bless you 🤗🥰
Nicely made video. Very informative and a visual feast at the same time. Thank you for sharing the facts about the glow fish in an interesting way
“Aberrant fish”
(Ark reference)
Also.....”glo-triops?”
Thanks a lot
so cool holy
wow
wow!
Indeed!
Kip it up bro very very informative and useful thanks and love
Thanks so much dude. Super interesting and somewhat easy to understand
Great work man.. you cleared my doubt very well.
why do this type of videos does not get more views
this was so well made and well explained. thanks a lot!
I was late 😪😞
No worries! Glad you made it 😊
your channel deserves more subs
Very detailed vid thx
Good video 💪🏾
Very well made!
Awesome
🙂
🙏👍👍👍👍👍👍tqs