In Situ Hybridization

[@manarsmaan2624]

This is how the person explain when he loves what he does .
thank you so much for the great explanation
All Respect

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@renegerritsen6178]

This is the third video I watch on this topic and you are the first to actually make it understandable, thank you!

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@mireillet4573]

This is what you call a good and simple tutorial. Thank you so much !

[@shomusbiologyofficial]

You're welcome

[@wrapupstudio9149]

you are tooo good.... A teacher like you is not even in reputed colleges... thank u so much.. keep going

[@malikamehmood3281]

Tomorrow is my exam, and you know what you are a great teacher and understand each and every thing you've explained, thank you so much ❤️

[@mmmmmmmmm4383]

Thank you for appreciating his efforts

[@umeaiman4168]

Sir, u r one of my great teachers...my every problem has solution from ur lectures...

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@stephanie_4203]

This is awesome. Why I can't find teachers like him¡¡ I understood all¡¡¡ Thank you so much. I will approve my exams now¡¡¡

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@maryamjokar7066]

4:10 now that was a perfect circle 😳

[@shomusbiologyofficial]

Thank you

[@muskangarana8074]

You are really master of microbiology
Thanks a lot for the videos 🙏

[@shomusbiologyofficial]

You're welcome

[@xeverydaynormalgirlx]

My favourite part of this video was the part where an ad popped up offering to do my assignments for me. I bet that will help my learning.

[@yasirahmad2185]

Sir, i m pakistani and loves u sir for ur simplicity and teaching method. InshAllah in future i will come to india specially to meet u sir.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

[@yaseenkhan-im9ti]

Aap jaise logon ki wajah se genetics interesting hai

[@shomusbiologyofficial]

Thank you

[@coconuttree1972]

Sir, You're really something.... ❤🎉🎉🎉

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@anj19911]

AMAZING!! BETTER THAN MY SCHOOL TEACHERS!!! THANK YOU!!!!!!!!!

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures. Please subscribe and share

[@SunandGold]

Thank you!! Really helpful, I was so confused by all the websites I used because they over-complicated the terminology and the diagrams! I spent so much time on those websites getting confused, when I could have just watched your video

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@jijizali]

NOBODY EXPLAINS ABT FISH BETTER THAN THIS VIDEO OF YOURS SIR THANK YOU SO MUCH

[@shomusbiologyofficial]

You're welcome. Glad you like it

[@rakshithav2271]

Thank you so much... trying to prepare for exams. And your videos make them so much easier.

[@nyctophile1841]

Amazing way of explaining sir.. Thank you sir for this 🥰

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@hesnanoussa6442]

i do study biology ( biochimestry )but in frrench luckely ur explanation was quite good i have understood quite well

[@ushakarreddy1981]

excellent sir ..my doubts were cleared by your wonderful lecture..

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@CelestialCookies]

God bless you. You make biology as simple as it should be. Thank you very much

[@chintanmehta4695]

Hats off sir! I am speechless that the way you narrate is amazing one.

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@seepa3890]

It is easy because you explained it in simple way☺️

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@khushshah524]

I am presenting this technique tomorrow in front of my PI. This guy saved my life. I have a question: what does hybridization mean in HCR? Which step in this process is
called hybridization?

[@fikayoadegboyega3580]

Nice one. I so much like your teaching skills

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@yimuyang4101]

Thank you Shomu!! Your lecture make it easy to learn, Big help!!!

[@biologywithbasira]

great job! your explanation is amazing

[@shomusbiologyofficial]

Thank you

[@diegonzalez25]

Muchas Gracias Shomu!!! Saludos desde Paraguay

[@tatyanakiryutina7778]

Thanks! Most helpful information I found so far!

[@shomusbiologyofficial]

You're welcome

[@DHIRAJKUMAR-xv4sr]

Helpfull for me all six lecture related to gene mapping......thanks sir .😊

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@chhoamondal3526]

I have understood easily the complex thing from your lecture

[@shomusbiologyofficial]

You're welcome

[@alonir101]

Thanks man, you are great and i like your passion about this :)

[@briabriapia]

dam that circle was perfect

[@shikidixit5144]

Thank you, Shomu!

[@shwetagupta9004]

Thank u so much sir . Aap n hote to pata nahi hum students ka kya hota.

[@shomusbiologyofficial]

You're welcome

[@zunairaakram1672]

Thank you shomu...helped me alot,,, cheers from pakistan... :)

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@RahulKumar-mm5vm]

I have a question regarding dna sequence using FISH. "Is it possible to identify a particular gene sequence (for example - Amel Y) using FISH if there is lot of depurination (loss of adenine , guanine) in interested gene sequence ?"

[@emilymarques6739]

Thank you Shomu!

[@shomusbiologyofficial]

You're welcome

[@mayukhroy5393]

Wonderful explanation Sir.

[@shomusbiologyofficial]

You're welcome

[@AnimeUni-versed]

You are a blessing !

[@shomusbiologyofficial]

Thank you

[@arias.p4355]

Thanks a lot! Your videos are amazing!

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@RajKumari-ld7ld]

Thanks brother.... U r a brilliant teacher

[@prathameshdeshmukh5844]

REALLY HELPFUL....... AWESOME LECTURE.... TY..

[@shomusbiologyofficial]

Glad it helped

[@latimerpaul]

Great video, Shomu. Thank you!

[@shomusbiologyofficial]

You're welcome

[@sramanagiri3803]

Ur explainations are so worthy to listen

[@dasachomova201]

Thank you, all your videos are very helpful :)

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@NehaSharma-hd2so]

U r gem to me and others too.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@mansiwadekar1719]

Thank you
Very well taught

[@shomusbiologyofficial]

You're welcome

[@giovannaagnelli6752]

Thank you so much! It was so very hard to find a good video about in situ Hybridization. 🍀🔬📚💘

[@mahaswetaguha-u4m]

You r very helpful bro

[@iancuharghel8532]

I hope you are doing fine! You are great!

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from

[@jim5400]

Great job Shomu!

[@mahyahosseinib.4366]

Many thanks, Shomu!!!

[@kouapatrick3838]

Thank you Shomu! I would like to know how the 2 strands of the DNA is separated in this process? by heating the cell ?

[@ruthzomuansangi9698]

KOUA Patrick i think nick translation is used.

[@prachikamble1004]

Good explanation skills! I like ur all the videos!

[@aishasoliman8647]

really helped!! thanks a lot
I have a question:
If I have wild type and mutant models and I wanna examine the expression of specific markers Should I prepare probes for each markers for both mutant and wild type ?

[@mohammadnamarneh2345]

You are amaizing 🌷🌷🌷🌷🌷🌷

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@alemranhosen1531]

Love you from BD🇧🇩

[@shomusbiologyofficial]

Thank you

[@danielhain40]

PLEASE MAKE MORE!!!

[@shomusbiologyofficial]

Sure

[@ShahFahad-eo6fm]

It was very helpful bro ,thank you for making the video

[@chelseagunaratna5249]

Hi Shomu! You are amazing!

[@HCLpradeep2010]

Superb Boss!

[@shomusbiologyofficial]

You're welcome

[@nehanasir3492]

How is the complementary strand to the target sequence removed before hybridization?
Because it may happen that during hybridization or cooling, the target strand again bind to the complementary sequence instead of probe

[@TIB5]

fantastic video, thank you so much!!!!!keep up the good work. Im subscribing already!

[@shomusbiologyofficial]

+-TIB5- thank you. Glad you liked my lectures

[@rahishaaz4392]

Sir is FISH technique used for serotonin binding receptorss???
Plzz answer

[@normadicn5700]

Google?

[@supriyaubasupriyauba3217]

Thank you so much sir

[@shomusbiologyofficial]

You're welcome

[@priyankatiwari3434]

thanks .....great video as always..

[@totallyomi]

Sorry if this is a stupid question but how do we know the DNA sequence we are targetting in order to design the complimentary probe?

[@shomusbiologyofficial]

Dna sequence must be known prior to the experiment

[@moonsevinci88]

Thank you. Plz how we can separate the strands ? in vivant

[@kimlynnybaby]

thank you, you were extremely helpful

[@kirtisharma8609]

Tysm🤓😊great video...👍

[@shomusbiologyofficial]

Glad to hear that you are getting benefit from the videos

[@ci7672]

Thaaank u a lot teacher

[@shomusbiologyofficial]

You're welcome

[@Homosap12]

Thank you ❣️

[@shomusbiologyofficial]

You're welcome

[@reviewit9100]

Very helpful video for mee😍😍

[@sapirshaba7546]

Hello, can you explain about detecting abnormal chromosomes such as 18,21, by FISH?

[@soumyasudhi3955]

Useful video....Thank u..

[@davidcharles6518]

Thanks very helpful👍

[@arshiarshi3756]

You are right 👍 it is a easiest method.

[@danielnguyen123]

THANKS SHOMU ;) ❤️❤️

[@shomusbiologyofficial]

You're welcome

[@harshithan.jadhav5294]

Thank you so so much ✨

[@shomusbiologyofficial]

You're welcome

[@dr.bidouranaznin1339]

Thanks! nice & simple way to learn. How much work area is required for FISH setup?

[@markj.7687]

FISH set up is basic and does not require much bench top space. a simple table top would work just fine.

[@AS-mm4pn]

What exactly did you mean by freezing the mRNAs?And how can you separate the 2 strands of a dsDNA?

[@oinambobochandsingh89]

Wonderful

[@shomusbiologyofficial]

You're welcome

[@neelamdas5565]

soooo nyc bro.... help me a lotttttttt...... yaaaa a lottttttttttttt

[@mandisangema2568]

how do you know the complementary sequence of the gene i.e. the probe or bait?

[@bikramroy3783]

Sir can you tell me which book it is present

[@rashmisingh1857]

As i am pursuing PG.....wanna more elaboration of topics.......it wz lil bit helpful

[@nithyaa5841]

Sir what about in situ hybridization using non radiolabeled probes??

[@KIRUBAKAR]

Bro what's the difference between colony hybridization and insitu hybridization

[@mauliksadhu8963]

Is it not same like southern blotting...?
What's the difference between these two..?

[@sanaznoormohamadi9250]

perfect special regards

[@shomusbiologyofficial]

You're welcome

[@varshabhatt7414]

Sir uhh also provide notes????

[@shomusbiologyofficial]

No. Study materials which is paud service

[@saimairshad5074]

Sir, is this same as southern and northern blotting techniques?

[@jyotiyadav9930]

very helpful this vedio for me ✌✌✌

[@SMS.WAHEED]

Awsome sir but how know the gene sequence?

[@rajshreepal6636]

thank u sir.....

[@shomusbiologyofficial]

You're welcome

[@mohsinbukhri9864]

well done bro...

[@shivanisehra1487]

Mere exams h next month please ap kuch topics h unpe video bna skte ho plzzzzzzzzzzzzz mujhe smjh nhi aa rha kuch

[@abhasingh6959]

how do u arrest cell in metaphase...when done on slide? reagent and concentration please...Nd please elaborate on signals .

[@florytalez]

We arrest them using freeze fracturing technique

[@AsenathWaiteDerby]

thank you so so much, I got it :))))

[@shomusbiologyofficial]

You're welcome

[@pagalchori577]

thank u shomu sir ......