In Situ Hybridization

This lecture explains about the in situ hybridization process and the role of in situ hybridization techniques in identifying specific DNA sequence.
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[@RahulKumar-mm5vm]

I have a question regarding dna sequence using FISH. "Is it possible to identify a particular gene sequence (for example - Amel Y) using FISH if there is lot of depurination (loss of adenine , guanine) in interested gene sequence ?"

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I am presenting this technique tomorrow in front of my PI. This guy saved my life. I have a question: what does hybridization mean in HCR? Which step in this process is
called hybridization?

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Sir is FISH technique used for serotonin binding receptorss???
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Hello, can you explain about detecting abnormal chromosomes such as 18,21, by FISH?

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[@giovannaagnelli6752]

Thank you so much! It was so very hard to find a good video about in situ Hybridization. 🍀🔬📚💘

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[@kouapatrick3838]

Thank you Shomu! I would like to know how the 2 strands of the DNA is separated in this process? by heating the cell ?

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KOUA Patrick i think nick translation is used.

[@aishasoliman8647]

really helped!! thanks a lot
I have a question:
If I have wild type and mutant models and I wanna examine the expression of specific markers Should I prepare probes for each markers for both mutant and wild type ?

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[@moonsevinci88]

Thank you. Plz how we can separate the strands ? in vivant

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Great job Shomu!

[@nehanasir3492]

How is the complementary strand to the target sequence removed before hybridization?
Because it may happen that during hybridization or cooling, the target strand again bind to the complementary sequence instead of probe

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Useful video....Thank u..

[@SMS.WAHEED]

Awsome sir but how know the gene sequence?

[@ShahFahad-eo6fm]

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[@mauliksadhu8963]

Is it not same like southern blotting...?
What's the difference between these two..?

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Thanks

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[@AS-mm4pn]

What exactly did you mean by freezing the mRNAs?And how can you separate the 2 strands of a dsDNA?

[@saimairshad5074]

Sir, is this same as southern and northern blotting techniques?

[@KIRUBAKAR]

Bro what's the difference between colony hybridization and insitu hybridization

[@totallyomi]

Sorry if this is a stupid question but how do we know the DNA sequence we are targetting in order to design the complimentary probe?

[@shomusbiologyofficial]

Dna sequence must be known prior to the experiment

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[@mandisangema2568]

how do you know the complementary sequence of the gene i.e. the probe or bait?

[@dr.bidouranaznin1339]

Thanks! nice & simple way to learn. How much work area is required for FISH setup?

[@markj.7687]

FISH set up is basic and does not require much bench top space. a simple table top would work just fine.

[@nithyaa5841]

Sir what about in situ hybridization using non radiolabeled probes??

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