Warm greetings. The short video is very helpful . But how to identify the specific complementary site in a chromosome . Or how do we know to prepare the complementary probe , on what idea this can be produced? If possible kindly send the answers. Thank you. Dr. Rise de leema .
English is my second language and the way you talk very slow was just amazing for me, because I'm studying about FISH in my university in Brazil and I'm so proud of me to studying in English. By the way I have a test today wish me luck!
I have a question regarding dna sequence using FISH. "Is it possible to identify a particular gene sequence (for example - Amel Y) using FISH if there is lot of depurination (loss of adenine , guanine) in interested gene sequence ?"
2 Quick questions 1) how can the ligase seal the fluorescent nucleotides. They need to be bound first right? So my question is how do these labelled nucleotide bind? DNA polymerase will just randomly come along? 2) if i do the labelling before I amplify, how will the PCR be able to copy the fluorescent dntp? thank you
in my opinion, ligase has some recognition site that recognizes the specific sequences and then it binds and causes ligation. DNA polymerase also has some specific sites that recognize first and then do polymerization. is this correct?
some time it gives false negative results because some time probe are not properly designed and sometime there is change in annealing temperature ? Am I right sir or any other reason of false negative result ?
Thanks. I need to know how to figure out the used probes! For example: we have thousands of syndromes, do i have to make probes for all of them (especially if I have no idea about symptoms)?
Is it correct that a ARRAY CGH is kinda a reversed FISH? U should def do a video about micro array especially the array CGH as it is also used for chromosomal DNA deficts :)
I really do appreciate you Dr Igudia youtube,your commitment towards saving human lives is steadfast ,all the knowledge I got from you and,the help with the herbal meds, I was recently tested negative of Hepatitis B infection,I’m so happy thanks doc
First of all: Good question! My answer is based on this paper: www.sciencedirect.com/science/article/pii/S259000721830008X If you make sure that the concentration of the probe is way higher than the concentration of the target dna (e.g. 10000 probes per dna strand) it should be way more likely that one of these probes bind after denaturation compared with the single sister strand of the dna Hope you could understand my explanation!
That is a good question, but here is the answer: So nick translation is just the process of getting your labelled probe. With this probe you can do whole process of hybridization then (FISHybridization).
It is to detect specific sequences on the patients chromosome (which are altered in disease). With FISH we make this sequence of interest visable using a "fluorescent probe".. this labels the sequence of interest
![Fluorescence insitu hybridization [ FISH ] | Made easy | Physical mapping | Bio science](http://i.ytimg.com/vi/7mspOyKkaDc/mqdefault.jpg)
Which topics should be next? Please make suggestions here:
DNA fingerprinting
Microarray
Bacteriophage typing method
Karyotype versus FISH versus microarray versus targeted exome vs clinical exome
Qq%
1%q@a1@100
This is the best video about FISH that I saw on RUclips!
Extremely awesome and relevant to the preimplantational genetic testing environment. Well done, clearly explained!
Thank u. Nice german accent ;-)
Why the wink
@@thetrollpatrol8799 ;-)
Nice explanation, You made the concept easy and digestible, thanks
This video is very helpful to understand FISH easily
Cell fixation
Adding probe
Denaturation
Hybridisations
Very nice for understanding thank you
It's so helpful and clear video, thank you so much really
So cool! Thank you!
Thanks!
Thanks. Is it possible to tell which test do I have to do when I'm facing a syndrome?
I would ask that a medical doctor!
Gibt es auch ein Video auf deutsch ?
Warm greetings. The short video is very helpful . But how to identify the specific complementary site in a chromosome . Or how do we know to prepare the complementary probe , on what idea this can be produced? If possible kindly send the answers. Thank you. Dr. Rise de leema .
Usually you know the sequence of the complementary site since it is a gene you can look up in genome databases!
Best
Henrik
How you can amplify the flourocent probe by PCR? it wouldn't increase the amount of the probe which is tagged
Does interphasic fish allow us to detect both anuploidie and microdeletions or just anuploidie?
Denaturation
Seek Christ Jesus YHVH.
English is my second language and the way you talk very slow was just amazing for me, because I'm studying about FISH in my university in Brazil and I'm so proud of me to studying in English. By the way I have a test today wish me luck!
Excellent work, thank you very much for your time and effort.
I have a question regarding dna sequence using FISH. "Is it possible to identify a particular gene sequence (for example - Amel Y) using FISH if there is lot of depurination (loss of adenine , guanine) in interested gene sequence ?"
How the deletion site is known . And what confirms that probe is bound like how it gives signal after binding to dna
Thank you totally clear my confusion! and right to the points!
So helpful and clear, thanks!
Where is the DNA that the probe originates come from?
The DNA probes for FISH can be ordered by companies which will synthesize them. Is that what you mean?
This helped me so much thank you for explaining in such a calm voice and tempo
@Mixkopf 38 it helped me lmao
really clear and helpful....solved my problem about why we used small DNA fragments ,thanks a lot
This was soooo helpful,,thank you
2 Quick questions
1) how can the ligase seal the fluorescent nucleotides. They need to be bound first right? So my question is how do these labelled nucleotide bind? DNA polymerase will just randomly come along?
2) if i do the labelling before I amplify, how will the PCR be able to copy the fluorescent dntp?
thank you
in my opinion, ligase has some recognition site that recognizes the specific sequences and then it binds and causes ligation. DNA polymerase also has some specific sites that recognize first and then do polymerization. is this correct?
An exemple of how to design a probe for a specific gene will be nice
Crazy German scientist over here.
Thanks tho, it was very helpful!
Never thought this is how fish are made.
After denaturation, why will the target chromosome will hybridized with the probe instead of rebinding to its original strand?
some time it gives false negative results because some time probe are not properly designed and sometime
there is change in annealing temperature ?
Am I right sir or any other reason of false negative result ?
Thanks a lot :))) You just saved me 3 hours 🌟🌟 Keep up the good work !!
I don't understand... You never explained about the fish. Clickbait
Really Thank u so much sir for this video ☺️ it helped me a lot to do my seminar ....God bless you 🙏
You try to give the video more brightness it will be great if you do
thank you very much for this video!!!!,I understand all
Very helpful! have you done one of CISH?
Helped a lot thank you so much☺️
thank you
Thank youu !!
That was very helpful, thank you
👏👏
Thanks, that was easy to follow and understand.
👍
very good video.
Wow man very nicely done
It was very helpful for me Thank a lot.
anirudh my thank YOU for watching it and the feedback!
Can FISH detect mosaicism in an adult?
Thank you! Really helpful!
Thanks. I need to know how to figure out the used probes! For example: we have thousands of syndromes, do i have to make probes for all of them (especially if I have no idea about symptoms)?
Sir I want videos for chromosome walking
This video was real good!
Wonderful easy to understand video! Thanks Henrik!
Fluorescent in situ hybridization
Thank u sir
Fixation of cell et formaldehyde
Is it correct that a ARRAY CGH is kinda a reversed FISH? U should def do a video about micro array especially the array CGH as it is also used for chromosomal DNA deficts :)
I really do appreciate you Dr Igudia youtube,your commitment towards saving human lives is steadfast ,all the knowledge I got from you and,the help with the herbal meds, I was recently tested negative of Hepatitis B infection,I’m so happy thanks doc
very helpful
Thanks very much ❤
THANKS! NEEDED FOR A PAPER
Useful sir 👏
Very clear n concise... Thanks a lot really
thank you !!
THANK YOU 👍👍👍
Nice accent man!
This is great!
Greatly well explained
This video was so helpful, thank you for your effort
So FISH can help in karotyping too?
Yes
you sound soo german :)
Did anybody learn this in grade 12? because in lebanon we do .
Yes I do. I'm learning this as a new chapter.
@@sylvia_forest ah ok thx
Add probe
do you heat the cells/tissue up to 95 C? will the cells/tissue be destroied by such a high temperature?
sprich doch deutsch henrik
very detail and clear. thanks a lot
Thank you
Thank sir explain mutations.types like this.
how to make sure that the probe is fully hybridized not the complimentary DNA ? thanks :)
First of all: Good question! My answer is based on this paper: www.sciencedirect.com/science/article/pii/S259000721830008X
If you make sure that the concentration of the probe is way higher than the concentration of the target dna (e.g. 10000 probes per dna strand) it should be way more likely that one of these probes bind after denaturation compared with the single sister strand of the dna
Hope you could understand my explanation!
Thankyou!
it's really interesting, please stay connected :-)
Thank you:)
Very helpful thank you so much!
Please hindi me bolo
THANKS FOR YOU
Is Nick translation And FISH hybridization is same
That is a good question, but here is the answer: So nick translation is just the process of getting your labelled probe. With this probe you can do whole process of hybridization then (FISHybridization).
What is the principal and the concept of the FISH technique ?
It is to detect specific sequences on the patients chromosome (which are altered in disease). With FISH we make this sequence of interest visable using a "fluorescent probe".. this labels the sequence of interest
@@henrikslab u help me , thank you SM😣🤍🤍🤍
Kurz und knackig, danke!
THANK YOU!