No, I mean from your point of view you are thinking that its hapening in the yeast so its in vivo. But thats not true. Lets say you have 2 human protein and you can check for direct inteaction in the yeast. So yeast is here just an external system but you cant recreate human cellular environment in yeast. So its in vitro.
Ok thanks.. Could you please make one video on how to design primers for clonning? I mean I always get confused regarding the in frame and out of frame concept. Also, how to tag the protein like flag tag, myc tag etc. If you could make a video, please do.. Thanks 🙏❤
Ok I will make a elaborate in another video , but 8n Short, the input is the total protein lysate and what is eluted from the beads after the immunoprecipiation
RAJANSHI MISHRA it’s not used in that sense , here yeast is just a readout.... using yeast as a system you want to understand whether two proteins can possibly interact in a in vivo set. Up
It is honestly not that bad. This guy is doing the best he can, sharing his knowledge with people for free. Maybe we should thank him instead of criticising him for something he can't help :)
Please watch other vedios .....please share among friends and help me to reach big audience
Bro, isn't yeast two hybrid system is in vivo method ?
No, I mean from your point of view you are thinking that its hapening in the yeast so its in vivo. But thats not true. Lets say you have 2 human protein and you can check for direct inteaction in the yeast. So yeast is here just an external system but you cant recreate human cellular environment in yeast. So its in vitro.
@@biswahereemahananda7894 so its very perspective dependent whether you call it in vivo or vitro
Ok thanks.. Could you please make one video on how to design primers for clonning? I mean I always get confused regarding the in frame and out of frame concept. Also, how to tag the protein like flag tag, myc tag etc. If you could make a video, please do.. Thanks 🙏❤
And yeah your videos are really easy to understand..😍 Came across it recently..
I can see your hard work through this ...
Good going...!!! Thanku
Was really good presented and explained. Well done 👍🏻
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I have a question, which technique can be used to identify protein-protein interactions with HSP90??
OOO Bhai thank you so much
max ruphio please share
It's nice video to understand.thanku so much arpan
Do watch the whole biology technique playlist.... it would be beneficial.... every video is designed to have maximum impact in minimal time
Hi, Thanks for the video ! I didn't understant what input (2:19) mean ?
Ok I will make a elaborate in another video , but 8n Short, the input is the total protein lysate and what is eluted from the beads after the immunoprecipiation
@@animatedbiologywitharpan okay thank you ! Your video is very great and well explained !
THANK YOU SO MUCH
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First method is used in vivo or vitro
Can you say this method is like BioID?
I don't think so
very nice, thank you 🤗
sir invitro means outside of the living organism but yeast to hybridisation system to yeast k inside ho rha h plzz clear my confusion?
RAJANSHI MISHRA it’s not used in that sense , here yeast is just a readout.... using yeast as a system you want to understand whether two proteins can possibly interact in a in vivo set. Up
Moreover cell culture stuff / yeast/ bacteria are conventionaly termed as in vitro... so it’s all the game of linguistics
and sir western blotting to in vitro system h , right?
Yes
oh this terrible accent
It is honestly not that bad. This guy is doing the best he can, sharing his knowledge with people for free. Maybe we should thank him instead of criticising him for something he can't help :)
Is the accent more important or the way of explaining?? The content, the topics and their stratification and analysis??? His accent is far far better.