My research interest since 2003, has been in the study of the DNA Tm, BD, and %GC (which is a biological constant for the genome of a species De Ley, 1970) of DNA. By the way, your Tm formula was first discovered by Julius Marmur and Paul Doty (Marmur & Doty, 1959, 1962) and so has its foundation dates back more than 6o years. Very soon the world will come to know what genius Paul Doty (1921-2011) and Julius Marmur (1926-1996) were. Thank you for sharing, Shomu.
what if I have tow different length in (F) and (R) primers and different (%GC) content Which annealing temperature must be choosed in the PCR protocol,please I need your answer
My research interest since 2003, has been in the study of the DNA Tm, BD, and %GC (which is a biological constant for the genome of a species De Ley, 1970) of DNA. By the way, your Tm formula was first discovered by Julius Marmur and Paul Doty (Marmur & Doty, 1959, 1962) and so has its foundation dates back more than 6o years. Very soon the world will come to know what genius Paul Doty (1921-2011) and Julius Marmur (1926-1996) were. Thank you for sharing, Shomu.
thank you very much
From where 64.9,.41 and 500 comes is there some derivations?
what if I have tow different length in (F) and (R) primers and different (%GC) content
Which annealing temperature must be choosed in the PCR protocol,please I need your answer
500 or 600?