Cell Thawing

( www.abnova.com ) - The thawing procedures for regular (non-DMSO-sensitive) cells are performed. The cell thawing procedure is stressful to frozen cells. Time is the most important factor. Thaw frozen cells rapidly (less than 1 minute) in a 37°C water bath and work quickly can ensure high survival rate of the cells. More videos at Abnova www.abnova.com

[@pharmabaghdad]

Is this applied to cancer cell line too?

[@kosheeka]

Yes, you can use the same cell thawing procedure for cancer cell too!

[@sadiahaquekhan6003]

How I kmow DMSO will be under 1% after adding cells? How to do the calculation?

[@kosheeka]

To ensure DMSO is under 1% after adding cells to suspension media.
We are assuming that the freezing media is made up of 10% DMSO, 90% FBS.
Identify volumes:
• Let V1 be the volume of cell suspension.
• Let V2 be the volume of cryovial freezing media added.
Formula:
• Final DMSO concentration = (0.1 x V2)/(V1 + V2)
Inequality for DMSO < 1%:
• 0.01 > (0.1 x V2)/(V1 + V2)
Simplified condition:
• V2 < (V1)/9
Add less than one-ninth the volume of the cell suspension in freezing media to keep DMSO under 1%. For example, if you have 9 mL of cell suspension, add less than 1 mL of cryovial freezing media.