Cell Counting

[@gasperkosmac7672]

Thank you for the formula in the end

[@Linhmeo93]

I have a question. The step in 2:56, you mention "Add appropriate volume of fresh medium and mix well", what is fresh medium here? What does it compose of?
Thank in advance

[@ashjoe3591]

Your own medium with which you culture the cells before.

[@sananoor1217]

Can i use ordinary light microscope here?

[@kosheeka]

Yes, you can use ordinary light microscopy for counting the cells.

[@marianabm100]

What about the deactivation of trypsin?

[@kosheeka]

Deactivating trypsin is essential to prevent further cell damage after detachment. Adding complete growth medium containing serum effectively inactivates trypsin. The serum components, such as alpha-1-antitrypsin, inhibit trypsin's proteolytic activity.

[@강현아-h6s]

can you tell me about cell line name? i really want to know that..

[@TheBenjaRamirez]

Vero cells (adherents)

[@fory7781]

3:58

[@하늘-m2j2b]

시청완료!

[@Chittradugra.pigeon_]

Supper 0:16