Oh my God I can't believe you guys. This professor is teaching an important subject in microbiology and you're trying to hit on her? Ugh you disgust me. Dr. Holden thank you very much for this informative video! It helped me a lot. keep going.
aside from measuring dilute samples, another good thing about MPN is that it's activity based which measures alive cells only - under the microscope an extra step of staining might be necessary which can be sometimes tricky because those staining agents usually only work within a certain narrow pH range
MPN is a guess on cfu/ml based on statistic. While yes we calculate out MPN is 33 cfu/100ml but without actually counting the colonies we can't simply say there is exactly 33 cfu/100ml in our water sample. Which is why we have 95% Confidence Interval (2SD). To say "while 33 cfu/100ml is statistically our best guess, we can be 95% certain that the actual cfu/100ml is b/w 11 to 93 cfu/100ml.
But as per my std. Book it says different dilution of sample of water is mixed with lactose broth and incubated intest tube for 48hrs at 37 degree Celsius
mam you are so so cute , thatswhy i understand whole the concept related to mpn test just see you on board. I'm a civil engineering student and our environment engineering, this concept are also include , thank you so much
Coliforms ferment the lactose to produce acid AND gas. The production of acid is indicated by color change and the production of gas is detected as gas bubbles collected in an inverted durham tube. So if you are trying to quantify coliforms, you need both. A color change without gas production probably means a different kind of bacterium that can produce an acid, but not a gas, in that environment.
I wish I had such beautiful teacher in my college.❤️❤️❤️ I would never miss your single class. Your explanation is very easy to understand mam. Bdw I am 26😂😂
Because the original sample is so dilute (i.e. has so few bacteria in it), that you can transfer some liquid to the test tubes without actually having any bacteria transferred as well (resulting in a negative result)
This method is suited best for very dilute concentrations of coliforms, meaning not very many present in the sample. There are typically a lot in contaminated soil and so different methods are used instead.
you look for that pattern on the table. 4-3-1 under combination of positives and then 33 under MPN index. you probably don't need this answer a month later, but hey maybe it'll help someone else lol
Are you just adding closed captions to my video in Kurdish and then sharing the video on your RUclips channel? I think that is fine, but please do not upload a separate copy.
You are a fucking douche...she is not on here making self tutorials or biographies...asking for how you think she looks...she is a Professor TEACHING MICRO..I can not believe this is how women are openly treated..sickening
This is the easiest explanation of MPN I have seen. Thank you Biology Professor
Oh my God I can't believe you guys. This professor is teaching an important subject in microbiology and you're trying to hit on her? Ugh you disgust me.
Dr. Holden thank you very much for this informative video! It helped me a lot. keep going.
It is very difficult to avoid beauty
Hi. I searched MPN for my class assignment and am surprised to see himym's Victoria teaching in the video!❤️😅
Subscribed instantaneously!🔥❤️
And I was wo wondering all along who she looks likeeee xD
OUTSTANDING TEACHING!!!
ALL THE CONCEPTS ARE CLEARLY DELIVERED.
aside from measuring dilute samples, another good thing about MPN is that it's activity based which measures alive cells only - under the microscope an extra step of staining might be necessary which can be sometimes tricky because those staining agents usually only work within a certain narrow pH range
Well explained...
But it would be great if you make a next vedio on how to read and interpret the MPN results
She did explain how 1 MPN = 1 CFU
This was very helpful to understand the MPN procedure for measuring total coliform. 10/10
Thank you so much! i like your way of teaching, it's no overly complicated and the visual explanation was good.
Very easy and extraordinary explanation .... Keep uploading this type of videos..... So that we can learn easily.
Thank u mam ...for clearing the concept of MPN test .....
Thank you for understanding
Now I know that 1MPN =1CFU..tnx prof..
This video is excellent. Excellent presentation and very easy to follow.
Thanks!
1)What is the vol of LB for each tubes?
2)how to make ds and ss broth?
Thank you mam for such a easy explanation of such a complex topic.
Thank you so much, I watched and had a better understanding, it was my number one question in my exams, so I did justice to it, 🥰
Thanks!
Wonderful explanation
Thanks!
Why is there a big range (11 to 93) if MNP is 33 in this case, for example.
MPN is a guess on cfu/ml based on statistic. While yes we calculate out MPN is 33 cfu/100ml but without actually counting the colonies we can't simply say there is exactly 33 cfu/100ml in our water sample. Which is why we have 95% Confidence Interval (2SD). To say "while 33 cfu/100ml is statistically our best guess, we can be 95% certain that the actual cfu/100ml is b/w 11 to 93 cfu/100ml.
Thank you miss. Take love from bangladesh
Thank you for the visual graphical explanation, that's very helpful!
Regards from NIT Kurukshetra, India
Thanks prof its very clear
this was very helpful ...thank you
Muchas gracias señorita
Thanks a lot professor
Thanks! This is very helpful and well explained!
this is very fantastic
But as per my std. Book it says different dilution of sample of water is mixed with lactose broth and incubated intest tube for 48hrs at 37 degree Celsius
Tks
thanks this was very helpful!
Thank you so much ❤️❤️
Well explained.
Thanks miss.
helpful biology lecture
Great explanation, thanks.
well done. Thank you.
you're a life saver!
how do you interpret a a series that is not in the reference table? is it invalid?
So Good lecture
mam you are so so cute , thatswhy i understand whole the concept related to mpn test just see you on board. I'm a civil engineering student and our environment engineering, this concept are also include , thank you so much
concise and informative! thank you for sharing :)
Amazing! Thank you so much! 😊😊
Thank u mam... Love u for teaching mpn
Just amazing❤️ loved ur explanation
Thanks for telling me! 🥰
You save me !!
Thx a lot
It helped me a-lot thank you so much
Thank you so much, i needed this.
I’m glad it worked out!
Very informative video thanku mam from india jammu
Thanks Dr
thats really sweet teaching...thank u mam....
thanks
You're welcome!
Good
😊
Thank you so much mam......
If there is a change in colour but no release of gas, is it positive or negative? Please answer with explaination
Coliforms ferment the lactose to produce acid AND gas. The production of acid is indicated by color change and the production of gas is detected as gas bubbles collected in an inverted durham tube. So if you are trying to quantify coliforms, you need both. A color change without gas production probably means a different kind of bacterium that can produce an acid, but not a gas, in that environment.
god job. Thx for helping
now you are healthy in 2020... looking nice. slim and smart in 2018...
Super explanation
Wow 🔥🔥🔥♥️
Hello Prof. Just a clarification, I will inoculate 10ml, 1ml and 0.1 ml from the original sample?
Thank you mam😍
Ma'am I feel it difficult to understand this topic in 7 minutes. pls make a video of atleast 70 minutes 🙃😍
Sorry... maybe watch this one 10 times? ;)
Ma'am plz make vedio on chromatography & spectrometry& also on electrophoresis
The given topics are well explained in this website: www.chemguide.co.uk/analysismenu.html
I wish I had such beautiful teacher in my college.❤️❤️❤️ I would never miss your single class. Your explanation is very easy to understand mam. Bdw I am 26😂😂
Thanks mam
Best 👍👍👍💐
If we take the same sample of 5 test tubes of 10 ml means, then why some are +ve and some are -ve results?
Because the original sample is so dilute (i.e. has so few bacteria in it), that you can transfer some liquid to the test tubes without actually having any bacteria transferred as well (resulting in a negative result)
Thank u, mam for clarifying my doubt.
Can you make an Eijkman’s test elevated temperature w
I have added it to my list. Thanks for the idea!
I’ve got a video now on the Eijkman Test. Thanks for the idea. ruclips.net/video/WIAaxnyl1yE/видео.html
Why does Coliform bacteria ferment Lactose?
They have the enzymes necessary to carry out that process to generate ATP.
hi, how much lactose broth is in each tube before we adding the sample ?
its actually varies from 31 to 33 31 is more acurate
nice mam
How 33 ?
Thanks but is this methods can be used for soil or it is suited only for water?
This method is suited best for very dilute concentrations of coliforms, meaning not very many present in the sample. There are typically a lot in contaminated soil and so different methods are used instead.
Where does 93 and 11 come from?
Numbers in the MPN table are based on statistics. The table was developed to give best estimates for bacterial cultures.
I hit the like button upon seeing her she's so beautiful!and the video was very helpful and easy to understand.i'm definitely subscribing💙
Ma'am what is your source for this info? Badly needed ASAP. Thank you so much
Just my brain! The MPN method is also covered in the OpenStax microbiology textbook and the Tortora textbook
How did she does to have 33 per 100ml?!🤔
its so because she is really cute....and she can do anything.....
you look for that pattern on the table. 4-3-1 under combination of positives and then 33 under MPN index. you probably don't need this answer a month later, but hey maybe it'll help someone else lol
@@angelicabell9231 you help me! I have a test this morning 😁
@@angelicabell9231 you also help me! Thank you
She’s cuuute.
O bhai . ......moj krdi ......
Sorry, I can’t understand your comment.
Hi I'm Alaa i live in iraq ....can I translate your videos to my language (kurdish) ,and share it in my channel??
Are you just adding closed captions to my video in Kurdish and then sharing the video on your RUclips channel? I think that is fine, but please do not upload a separate copy.
🥰🥰🥰🥰
Educational content disseminated as intended. No begging for likes or subscribes, music to my ears.
Well done on your weight transformation!!!
You are a fucking douche...she is not on here making self tutorials or biographies...asking for how you think she looks...she is a Professor TEACHING MICRO..I can not believe this is how women are openly treated..sickening
Maam you r so beautifull
I m in luv wid u😆
Will u be my bacteria..nd i m ur caliform😊
mam i think u shud wear a mask on ur face,,,,bcz one cannot concentrate on the topic while having your beautiful face in front,,,,
Bruh 🤣
Wow will u marry me😍😍😍😍😍
u lost ur weight,and look fabulous.
Well explained
But your cuteness is distracting me 😉😉
This is very help full video ma'am what is your gmail id or social media I'd...?