Hotstart PCR

[@nadeemkasuri1889]

how can polymerase activate in lower temperature....and how can polymerase bind while our DNA strructure is still double stranded before 95 degree?

[@suvethas1851]

Thank u sir , your lectures means to help me a lot during examination times 😊

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@nutribox4535]

thank You shomus, now i am fully satisfied about this topic after i read the same topic at 24hoursofbiology

[@kayalvizhi8422]

thanks sir,you have explained very clearly

[@shomusbiologyofficial]

You're welcome

[@LordChimi]

Your videos help me alot shomu da...thank u so much!
But please upload some videos on biodiversity

[@aboutstudies123]

Thank you Sir. Easily understood

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@د.محمدبهجت]

Your videos help me alot

[@shomusbiologyofficial]

You're welcome

[@hunaidrangwala3413]

Thank you so much sir....it will help to make my presentation

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@omarahmed-hx6pv]

I have a question please. In the 2nd step, or second way ( modification of primers). How these binding proteins can still normal after the 95C of the denaturation step , until arriving annealing step of 72C that will degrade it ?

[@malikwaseem7043]

ALLAH bless you sir....❤❤

[@buharimuhammad5989]

Born to explain

[@salonipatnaik4677]

Thank you so much, this helped a lot:')

[@DashRJ2000]

Thank you sir 🙏

[@shomusbiologyofficial]

You're welcome

[@krishnazalavadia832]

Thank you sir.... Keep it up

[@salwagamal3484]

Where are the rest of videos of pcr ?
Can you show them as it appear as private videos?

[@amalelhakkey540]

Thank you

[@shomusbiologyofficial]

You're welcome

[@ambikabasu4075]

Sir, after the binding proteins get denatured, the primers are free to form primer dimers , because unless the annealing temp comes they won't bind to the strands! Then how is it helping in preventing the primer diner formation! And moreover at 95 degree the binding proteins will denature, won't the primers denature too? Or are the thermostable like the polymerase?
Sir please answer

[@vijayromeo9013]

thank u sir..

[@shomusbiologyofficial]

You're welcome

[@piyushkumar-sh6mk]

Thanku so much

[@shomusbiologyofficial]

You're welcome

[@sashajaiswal8134]

Please upload BIODIVERSITY AND IT'S CONSERVATION for AIPMT .

[@chandrasekar7087]

Other types of pcr videos are deleted. Please upload them back?

[@prabirsaha688]

sir can you tell me what is asymmetric PCR?

[@zahidmehboob4707]

tnx sir

[@shomusbiologyofficial]

You're welcome

[@suvankardas9550]

Please refer me a good book on Recommend DNA technology

[@arpitadas188]

primrose

[@kanureetkaur624]

i need ur notice reagrding hotstar pcr

[@ritikatyagi8820]

Plz koi to video hindi m bnado