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Tissue Culture Media Development
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- Опубликовано: 15 июн 2024
- In this video, I go way back to my plant tissue culture roots to talk about development of plant tissue culture media and optimization of media for growth. The main lesson for optimization of media for growing your orchids at home or in flasks in the lab is to perform a proper comparison before you change things.
I show part of a large scale replate medium screen that I am using with a bunch of different orchids (ongoing) and a small side-by-side 2 medium screen that I evaluated using ghost orchids (done - with unusually clear results).
Your flasks are fabulous. Over 75 seedlings in a flask. Sensational growth on the seedlings.
I try to get bigger plants, rather than more plants. The runts do not do well on deflasking....
So interesting!! How amazing to create optimized media! I’m excited to hear more about the results. 🎉
Yeah - I do hope that I figure out what is in the banana. If the approach is right, I may even have to change my orchid fertilizer!!...
Very nice! Love these experiments! Can’t wait to start experimenting with making media… at some point in the future!
It is fun - and a little frustrating, if things do not work.... Most results will be negative or hard to see. Occasionally, things do work!
Great video. Thank you for sharing.
Thanks for your comment - will share more later - keep on watching...
Omg,,,ghost orchid❤❤❤ they are so rare ,,,I am trying to grow seedlings from our native tillansias that I have in my garden ,,,
I think that there must be a part of the De Beers family overseeing Ghost Orchids. With the large numbers of seed in each capsule, they should not be so rare but they are.
Do you have giant tillansias - the rare ones?
Hello, great to have a Scientist in our growing orchid community. I am curious of how much we can learn from your knowledge in growing from tissue culture. Be Blessed and My Husband and I just subscribed to your channel.
Thanks for subbing. I think that the composition of my media helps me to understand what type of fertilizer to use on my plants AND I can get my seedlings to grow really quickly on my new medium. I also now know that orchids are special and different in so many ways from the terrestrial plants that I worked with over my career - I think that there may be a new video in there somewhere....??
@@plantpropagator awesome... I know that in my country the Dominican Republic, many growers use plantain water lol. I just use the Fertilizers that have been specifically made for orchids. You may solve the mystery of what's in the banana, beside potassium ext. Keep us updated... how exciting! 👏
I love this! If I hadn't turned out as a visual artist and set designer, I would have almost certainly become a botany lab scientist just like you! I'm too old for becoming a proper scientist at this point, I'm just about to enter my sixth decade of life now... But I do everything I can to make amateur, home setting orchid seed culture a more common and somewhat reliable thing! I keep thinking how abhorrent my methods must sound to an experienced and accomplished scientist like you... lol! I use exclusively what you call Undefined Media Addendum in my formulas... Sounds like a nightmare to you, I suppose... lol. So, you can understand why I'm such a fan of your channel and why I admire your work so much! You certainly have a customer in me, once your media is out in the wild... I mean, out in the market... 😆 Just one question about this: Is it something in the baby formula or is it something in the banana itself??? I use fresh ripe banana in my formula, not baby food.
Thanks for your comment - it is the banana.
@@plantpropagator I use chayote, sweet potato and tomatoes along with the banana! I have no idea of what if anything each of them contributes with... but: Tomatoes are a classic and they are great help adjusting ph. So are the bananas. The sweet potato is my idea, I have heard (yet not from reliable sources) they might have something to boost root growth. And the chayote, also my idea, is just a hunch. Besides being one of the cheapest vegetables where I live, I see that the seeds grow amazingly fast, as they are planted with the fruit still around them. There "could be" something favoring growth in that pulp... all feasible arguments, but absolutely no science behind them!
The topic of how to most efficiently optimize a multi-variant system is actually kind of fascinating when you get to thinking about it. On the subject of revisiting a variable in a multi-variant system like this after you've optimized it once: If you only optimized that one thing while keeping all other variables the same, you almost have to revisit it at some point if you're looking to find optimal performance. It's like driving on a grid in dense fog so you can't see what's around you. You can keep going along a straight line till you reach a high point in the road and then turn 90 degrees and do it again, that doesn't insure you've found the highest point on the grid. You would have to turn 90 degrees again and look for a high point along that axis, repeat till you are satisfied that you aren't making any substantial gains in performance. And that's assuming only one local maximum on the grid.
Sorry, I don't mean to be critical. But like I said, it's sort of a fascinating subject to think about.
I'd love to hear more about the different ingredients in a typical media and their purpose/effect on the seedling growth. I was talking to a local orchid grower here in CR who was telling me that preparing your own media, as in mixing the individual ingredients yourself, wasn't worth it even his small commercial operation, despite prepared media being very expensive. His main concern was the need to be extremely precise with tiny quantities of some of the ingredients, which he felt was beyond his abilities.
I feel like this is akin to the Travelling Salesman Problem, You'd have to be a slime mold to solve it!!! 😄
Media prep can be complex, especially if previously untested components are added. There are papers out on multivariate media analysis and optimization but this is not my area of expertise.I do not think that it is useful to go forward 2 steps and then move backwards, just in case you missed a minimal benefit. I like to learn from the many years of published research and not start from scratch. So, I did start my formulation with a proven orchid medium and make modifications based on what I have learned over the past 45 years, along with some regular optimization experiments. When I do periodic side-by-side experiments comparing the original orchid medium (which is from a medium supplier) with my almost finished improved medium, the results are pretty striking.
My medium will be commercially available one day but the user must understand some of the preparation requirements.
I'm excited for propogator propagation media
after KC, and MS, coming soon: PP 😂
The media prep companies have all said that they would sell my medium - it is just not ready yet. I am hopeful that this recent experiment will finally answer my questions....
@@plantpropagator you don't want to start your own media empire?
@@johngriffin5446 😂😆👍
@@johngriffin5446 Sounds great - kind of like the emperor with the no clothes?
Thanks for the interesting insights!
In mammalian cell culture we have a similar problem with FBS (besides ethical concerns), we just don't know why it works or which of its components are making the media more effective (yet).
I do have other media addenda that are not well defined but I am OK with those. I tried some things that I thought would work and also thought that I was clever but no, I was not.......
Neat!
Thanks!
Can we purchase medium from you?
Sorry - not yet - my medium is not quite optimized -maybe soon??... If you are interested in flasks with orchids, you can contact me at TheOrchidPropagator@gmail.com
Here in India the banana baby food is not available and the ones in the market have lot of other additives. Is it ok to use ripe banana for germination medium. In the past i have used banana for my Phals tissue culture.. Congratulations you are are almost near to developing a novel medium for orchids. I look forward to Sigma selling it soon.
@@mgpurushothama1991 ripe banana is OK but the components of banana change a lot over time. I evaluated ripe bananas that were frozen and then blended, and they worked fine but I do not know if the important components are volatile - I do not think so but I am not sure. One of the things that I am trying now in my screen is banana powder, which a lot of people use.
What about coconut water? I have heard people use that for orchid replate medium.
@@falgunitrieu6955 Sure - coconut water is another one of those undefined media addenda. There are a lot of different compounds in there that could contribute to growth. In the early days of the use of coconut water, it was added since it contained cytokinins, which are hormones that contribute to cell division and growth. These may not be needed and may actually cause proliferation rather than development. Others people will use potato and tomato extracts - and many different fruits will also do. I am trying to develop a medium with defined chemicals.
It makes it even more challenging (for consistency) when different people use fruits of different ripeness or ages. The baby food is going to be fairly consistent but I would like to be able to go to a chemical in the lab and just use that.
Thanks for your comment!