Basics and principle of Fluorescence & Phosphorescence measurement | Learn under 5 min | AI 06
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- Опубликовано: 7 сен 2024
- Analytical Instrumentation - Fluorescence & Phosphorescence measurement
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You may have come across terms like fluorescence and phosphorescence. You may have a lot of questions in your head regarding this topic like: What does fluorescent mean?, How does fluorescence works?, How does fluorescence spectroscopy work?, How does fluorescent occur? You will find answers to all these questions in this video.
In the following video, viewers will learn about the basic principle of fluorescence and phosphorescence. The generation of fluorescent and phosphorescent emissions is discussed in detailed using an energy level diagram of a molecule. In the energy level diagram we can see different levels of molecular energies. The electrons when in pair are either in singlet state or triplet state. The basic definitions of singlet state and triplet state is covered and pairing of electrons and their spins is explained in the animation. Once we understand the energy levels corresponding to singlet and triplet state, it becomes quite clear to understand fluorescence and phosphorescence. The fluorescence and phosphorescence emitted by a sample under test is used to perform qualitative and quantitative analysis on the analyte. It is performed using spectrofluorimeter. The basic principle of measurement of fluorescence is also explained in the video.
At the end of the video, viewer will also have a clear idea between the differences in fluorescence and phosphorescence and will be able to compare the two topics.
Hope you like and enjoy the video. Give us a thumbs up. Stay calm and keep learning. Subscribe to the channel to learn about such more interesting concepts and to never miss any new updates.
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I really like the video but I would like to suggest that you remove the background music. It is distracting!
Thanks Bo Song for your valuable suggestion. We are glad you liked the video. We will try to remove the background noise or atleast reduce it's volume level in our future videos :)
No back ground music is good I like it it effective
Exactly,why the hell is background music there
@@PracticalNinjas Would you please reupload this video without the background music?
Due to certain technical issues we are unable to upload the video without background noise
You're explanation and PPT was awesome 😍
But, Please decrease background volume
I'm unable to concentrate on the concept
How I couldn't find this video earlier. Really amazing and beautiful explaination. Finally I can score and understand at the same time 🙌🙌🙌thank youuu
Man, I'm from Germany. And idk what the hell is going on with our teachers but I understood this topic better in not even 5 minutes than in 2 weeks of school lessons. And your video is in English !!!
Very helpful video, thank you so much sir 🙏🙏🙏😊😊😊
Good explanation.But music is make concantrate difficult.
+cheery berry. Thanks for the feedback. Will try to keep music volume low in the next videos 😃
Thank you for your consideration :)
Hope you enjoy other videos on our channel as well :)
Yes keep the music but little lower :)
Sure ACswe91 :)
The 1st second I was like "music😫", but then I was into the mood,thank you so much, I was having real trouble with singlet and triplet state 🌺
WOW, amazing job, this is exactly what I was looking for. I'm shocked more people are not interested in this topic.
The background music adds so much meaning to this presentation. Perhaps more volume could enhance our understanding.
I think you should add why fluorescence light is higher in wavelength, due to energy being lost to vibration
Higher in wavelength dear please correct
@@gameon4195 Lower in wavelength to the incident light is what I meant
@@gameon4195 Oh wait you're right
@@LadyShaydex thanks 😊
thank you so much for making this video you have explained this phenomenon in a very simple and comprehensive way
Thanks Chitra Keshwani for such a positive feedback. We are glad that you liked the video!!!
thankyou. very well explained. it would me better if no background music.
Thank you +Savita Ct for the support and feedback. The background music in the future videos will be kept to a minimum so that it does not distract the viewers from the main concept :)
Agree with you
@@PracticalNinjas why is it called triplet state instead of doublet which makes more sense since there are two?
@@PracticalNinjas Thanks for the video. I actually liked the background music..Could you tell me who the composer is?
This was great. I needed to remember these concepts in short time. Thanks
Beautifully explained... Thank u for making it quite easy to understand!
simple explaination and i understand the whole thing better!!! THANKYOU
Thanks Safwanah Rahman for such positive words.
Nice and telugu explanation... Is better to understand
Great video, really liked the visuals and it helped me understand it more!
Cool!Thank you so much!
Thank you for explaining Jablonski diagram.
Excellent work. Colorful and creative. Makes the video engaging.
Thanks +Amit Vele.
Awesome!! I can never forget the concept after watching this!!
Glad you liked the video Harshita Bhati :)
Good work. it was really helpful for me. this video cleared my basic concepts!!
Very helpful. Thanks
Very nice discription very good way to explained keep it up
Awesome 🤗🤗🤗
Asome sir u define it very easy to understood 👌👌
Very helpful Thankyou so much
well done! thanks!
very helpful for beginner
useful and easy to understand explain .
thanks
Thank you so much sir
Superb explaination
This explanation is really beneficial ...
awesome music!and explanation
Thankyou for the simplified explanation!
Thank you this helped a lot i have to make a oled from Ru(bpy)32+ and this helped me learn some of the basics
Happy to hear that the video was helpful :)
This was so good thank you. Agree with others about removing the music though.
this video is amazing, thank you vvery much
Thanks for clearing out concepts 😊
Helped me a lot. THANK YOU! Suggestion though, remove the effect on the texts. :)
Thanks a lot for your feedback :)
It was so simple and easy to understand thank u
Best 👍 jazak Allah Khair
Very nicely explained 👍👍.... Thank u so much
Amazing video sir! Thank you so much
Thank you so much❤️❤️
Good explanation
Thanku soooo much sir 🙏🏼🙏🏼🙏🏼🙏🏼🙏🏼
thanks you for your efforts, Indeed.
I appreciate the efforts of making this video with intention to simplify the concept. However, i noticed that you have forgotten to mention several things including the internal conversion, types of molecules that can fluoresce etc. Another thing is that you have wrongly mentioned the lifetime in the singlet excited state as varying from 10^-8 to 10^-4 seconds. These l lifetimes are I few nano seconds only.
really simplified explanation
Good explanation..keep it up
This helped me a lot! Please edit out the music.
The Jablonsky diagram doesn't depict orbital energies, but the energy states of the molecular electronic system as a whole.
You cannot put electrons on this diagram like you would in a molecular orbital diagram.
Basically, every energy level in a Jablonsky Diagram represent its own personal molecular orbital diagram which is omitted.
Perfect explanation. Everything I was looking for
Glad we could help you :)
Nice video sir
Tremendous explanation... Helped me alot
Thank you so much for a very clear and straightforward explanation!
We are glad you liked our video Khawllah Roussi
love the video maker
thank u so much
Beautifully explained!!!
Thanks Sukanya! Glad you liked it.
thank you so much Now I understand
we are glad the video could help you :)
Great content
_Awesome_
supper...b i really like this video
Overall this is a great video. Thank you!
You are amaziiiiiiiiing!!!
awesome
loved itt.....
Perfect
Thanks a lot sir
Glad you liked it
Thanks a million !!!! Nice explanation . Keep it up guys 👍
Thanks Paritosh Sharma for the support :)
@@PracticalNinjas but bro my question is why that Intersystem crossing takes place
Nice video sir. Excellent explanation 👍
understandable.Keep up the good work
Thanks +ketiyala tzudi. Glad you liked it
Gud job ...keep going👍🏼
Thanks +snehitha talla. Glad you liked our work.
music volume down or a more no music would be better, but excellent video and very easily understandable, awesome job.
Thanks +Yasmine Bingei for the feedback :) Will surely try to reduce the volume in the next videos. Glad you liked the video.
does this jumping on and off of energy states mean changing orbitals ? or something different ?
well done thanks.
Thanks +Furkan CAPRAZ. Glad you liked the video.
QUESTIONS :
1. Why triplet states has lower energy than singlet states?
2. What can cause intersystem crossing?
3. What can cause the spin change its direction from S1 to T1, from T1 to S1?
1. The answer might sound weird. But the truth is in Singlet state the repulsion between electrons is higher than in Triplet state. Yep it's confusing. Afterall how can +1/2 & -1/2 charges have more repulsion than two +1/2?
Triplet state provides degenerate orbitals, meaning a larger area to accommodate the electrons. In this space similar species(two +1/2) can exchange energies due to the property of degeneracy. Where as in case of a Singlet state the two electrons are stuffed in a single orbital resulting in high repulsion. Now the most interesting part, all these signs + and - are used to denote the orientation of the spin, not the charge. The charge of the electron is always NEGATIVE. Hence very simply, two negatively charges substances(e-) stuffed in a single orbital is highly repulsive and have more energy that two e- in degenerate orbitals.
2. Intersystem crossing (ISC) is a radiationless process of switching orbitals. It is often undergone by heavy atoms. Due to the presence of degenerate orbitals(T1 state) having lower energy (as discussed earlier) the electrons move from S1 state to T1 state.
3. Bro S1 and T1 are not spin directions. They're energy levels(S1 state and T1 state). And the transition of electrons from S1 to T1 is due to the availability of degenerate orbitals (discussed ealier). The change in spin orientation is necessary. Only if their spins are parallel, i.e. two +1/2 or two -1/2, they can exchange energies, and only if that happens they can be accommodated in the degenerate orbitals.
Thank u
Helped a lot
Nice one thanks
:)
Best video ...
Thanks preeti khobragade
Great explanation, I ave a quert why is excitation of radiation at 90 degree
The isc should happen horizontally, so to a same energy level
From there due to vibrational relaxation it comes to T1
Nice and superb
Thanks a lot.
very well explaind
Thanks Faisal Wighral
great video man
hallo
im big fan
Nice
Voice of narrator is really good
sir please tell why the emission wavelength is greater than the absorbance wavelength ?
Thanks sir
Glad you liked our video :)
Sir,thanks a lot for your video... Can you please tell me , that how will I know , whether a given compound is capable of producing fluroscence or not ?? For example, say acetone... Will it show fluroscence ?
Acetone may fluoresce when a high intensity monochromatic sources are made to fall incidentally. It is difficult to analyse whether all compounds fluoresce or not, since we consider that phenomena generally in the visible spectrum; What if it emits in invisible wavelengths...Hope you know that's why we use UV/Vis spectra, thought many paper suggests they can identify, it has many limitations
Edit: For vibrational energy we use IR
Is it molecular transition?
nce vedio thanku
We are glad you liked our video.
How to Calculate Arbitrary Units and fluorescence intensity?
the video is almost great.. really good explanation! but I couldn't hear a thing. the music in the background is not only unnecessary but really disturbing
superb..
Thanks +Shravan Kumar Muppidi. Glad you liked the video.
Nice video man =) and I din't have troubles with the background music...
actually I found it nice hahah poor little children crying
We are glad you liked the video :)
Good explanation plz avoid music
How does the wavelength increase after
Can u plz upload video on franl condon principle ... Its urgent.. Thank u sir
The channel is majorly dedicated to engineering videos. The Frank-Condon principle is a part of quantum chemistry where we have no expertise. Still we will try to understand and if possible upload a video on it. Thanks again for the approach :)