Fluorescence spectrometer : Factors affecting fluorescence

Why few compounds are only fluorescent? Which factors affect fluorescence? Among the various factors, few are related to structure of the analyte molecule such as structural rigidity, structural planarity, number of rings, ring substitution and types of transitions.
0:00 Introduction
1:10 Which types of compounds are fluorescent?
3:20 Internal conversion
3:35 Factors that affect fluorescence
5:27 Type of transitions
7:06 2. Number of rings
8:18 Structural rigidity
8:55 Structural planarity
9:36 Ring substitution
10:39 Viscosity of solvent
11:16 Temperature & pH
12:18 Oxygen
13:09 Concentration
13:42 Quenching

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