Preparation of MS Media for plant tissue culture (Demonstration video)
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- Опубликовано: 16 янв 2022
- Composition of MS media file: imgur.com/I32r1F9
Note: The media after autoclaving looks yellow in this video. For demonstration purposes, we used a different media afterwards to save time, however, please keep in mind that MS media does not change color like this while you do this experiment in real.
clear explananation thank u
very good video for Beginners. Medam where can I purchase the MS MEDIA.
Can you please make a video on how to prepare mineral salts media liquid and how to prepare stock of micronutrients, also how yo use ph meter acid base and calibration..
Thank you
Sure :)
Thanks ❤
Excellent work
What is the amount of sucrose to be added if it is absent in MS powder ?
30 gm
Chemical biological products like waterpowder
Nice explanation man
If I dissolve all the materials into water and adjust ph than also add agar. But I have no time to autoclave. how many days can keep the media in 4 degree? Or I need to prepare again
It should be fine at 4C for 1 or 2 days
What is stock A %
I was confused on the recipe until I seen this video. Does the Pre-made MS powder have rooting hormone?
no, you add whatever required hormones for what your are growing.
what is the composition of stock B,C,D
I have attached a file in the description of this video. You can find the detailed composition in that. Please do subscribe to my channel. Thank you.
Please kaha melega
What did u add to lower the pH
Naoh
Did you add Agar at the end when it solidifies? Please reply!
The media does not solidify until agar is added as it is the solidifying agent
Or use gellan gum
Autoclave temp for media?
15 psi pressure
How to measure ph
Using a pH meter.
Mam
😊
Why MS medium is brown?
Hello. I have mentioned this in the description. The media shown is some other media. This was only a demonstration.
It depends on the agar you are using. Some looks more brown.