MTT assay | Cell Viability and Cytotoxicity determination using MTT assay
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- Опубликовано: 4 сен 2024
- This video talks about MTT assay and answers following questions like:
How MTT assay is done?
How does MTT assay determine cell viability?
What is MTT assay cancer?
What is MTT assay used for?
What are advantages and disadvantages of MTT assay?
How do you use MTT test?
Why is determining cell viability important?
This was awesome, you took the major concepts and gave a quick overview of how they connect which makes it easier to understand
Thanks a lot Sara for your feedback, please share my channel link with your friends and help me to reach big audience
First of all, thankyou for making this content. It helps me a lot during uni 😭👍🏻 You make it so simple and easy to understand
Please share my channel with your friends and help me to reach big audience..lots of love from India
Ur videos are really a way better and lucid than other youtubers 🧡❤
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waooo unbelievable~you really made this video so quickly.I really appreciate all your effort. Well done sir!
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Don't forget to leave your suggestions
@@animatedbiologywitharpan sure sure,I will try my best to share your working for many people .
This video is really helpful. Thank you for your wonderful presentation, it really helped me in my research study
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Amazing explanation sir.thank you so much sir.
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Great sir.Thank you so much
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@@rajarshibose3318 checkout our techniques in biology playlist.
Honestly this was so much clear and helpful ! thank you very much you are amazing !!
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Such an awesome video..thank you so much💓💓
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Thank u.. clear and very beauty explain
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Bro, amazing animations and explained very well!
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very clear and simple explanation. love it
:)
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Arpan, your videos are incredible, please keep going!
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Thank you so much sir for your time and effort :D a very interesting video and it helps me a lot to do my research in my undergraduate study.
Really glad to hear that my contents helped you 😊
very useful thankyou sir
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Thank you for making this video. it really helps.
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Thank u for such good concepts
Appreciate it
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@@animatedbiologywitharpan ofcourse man I will . Keep up the good content
Nice lecture ,
Sir plzzzzzz explained cell revival,cell subculturing ,cell culture , splitting and passaging etc Also with protocol.
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Cell culture playlist ruclips.net/p/PLKtiwIJ8Q7rpNrZRxzBgoH2Q6oz4bJwg2
Thanx sir for your reply .
Keep it up for making such a nice videos
Plz recommend any good book for cell culture protocol related.
Very well-detailed video!
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We need more of such clips ... very informative and concise
please share with your friends
Thank you for this video.
I need to have cytotoxicity studies done for several samples of mercury sulphide on healthy cell lines of liver and kidney. Can you help me on where this can be done and what may be the approximate cost? Thanks in advance.
Hello! so does MTT and LDH assays both use yellow tetrazolium salts that get converted into formazan? why is formazan produced different colours? is it bc in mtt the tetrazolium salt contains bromide (MTT) and in LDH - iodine (INT)?
That "Hellow!" at the start of your videos is my energy drink these days. Haha!
Ha ha ha ha 😂
Thank you so much Sir... Sir can you please explain the Annexin V fitc experiment
Thanks for your suggestion I will make a video on that
Please make vedio on Oecd guidelines 407, 408, 409, 410 and on Neutral Red assay
Sir can you make a video on cell invasion and migration assay
I will try my best
Nice lecture sir.. Useful 😇
Thanks arpan
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Thank you so much !!!
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I would like to ask if the reduction of tetrazolium takes place in the cytoplasm or in the mitochondria, i.e. the dye also penetrates into the mitochondria
thank you sooo much
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Hi, thanks for the video. Please, I have a question: I will like to ask a question about cell treatment experiment with a test drug for 72 h. In such an experiment, do I have to add my drug once and incubate for 72h then check the cell viability or I have to remove the drug solution and replace with the same drug and concentration after each 24h to avoid wrong readings due to degraded drug in wells or evaporation? Please any other person reading the comments can also reply me if you have the correct answer to my question. Thank you
It depends on bioavailability. If the drug is degraded within a day then it has to be added multiple time.
Thank you very much for taking out time to respond to this my question here. I really appreciate it @animatedbiologywitharpan
thanks for the kind words@@aondohembanege9551
Very nice and easy explanation. Pls let me how to making this slide in ppt?
Thanks for your compliments
Can i use this technique to measure cell viability after autolysis of E. Coli??
thank soo much
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I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
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Thank you so much 😭👍🏻
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Nice video :)
Where are you get the animations (the little pictures)? Is there a website link?
I make it using adobe illustrator
But let me tell you similar graphics are
Available at www.biorender.com
How we can generate standard curve or
how we know that how much cell are viable in or standard?
🤔
what did you put in the control and treatment
Could you please elaborate ? I did not understand
Please tell me cells seedling calculation
Must we wait 2 hours after adding DMSO?
Is it a biochemical test
9985 Dibbert Inlet
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
When drug is added into it
Could you please elaborate
What are the material and chemicals required for this experiment?
Refer to this websote for further details
ruclips.net/video/wJIUmE5RSNo/видео.html
It has really helped me a lot in understanding the test report of my research samples. Please let me know in which stage does the treated sample extract come in contact with the cells
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On which website I will find this information in written ?
www.sigmaaldrich.com/IN/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/cell-counting-and-health-analysis/cell-proliferation-kit-i-mtt
Cancer, apoptosis, metabolic activity of cell- MTT assay
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Hi, I have a question regadring with MTT assay😃
Ask
🙏🙏👏
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Sir can you tell me how i find IC50 value from the standard curve of this assay
Which app did you use?
Powerpoint
@@animatedbiologywitharpan Thankyou so much
Y we call it MTT ?
Because of the dye which is used in this procedure,"Tetrazoleum" which is short form of 3-(4,5-diMethylThiazol2-yl)-2,5-diphenylTetrazoluem bromide simply, MTT
Because Umtiti
Can you tell me what is the function of DMSO?
DMSO is added at the end of the reaction to dissolve the formazan crystals formed from the reaction. Otherwise, you can not properly measure the color density of the reaction. DMSO is added only after incubation with MTT dye.
but DMSO has an antibacterial effect, will this ruin the experiment when using e.coli strains for example ?
can u please share your slides
you can buy it @5$ , please emailis at arpanparichha1994@gmail.com for more details