This was great, I've been looking for "sirt1 mitochondrial biogenesis" for a while now, and I think this has helped. Have you ever come across - Miyason Mitochondria Masker - (should be on google have a look ) ? Ive heard some unbelievable things about it and my partner got great results with it.
Hi, thanks for the video. Please, I have a question: I will like to ask a question about cell treatment experiment with a test drug for 72 h. In such an experiment, do I have to add my drug once and incubate for 72h then check the cell viability or I have to remove the drug solution and replace with the same drug and concentration after each 24h to avoid wrong readings due to degraded drug in wells or evaporation? Please any other person reading the comments can also reply me if you have the correct answer to my question. Thank you
To my understanding it is to lyse the cells to release the formazan produced inside the cells, otherwise we couldn't read the absorbance caused the substance
I know this is too late but I cant help it, some do remove the media because it has a color which may affect the results as MTT is a colorimetric assay.
Some people do not discard the medium and just add DMSO or SDS and make a correction for the absorbance, just leaving some wells with medium and making a subtraction of this value to the rest of the absorbances obtained.
can someone please tell me the same process is for candida species ?? i try same but donot remove medium and add dmso ,,i use mtt with pms solution but did not get the desire color
If working outside the cabinet: • Ensure the laboratory is clean and free from dust and other contaminants. • Use sterile equipment and reagents. • Work quickly and efficiently to minimize exposure to the environment. Consider using a laminar flow hood for additional protection.
Taking my baby steps into research, your videos have helped a lot!
Must be very nice with the multichannel pipetter >_>. I have to use the single ones T_T
I feel you're pain, had to do the same at my university.
lool im the same!!!! and i have to use the 96-wellplate formatting...
This was great, I've been looking for "sirt1 mitochondrial biogenesis" for a while now, and I think this has helped. Have you ever come across - Miyason Mitochondria Masker - (should be on google have a look ) ? Ive heard some unbelievable things about it and my partner got great results with it.
The music is very therapeutic.
Music sure makes everything exciting.
Thanks for the video, I am having trouble plating my cells evenly across the 96-well plate. Does anyone have any tips? Thanks!
Hi, thanks for the video. Please, I have a question: I will like to ask a question about cell treatment experiment with a test drug for 72 h. In such an experiment, do I have to add my drug once and incubate for 72h then check the cell viability or I have to remove the drug solution and replace with the same drug and concentration after each 24h to avoid wrong readings due to degraded drug in wells or evaporation? Please any other person reading the comments can also reply me if you have the correct answer to my question. Thank you
is cross contamination a possibility?
instead of a 96-well plate, what about using h.g. wells for culture?
Do I need to resuspend after adding MTT solution?
Isn't the absorbance supposed to be around 400-450nm for purple?
why do we need to discard medium and add DMSO?
To my understanding it is to lyse the cells to release the formazan produced inside the cells, otherwise we couldn't read the absorbance caused the substance
I know this is too late but I cant help it, some do remove the media because it has a color which may affect the results as MTT is a colorimetric assay.
Some people do not discard the medium and just add DMSO or SDS and make a correction for the absorbance, just leaving some wells with medium and making a subtraction of this value to the rest of the absorbances obtained.
To solubilize the crystals that are formed in cells
We need complete kit for MTT assy can anyone send details of vendor
Very nice videos.. i am very thankful.. Abnova thanks a lot
The music is terrific
Which is the first well ? Alphabet H or A ?
very good video..with nice explaination of steps..
can someone please tell me the same process is for candida species ?? i try same but donot remove medium and add dmso ,,i use mtt with pms solution but did not get the desire color
is any one know in running a MMT cytoxicity assay in addition to prodrug what elas molecules would you test
can i do my mtt not inside the bisafety cabinet?
If working outside the cabinet:
• Ensure the laboratory is clean and free from dust and other contaminants.
• Use sterile equipment and reagents.
• Work quickly and efficiently to minimize exposure to the environment.
Consider using a laminar flow hood for additional protection.
What are the apparatus used here. Would like to buy the same. Can someone list it
Multi-well pipette
why we used DMSO?
DMSO acts as a treatment control for the assay.
Thank you
nice video
Great 👍
very nice
thanks for this jop
Nice👏
#BiologyofDiagrams.. 👍