i modelled a target protein only through the use of modeller 9.22, but now i want to use that protein for docking , but before that i need to do minimization of protein structure, can you tell me how to do it through chimera ?
That is a subject for another video.... In summary: plato.cgl.ucsf.edu/chimera/docs/ContributedSoftware/minimize/minimize.html but remember this is minimization in vacuum...you could solvate your protein and minimize firs for much more reliable results...See Tools > Amber >Solvate...
Nice tutorial! How to save as pdb format?
Very useful tutorial, thanks.
Hi may i ask that how should i save the pdb file with the selected query model?
i modelled a target protein only through the use of modeller 9.22, but now i want to use that protein for docking , but before that i need to do minimization of protein structure, can you tell me how to do it through chimera ?
That is a subject for another video....
In summary: plato.cgl.ucsf.edu/chimera/docs/ContributedSoftware/minimize/minimize.html
but remember this is minimization in vacuum...you could solvate your protein and minimize firs for much more reliable results...See Tools > Amber >Solvate...
Muchas gracias!