Migration Assay
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- Опубликовано: 3 ноя 2010
- ( www.abnova.com ) - The migration assay (also known as the Boyden Chamber Assay) is a commonly used test to study the migratory response of endothelial cells. During this assay, cells are placed on the upper layer of a chamber and a solution containing the test agent is placed below the chamber. Following an incubation period, the cells migrated through the membrane are stained and counted. More videos at Abnova www.abnova.com
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Thanks for the video.
I especially appreciate the comment that follow the video indicating the layer that contain the test agent.
Thanks a bunch
I have never been using this method, but as far I know Boyden chamber has a porous membrane at the bottom where migrated cells "trapped", scrapping only remove non-migrated cells. cmiiw
you wash with PBS+ or PBS - ?
how much volume of giemsa, methanol and formaldhyde is added ?
3,7 % of formaldhyde in PBS- or PBS + ?
it should be noted
THIS VIDEO HELPED A TON!! THANK YOU
is it same procedure when we use crystal violet die? this experiment is used Giemsa stain instead of crystal violet.
Thanks for sharing. It will be very helpful for me while performing the experiment.
Does anyone have the exact reference for Abnova's boyden chamber product?
AWESOME!! It really helped me a lot!!
Thanks for the video, it was very very helpful!
It would be good if you cut the membrane and then mount it on a slide and observe/count cells. Thanks
did anyone count?
Dear Abnova, where could
I found the music in the background? Are there any kinds of playlist?
what is the catalog number of the migration chamber?
great video
Most protocols I've seen suggest scraping off non-migrated cells prior to fixation and staining. However I can see the appeal of performing this step after staining, as you can see if the apical stained cells have been removed. Does anyone know if either method is superior?
Do you mean scratch by scraping?
Do you know how I can count the migrated distance of the cells?
Iraklis Moschonas I think some researches using image J software
How much pore size of the insert?
how to cite this video tutorial
After swabing with cotton how do you know which are migrated cells??why cotton swapping done???plz assist
excellent vidio.
excellent
Thanks!