Transwell Migration Assay | CSU BMB Exploring Biochem

Join Visiting Professor Lubna Tahtamouni as she shows how to walk through a transwell migration assay in order to determine cancer metastasis.
Exploring Biochem is a subcommittee of the CSU BMB IDEA Committee whose goal is to increase research exposure and allow students to opportunity to learn techniques used in the field.
If you have any questions, comments, or concerns please feel free to reach out to us at [e-mail] or visit our website at www.bmb.colostate.edu/

[@deboradasilvabaldivia2277]

Teacher, congratulations on the video, very clear and explanatory.
I would like to suggest to you to make a video with Invasion Essay using Matrigel. Thank you very much in advance.

[@teguhsantoso5248]

Thank you Professor. Very clear explanation for the protocols.

[@eduardoduarte1968]

Thank you so much for sharing this protocol!

[@lorenademoraisribeirosilva5880]

thank you for sharing this protocol, very educative!

[@MedguidewithDr.Mudasirsaeed]

Thankyou it was very informative video 🌹

[@hudanoor6009]

Well explained 👍

[@petersamuelemeka8710]

Thank you for this video, Professor.
You said "clean upper membrane to remove migrated cells", then "fix the cells on lower membrane". How many membranes has the insert?

[@coagulatedsalts4711]

there is one membrane. She is referring to the top of the membrane and the bottom. The top has cells that can migrate to the bottom. When she cleans the upper membrane (the top of the membrane), she is removing cells that haven't migrated out of the top well, at the lower membrane (the bottom of the membrane) there will usually be cells that have migrated if the treatment is unsuccessful. if the treatment is successful, no cells will migrate at all! hope that helps.

[@himanshusarkar9412]

Thankyou it was very nice video 😊

[@LionLion632]

Thanks so much for clear explain. Which staining dye did you use ?

[@avocadosnumber498]

Thanks :)

[@sci_djw7670]

We have this assay working well in our lab with automated quantification of migrated cells 😊

[@algirmaalkhamkhuu3223]

Could you please share your protocol or methodology?