How to isolate plant pathogenic bacteria │Streaking and dilution plate │Practical demonstration

This video is about isolation of plant pathogenic bacteria from diseased leaf. Two methods have been followed in this practical demonstration. Streak method and dilution plate methods have been used to isolate plant pathogenic bacteria.
Hope you will like the video.

[@rakhirathod4075]

Very useful thanks.. Due to locked down we are unable to go to lab and learn the technique..so thanks alot

[@juliuskoome3055]

Thanks you.

[@nouraalsaleh9011]

Why was it recommended to perform a streak method when isolating bacteria from infected leaves?

[@thabisomadikizela381]

What kind of environment should one work under to isolate fungal pathogen?

[@priyata1212]

Sir mujhe Agricultural MICROBIOLOGY padna kya ap pda sakte h for preparation ASRB scientist exam

[@Abhira_khan]

For the sub culture which media we used?

[@muhammadaqibismaeel631]

Same as for the early one, it depends what you are culturing commonly used media are, pda for fungal growth and nutrient agar for bacteria growth

[@komamb2444]

Is it a sterile distilled water?

[@lekhrajsinghbaghel9506]

Sir hindi me bola kare kisan English nhi janta.ap bahut achhi dete ho

[@souravbasu1106]

Why should we take 50%infected portion and 50% healthy portion of the leaf ? If we take 100% of the infected portion..what will be the outcome sir ?

[@dmitryplatonov]

Health looking part is already infected. Higher concentration of pathogen is in healthy part near the death edge. In dead part bacteria might already consume all nutrients and die itself, so border between dead and alive is best.

[@rohnikantmishra2409]

video time 1:05, take 10 sodium hypochloride instead of taking 1percent