Spectral Interferences spectral interferences occur when the absorption signal from the analyte (the element you're measuring) overlaps with signals from other elements in the sample or from the background radiation. This can lead to inaccurate results. Methods to Correct for Spectral Interferences four common methods used to correct for spectral interferences: 1- Two-Line Correction Method Principle: This method uses a second, less sensitive line from the same element as a reference. The assumption is that both lines will be affected similarly by spectral interferences. Procedure: The instrument measures the absorption of both lines. The signal from the less sensitive line is used to correct for interferences on the main line. 2- The Continuum-Source Correction Method Principle: This method uses a continuum source (like a deuterium lamp) to measure the background absorption. The continuum source emits radiation over a broad range of wavelengths. Procedure: The instrument measures the absorption of the analyte line and the background absorption at the same wavelength using the continuum source. The background absorption is then subtracted from the analyte signal to obtain the corrected value. 3- Background Correction Based on the Zeeman Effect Principle: The Zeeman effect is the splitting of atomic lines into multiple components in the presence of a strong magnetic field. Procedure: A magnetic field is applied to the sample, which splits the analyte line. The instrument measures the absorption of the split lines. The absorption of the split lines is used to estimate and correct for the background absorption. 4- Background Correction Based on Source Self-Reversal Principle: This method, also known as Smith-Hieftje background correction, is based on the self-absorption of radiation emitted by the hollow-cathode lamp at high currents. Procedure: The instrument measures the absorption of the analyte line using both low and high lamp currents. The difference in absorption between the two currents is used to estimate and correct for the background absorption. Application - commonly used for the estimation of trace elements in biological fluids like blood. It specifically lists elements like Copper, Nickel, and Zinc as examples. In Summary Spectral interferences can significantly affect the accuracy of AAS and flame photometry measurements. The methods discussed are designed to minimize the impact of these interferences, allowing for more reliable and accurate analysis of trace elements in various samples. Spectral Interferences are same for both AAS and flame photometry
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Spectral Interferences
spectral interferences occur when the absorption signal from the analyte (the element you're measuring) overlaps with signals from other elements in the sample or from the background radiation. This can lead to inaccurate results.
Methods to Correct for Spectral Interferences
four common methods used to correct for spectral interferences:
1- Two-Line Correction Method
Principle: This method uses a second, less sensitive line from the same element as a reference. The assumption is that both lines will be affected similarly by spectral interferences.
Procedure: The instrument measures the absorption of both lines. The signal from the less sensitive line is used to correct for interferences on the main line.
2- The Continuum-Source Correction Method
Principle: This method uses a continuum source (like a deuterium lamp) to measure the background absorption. The continuum source emits radiation over a broad range of wavelengths.
Procedure: The instrument measures the absorption of the analyte line and the background absorption at the same wavelength using the continuum source. The background absorption is then subtracted from the analyte signal to obtain the corrected value.
3- Background Correction Based on the Zeeman Effect
Principle: The Zeeman effect is the splitting of atomic lines into multiple components in the presence of a strong magnetic field.
Procedure: A magnetic field is applied to the sample, which splits the analyte line. The instrument measures the absorption of the split lines. The absorption of the split lines is used to estimate and correct for the background absorption.
4- Background Correction Based on Source Self-Reversal
Principle: This method, also known as Smith-Hieftje background correction, is based on the self-absorption of radiation emitted by the hollow-cathode lamp at high currents.
Procedure: The instrument measures the absorption of the analyte line using both low and high lamp currents. The difference in absorption between the two currents is used to estimate and correct for the background absorption.
Application
- commonly used for the estimation of trace elements in biological fluids like blood. It specifically lists elements like Copper, Nickel, and Zinc as examples.
In Summary
Spectral interferences can significantly affect the accuracy of AAS and flame photometry measurements. The methods discussed are designed to minimize the impact of these interferences, allowing for more reliable and accurate analysis of trace elements in various samples.
Spectral Interferences are same for both AAS and flame photometry
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