Atomic Absorption Spectroscopy | Introduction & instrumentation
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- Опубликовано: 7 мар 2019
- #BaaYo
Atomic absorption spectroscopy is a commonly used technique for the determination of single elements in compounds. As the name suggests, the particles must be atomized in order to perform analysis. After the compound has been atomized (usually by a flame), a radiation source produces waves that pass through the substance and are received by a detector. The most common light sources are hollow cathode lamps, and the most common detectors are photomultiplier tubes.
The technique makes use of the atomic absorption spectrum of a sample in order to assess the concentration of specific analytes within it. It requires standards with known analyte content to establish the relation between the measured absorbance and the analyte concentration and relies therefore on the Beer-Lambert Law.
In short, the electrons of the atoms in the atomizer can be promoted to higher orbitals (excited state) for a short period of time (nanoseconds) by absorbing a defined quantity of energy (radiation of a given wavelength). This amount of energy, i.e., wavelength, is specific to a particular electron transition in a particular element. In general, each wavelength corresponds to only one element, and the width of an absorption line is only of the order of a few picometers (pm), which gives the technique its elemental selectivity. The radiation flux without a sample and with a sample in the atomizer is measured using a detector, and the ratio between the two values (the absorbance) is converted to analyte concentration or mass using the Beer-Lambert Law.
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if the metal i want to detect is unknown, how to produce radiation of a specific wavelength from hollow cathode lamp?
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Ma'am is it useful for detect Plastic contains in food products (Basic in nature pH:9.2-9.6) stored in plastic container?
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whats the main difference between aas and aes as both have same application to detect metals
Can you explain on other techniques of aas like furnace mode, mhs mode
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Do we also use radiation source in AES mam ?
If no, than how e- excites in nebulizer of AES.
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Ma'am I have a doubt. Won't the sodium ions in the sample on reaching the flame get excited and emit the characteristic radiation 589 nm? They don't just absorb radiation but emit too right? So won't the transmittance be more?
what about the radiation emitted by the atoms after coming back to ground state? wont it affect the results?
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Btw I have a question mam.. If I am working in an industry .. I come across a situation where I need to know a certain concentration .... What could be the reason I might go for this method ? Why I would give this method preference over other methods... I am stucked here.. ?
Maam will you please cover the all technique portion for us? All molecular as well immuno techniques from csir net syllabus...it will be very helpful..
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Can you explain the difference between atomic absorption spectroscopy and atomic absorption spectrophotometery ؟?
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Ma'am what is difference between atomic emission and atomic absorption spectrum?
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Does an electron emit the radiation with the same wavelength as it was absorbed?
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mam in flame photometry u said metal of sample get atomise due to high temp of flame so why we use a radiation source in this?????
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AAS.
Temperature sensing and measurement.
Exact and perfect sputtering point measurement.
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Mam can you reply, the above video is same as flame atomic absorption spectrometry. ....or what can you please tell mam