Cheap DIY Mycology Laminar Flow Hood for under $70
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- Опубликовано: 8 сен 2024
- Links to the items discussed in the video can be found here:
ezmushroom.com...
ezmushroom.com...
Hi, I just wanted to share my cheap flow hood creation which I use for mycology and mushroom growing. I use this for agar work, cloning mushrooms and agar to grain work. This video also contains cloning of an Oyster mushroom and Lions Mane mushroom with the subsequent Agar results being discussed at the start.
I apologies if the quality is bad I didn't put the 4K video setting on my phone during recording and this is the first time I have ever done a voice-over.
Good video, but one thing, never cut open the mushroom with the scalpel, the blade going in contaminates the inside, you should always rip it open with your hands or something and then use the scalpel to cut an inner piece.
okay idea,however i dont think you would have a high enough CFM to produce a laminar flow, I will be doing what another commenter suggested which is using an air purifier to get the air thats flowing through your hepa filter already 99.7% clean. Since doing a fair bit of research ive decided that one purifier wont cut it and i will actually be doing two for increased CFM and therefore a better laminar flow. im trying to find a couple with decent flow and the controls in the right outlay for my design. If my flow is too high i can drill holes in my box to reduce flow through the filter or i can adjust the speed settings on the actual machine. In addition to this i will be adding black silicone to the border of the filter to ensure 100% air tightness. Cool video. If anyone is interested i will be making a video of my own in the near future on a new account for mycology.
Happy growing!!
Tonight we are filmin' like it's 1999.
Good video, was expecting an American with the price in $. As pointed out, while this may not be a true laminar flow hood it’s a great starting place for us on a budget. Watching it a couple of times I think that if the fan was in the base of a 2nd box behind the work box and had strips of corrugated cardboard sandwiched with the holes facing front to back it could give a more laminar flow, or a wall of drinking straws, think I saw them do it on mythbusters. Just an idea for anyone else not happy with the laminar content of your flow 😉. Great first attempt, hope to see more 👍
yes a much larger filter with fan built into a room-air unit can be had around $100..four times the flow and much larger work area. Still very reasonable. We see plans to buy filter and fan and wiring but this adds up to 250/300 when you can buy the assembled units from costco or chinese retailers for half the parts cost.
Well done, your much more succesful than me. I've finally given in and bought sterilised grain and a spore syringe from ebay. I'm to impatient to be sterile!! Not listening to the 'that wont work' is your secret weapon. It only counts if they can demonstrate it, not just imagine. Mycology channels do really well.
When it comes to sterile procedure it is a bit of a chore at first then after the first 100+ times it kind becomes like riding a bike :) thanks for the shoutout!
@@ezmushroom I had about 80 more times to get it right!
@ Sean so your laminar flow hood doesn't work. Great I followed your video to make one. However my filter is a better one. Now I'm worried to use it. I guess I'll be working in a still air box in front of it too. :'(
Sorry I meant Shaun.
@@mycomama4209 Hi Virginia, does work, but still to much trouble to pack away and set up everytime for me. I think i spotted a pattern that whatever method you use so long as you gop through the motions of being obssesivelt clean you will have succes. Got several uncle bens rice bags turning white as we speak. I could never be sure that the mycelium syringes were contam free, as they were from the web :)
love the flow hood idea and building one out of a room air purifier, very similar idea. 140 bucks at costco..should work well.
Thanks for the part list, I used a different fan with a higher rpm, but the same filter! Hopefully it works out.
Following
Tell us if it work works
@@thatoneguy9482 Just got it all in the mail the other day. I was very impatient so I inoculated some agar jars in open air and they all look terribly contam'd. Hopefully once I build this lil box it'll be better. Post updates soonish.
@@bigwes9657 be waiting
Soo my own research I've just kind of come to the conclusion that a simple SAB with lots of sterilization is BEST. However; this is a suitable way of creating a sterile environment if you wanted to say, have a safe place to play with your agar and do transfers etc. Worth it? ehh not really in my opinion but I have it now so whatever; I'll use it eventually.
Good idea fellow myconaut.
Thanks lot...👍👍👍👍👍👍👍👍
Came up to my mind to make double filter (side by side) kind of that to have a larger space? Is that possible?
home mycology is so experimental, honestly worth giving it a go. might be worth looking into solutions as to how to make the air flow laminar too
More??
Doesn’t look laminar when he holds the flame
smart mate
Just received 3000rpm noctua 140mm fan today you recommended and no where near enough air flow I have the kaze coming Tuesday so will be able to check further
make sure the fan is right up against the filter, normally my box will start moving so i have to tape it down.
@@ezmushroom tried everything , just massive resistance from the filter, I've bought another filter just incase
@@garrysutcliffe6254 I wedge my computer keyboard at the bottom of my fan so it doesn't blow backwards and tape the box down so the resistance goes through.
@@ezmushroom yes again I've manually held down both with my partner
Sorted it , was a dodgy filter I had , new one works fine with kaze running , glued it to the back of the hepa works a treat thanks
I'm having a lot of trouble believing this. Given the size of the filter, you would need at the very least 100CFM of air flow to push anything through - and you're not even sealing anything. These ventilators usually have less than 10 CFM ... Either your filter setup has a leak, or this is BS.
www.amazon.com/gp/product/B00KFCRATC/ref=as_li_tl?ie=UTF8&camp=1789&creative=9325&creativeASIN=B00KFCRATC&linkCode=as2&tag=ezmushus-20&linkId=a0889cf2c2fcb96c3d2fe0b6f510b65c
How many CFM's does this fan push?
Answer:The fan was designed is Austria ( I doubt it was made there); so the specs are 186.7 cubic meters per hour which is 109.9 CFM.
By Lee Oliver on April 7, 2015
186.7 cubic meter/hour = 109.887471 cubic foot/minute
By Notvis on April 7, 2015
Would a 2,500 rpm fan work?
this can be called a flow hood, specifically because you have an enclosure apparatus. However, this is not laminar flow by any means. Might still be a good tool for amateur mycology though, just need to update the name for a more truthful tagline.
Hiya mate, trying this out in mid 2022...using the noctua fan, running at 3000rpm with 180m3h.
But unfortunately, air will just not pass through the hepa filter. I've tried a different filter of the same brand, in case of a fault, and nope no luck. The air just deflects back off the filter, plain and simple.. Am not sure what to make of this....any ideas?
Cheers mate. Does seem like a brilliant idea for home mycology....would love to work out what's going wrong compared👍
I would place mine really close to the filter, like right up against it then jam my keyboard behind and tape the box down to the fan so it didnt move.
The fridge you have is it an incubator?
Just wondering what are the dimensions for the fan?
How to do the wiring for the pc fan, I'm not electrical savy.
Hi because imà not an electrician i dont want to give that advice out myself. You can get an convertor online OR its a very easy topic to google .
Thanks for you comment and giving this a go!
Your flame test proves the flow isn’t laminar. But if it works and you get no contam, good for you.
Indeed.
I'm going to build one of these. Wsy cool bro. I didn't see the links can i get the filter link?
Link for everything is in the description.
would a leaf blower motor work? it seems powerful and i can get one for 20 bucks used
Sorry for the late reply. YES! Get a bigger box and a big filter too like this.
ruclips.net/video/NDdhRyvpEis/видео.html
@@ezmushroom yes i will try it. thanks
Im sure it is way better than nothing
Waiting for more videos???
have you had continued success with this build?
Yes! I will be making more videos soon including one of me using this at my dirty computer desk :)
Did this actually work?
Can you work with grow bags in there?
Probably, when i do larger jars i extend the box by wrapping turkey bag on the end (tape it and the flow will keep the flexed).
Sorry where did you get your plastic box from? Managed to get filter and same fan as you cheers
All sorted now, I have bought the same fan as you have and filter, just awaiting on the fan.
Meanwhile have a scythe 2600 rpm fan which out puts 110cfm , no air is getting past the filter!
Hopefully the kaze will have another back pressure to force through
@@garrysutcliffe6254 Make sure you are giving the Fan 12v too!
@@ezmushroom I have done , even increased the voltage to 17v fan in turbo nothing getting through the filter too much resistance and fans blowing back , think I have a dodgy filter
@@garrysutcliffe6254 see my other comment about placing a PC keyboard behind the fan so it doesnt move backward (what i do).
Hey man so your using this for agar and all. Since you have been using this has any contam come thru
No, my website in the description shows plenty of clean results.
Cool good to know, just started and tryna stay in a budget so thank you for the reply imma try it out for myself
Also 120mm fan?
Yes just make sure the RPM is very high for flow.
Is it ok to keep the lid off of the bin?
probably not since contaminants can land inside the bin onto your filter - then when you turn it on it'll blow those contams everywhere
Yes its fine, just run the system for like 10 mins before putting anything in, then wipe the box down with aclohol.
@@ezmushroom okay thank you
what percentage of contams are you having using this ?
HI, if you checkout my website in the description i discuss this. I have had very few contamination using this even on my computer desk. Only 2 liquid cultures have contaminated and both times i accidentally knocked the lids. All my clones, transfers etc work fine.
@@ezmushroom wow. that's very impressive indeed. I may have to build one just like it then. thank you for sharing. imma check out yer website soon.
I've seen so many people doing this and failing
Use a sab or a flame if you can't afford a flow hood
Remember just because its growing out clean does not mean its good.
Id assume those people would also have failed while using a SAB or flowhood as well. No device will make up for bad sterile technique.
Actually if it's growing out clean it does mean it's good. Seems to be well demonstrated. There is also obviously enormous prejudice against this sort of option. Prejudice is what it is.
@@douglundy5755 no just because it grows out clean doesn't mean its good. There's a lot more to it.
@@geinstein8407 like what? We know genetics is a factor. But when it comes to contamination clean is what you want. And if the flow Hood protect from contamination... what more do you want it to do?
@@douglundy5755 Well firstly, there is Contam that won't ever show on normal dishes, how ever would lead it Contam later on grain sub extra
Most of the time these are bacterial contams, your dish would look clean because these won't grow on most media we mycologists use, some better places with more resources would use recipes where only these contams would grow to double check, and some even use PCR machines.
Next are similar, how ever you can pick them up with a trained eye. These are fungal & bacterial contams that would ride/grow with the mycelium making it look as if you've only got mycelium is completely clean.
Next genetics, there's a lot more here then just what a mushroom does
You can have two identical genetics from the same dish, and have them preform differently, most of the time handling during culture work is responsible for this and is another reason people use Flow hoods or flames to make the work easier.
all the facebook experts assure me this doesnt work, your DIY affair but Im ordering a much bigger unit for room air with a 16/12 inch filter and four speed fan to build a bench flow hood, its a great idea!
Did your version work out? I've tried this myself but in another configuration. I did a lot of testing and found a very high rate of contamination.
@@carlfranz sorry replying by phone and I didn't understand what the comment regarded. Yes my flow Hood dealt with a room air purifier seems to work and gives a good flow rate. I do struggle with contamination and it's mostly because I live on a sailboat in a coastal climate with lots of high-wind.
yes, works fine and yet there are lots of aspects to consider..These discussions arent clear enough to be useful and one can see so much prejudicial opinion I have very little respect for the conversation although I see good ideas shared in the videos.
No music is distracting ! Anoying !