HPLC - HIGH PERFORMANCE LIQUID CHROMATOGRAPHY | PHARMACEUTICAL CONCEPT | PC [2021]

#PharmaGyan HPLC - HIGH PERFORMANCE LIQUID CHROMATOGRAPHY | PHARMACEUTICAL CONCEPT | PC [2021]
In this video we will discuss about HPLC - HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
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🌐 HPLC chromatography lecture - This lecture explains about the HPLC chromatography technique in a nutshell by Guru Balaji. HPLC is performed to separate organic and biological compounds using solid stationary phase.
🌐 High Performance Liquid Chromatography (HPLC) is a form of column chromatography that pumps a sample mixture or analyte in a solvent which is known as the mobile phase at high pressure through a column with chromatographic packing material known as stationary phase. The sample is carried by a moving carrier gas stream of helium or nitrogen. HPLC has the ability to separate, and identify compounds that are present in any sample that can be dissolved in a liquid in trace concentrations as low as parts per trillion. Because of this versatility, HPLC is used in a variety of industrial and scientific applications
🌐 Sample retention time will vary depending on the interaction between the stationary phase, the molecules being analyzed, and the solvent, or solvents used. As the sample passes through the column it interacts between the two phases at different rate, primarily due to different polarities in the analytes. Analytes that have the least amount of interaction with the stationary phase or the most amount of interaction with the mobile phase will exit the column faster.
This lecture explains the following things about Hplc chromatography -
1. Hplc chromatography principle
2. Hplc chromatography instrumentation
3. Hplc chromatography types
4. HPLC chromatography
5. HPLC instrumentation
6. HPLC simple animation
frequently asked interview questions and answers of HPLC, qc interview questions and answers, important interview questions
👉 🕐🕐TIME STAMP🕐🕐 :
0:07 What is HPLC?
0:26 HPLC Principle
0:39 Uses of HPLC
1:14 Stationary Phase / Mobile Phase
1:32 HPLC flowchart
1:54 HPLC Chromatogram
2:31 What is HPLC column ?
3:26 Subscribe Pharmaceutical Concept
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Pharmaceutical Concept
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