Ligation with T4 DNA Ligase

An overview of DNA ligation, followed by a demonstration of the ligation of 2 DNA fragments with T4 DNA Ligase for cloning purposes.

[@StandardName562]

If you would use a PCR thermocycler, why not set the temp to 4 degrees and then up to 16, and then cycle down again for a few cycles? Wouldnt that give the best results..?

[@tiamheydari7729]

Thanks for the great video. Would you please share the reference for the formula?

[@reubennjue7848]

My DNA is in TE buffer..Must I use water in my ligation reaction?