Designing PCR Primers for Restriction Enzyme-mediated Cloning

How to design primers that will amplify a promoter from the human genome, which you can then clone into an expression plasmid using restriction enzymes and a DNA ligase.

[@danieltito1234]

Ok man your video was cool, but i was expecting more about primer design for restriction sites.
I came up on this video looking for the difference between linkers and Oligos designed to add restriction sites of our interest's insert, and to be honest i didn't find it in your video. Good quality tough. Regards

[@cacevedo07]

This Man should have a million subscribers!
Many thanks for your videos.

[@adwoabiotech]

Exceptional walk through - thank you⭐⭐⭐⭐⭐

[@nadaj.9943]

That was really helpful, thanks!!

[@Milo-tl9oq]

you are awesome. Thanks for your time!

[@elissaalam6724]

That was great, thanks!

[@lolohsn2515]

Good video Dr. elmer ! but how did we design primers with R.E sites for new promotor

[@aryarezayi8622]

Thank you ❤

[@rahelehroudi7379]

My aim is to replace the EF1-A promoter in the pT3-EF1α plasmid with CLEC4F promoter. Please guide me in this regard.