Designing PCR Primers

Overview of PCR, followed by guidelines on designing effective PCR primers for the amplification of a target sequence from the genome. Also included is a brief tutorial of Primer3.

[@shannonwright4203]

You just taught me more about designing PCR primers in an hour than my molecular bio professor has taught me all semester. Thank you sir!!

[@polarisgemini52]

Just designed my first primers with restriction sites in them for theoretical work using this video as a guide. This was very helpful.

[@DODYBILLHAQ]

Really great educational video

[@SarkarSAziz]

WOOWW, alot of learning. Highly appreciate it. many thanks

[@lona6061]

Please keep on posting videos like this for the researchers! God bless you! Very well explanation!!

[@islamadel5800]

Organized and easy to understand explanation, Keep it up, Thank you

[@agnesq9338]

Thank you so much for making these videos…saved me on my first job!

[@muhammadwaqasluqman2977]

Absolutely Amazing. Thank you so Much for this.

[@amonoracheal9118]

very clear and enjoyable to follow:) Thank you:)

[@mm669]

Please keep posting these videos. They are so good. Can you do one on antibodies?

[@bio366geethasankar7]

🙏

[@PharmaniaTubemedical]

Thank u so much

[@bryanwang1677]

U r awesome

[@miguelesparzaarmenta2992]

What happen if I loose my TATA box region when designing the primers? Does the promoter still transcribe?

[@shaktiprakash8567]

bro How to add or remove Stop codon

[@anchalchauhan4734]

Please help me design chip primers 🙄