How to achieve fast and efficient ligation.

lifetechnologies.com/rapidDNAligation
How to video to demonstrate how to achieve fast and efficient ligation as part of a cloning workflow using the Thermo Scientific Rapid DNA Ligation Kit .

[@qiannipeng1005]

Will using more ligation mixture such as 20 uL for transformation be toxic to competent cells?

[@thermofisher]

Cells toxicity will depend on other transformation factors in addition to the ligation mixture.
For additional technical support questions, please contact us at thermofisher.com/askaquestion. Thank you!

[@sadaftagar220]

from where did u use linearized vector? is it included in the kit plz inform

[@thermofisher]

Hello Sadaftagar220, Thank you for your question. Please contact our technical support team at thermofisher.com/askaquestion for additional information. Thank you!