This is a really good question! At the beginning I just put them in randomly in relationship to the sizes that I know are present. Remember that the precise positions do no matter - rather it is about where the restriction enzyme recognition sites (cut marks) are in relation to each other. I hope that this helps.
Omg thank you so much, your video is the only video that covers mapping of 3 enzymes, and you explained it so well!!!!
not complicated at all, on the contrary, you made such a beautiful explanation, thank you so so much !
You are actually such a life saver 😭😭😭
You don't know how big of a help you did to me with this explantion......triple digestion was eating up my brain😭
Thank you!! You're life saver ❤️
I LOVE YOU OMG I SPENT 1 HOUR DOING THIS STUPID QUESTION TILL I SAW UR VIDEO THANK U SO MUCH!!!!!!!!!!!!!!!!
Thanku lani 👌👌alots off love from my side 💗💗💗
Thanks a lot miss
amazing video covering mappin of 3 enzymes
You didn't explain the bam + ecor 1 part. You did t explain why you did anyof that
Saying u wont give this for your test when I got it in my exams(very different clg ).
When you do calculation 1.6+0.75=2.35, how can you know that hindi can not be on that side? Thank you.
As the 2.35 is the fragment of Bam alone
This was extremely helpful with my homework! thank you :)
Glad it was helpful!
Thank you :) :)
I can't locate the 1kb fragment between Hind III and EcoRI. The 1kb you mentioned reflects between the two EcoRI?
Thank you Lani ❤️
You are very welcome!
How do you know where to place the cut marks?
This is a really good question! At the beginning I just put them in randomly in relationship to the sizes that I know are present. Remember that the precise positions do no matter - rather it is about where the restriction enzyme recognition sites (cut marks) are in relation to each other. I hope that this helps.
Hi! Could you please explain why you didn't position cuts in the 1.6kb region in the final plasmid?