Sequencing: Plant Wall Heteroxylans Using Enzymic, Chemical & Physical Techniques l Protocol Preview

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Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques - a 2 minute Preview of the Experimental Protocol
Sunil Ratnayake, Kristina Ford, Antony Bacic
University of Melbourne, ARC Centre of Excellence in Plant Cell Walls, School of BioSciences;
This protocol describes the specific techniques used for the structural characterization of reducing end (RE) and internal region glycosyl sequence(s) of heteroxylans by tagging the RE with 2 aminobenzamide prior to enzymatic (endoxylanase) hydrolysis and then analysis of the resultant oligosaccharides using mass spectrometry (MS) and nuclear magnetic resonance (NMR).
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