Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

This is a video protocol for generating genomic deletions in mammalian cells lines using CRISPR/Cas9.
Bauer, D. E., Canver, M. C., Orkin, S. H. Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9. J. Vis. Exp. (95), e52118, doi:10.3791/52118 (2015).