I have made a home scale very small Bioreactor for growing up yeast cultures of Kveik Yeast for drying and freezing yeast slurry , Its a 1 quart to 1/2 gallon reactor depending on which jar i use. Basically its a ball airlock lid( the kind for fermenting vegetables with a built in airlock) that i drilled a second hole in and put a stainless steel air stone that is hooked to an air pump and it sits on a Stir plate, so, if my understanding of the process is correct this would be a Very Basic Bioreactor. My issue is the feeding schedule for optimum yeast growth and how much sugar and nutrient i should be feeding it daily. I have Fermax yeast Nutrient and I am doing my Grow up with white Table sugar because its a cheap easy sugar source. I am building up a personal yeast bank of Kveik yeast strains for use in Home wine,beer and mead making. So My goal is the highest biomass I can achieve with the limits of my home made set up. My reactor it self is working so far I am just not sure on a rough estimate of how much sugar and nutrient to feed it daily. I dont have a super accurate Gram scale(yet) so I am using measuring spoons and cups at this point At the present time, I am doing roughly 1/2 teaspoon of yeast nutrient and about 1/4 cup of sugar daily in a 1 quart volume of water I was wondering how these amounts look and if i should change them, if so how much of each would you suggest. The yeast nutrient I have is a General purpose nutrient designed for home wine,beer and mead making. My sugar source is currently white table sugar due to price and ease of access. Any suggestions you can offer would be greatly appreciated.
I got my data for the lab i did, the problem is the substrate concentration (glucose) decreases over time while specific growth rate increases. I know glucose will decrease as the microbe uses glucose. When plotting the graph, it looks really bad as specific growth rate increases but substrate concentration decreases. What did i do wrong?
Thank you, George. VERY HELPFUL!!!!!!
Thanks
I have made a home scale very small Bioreactor for growing up yeast cultures of Kveik Yeast for drying and freezing yeast slurry , Its a 1 quart to 1/2 gallon reactor depending on which jar i use.
Basically its a ball airlock lid( the kind for fermenting vegetables with a built in airlock) that i drilled a second hole in and put a stainless steel air stone that is hooked to an air pump and it sits on a Stir plate, so, if my understanding of the process is correct this would be a Very Basic Bioreactor.
My issue is the feeding schedule for optimum yeast growth and how much sugar and nutrient i should be feeding it daily. I have Fermax yeast Nutrient and I am doing my Grow up with white Table sugar because its a cheap easy sugar source.
I am building up a personal yeast bank of Kveik yeast strains for use in Home wine,beer and mead making. So My goal is the highest biomass I can achieve with the limits of my home made set up.
My reactor it self is working so far I am just not sure on a rough estimate of how much sugar and nutrient to feed it daily. I dont have a super accurate Gram scale(yet) so I am using measuring spoons and cups at this point
At the present time, I am doing roughly 1/2 teaspoon of yeast nutrient and about 1/4 cup of sugar daily in a 1 quart volume of water I was wondering how these amounts look and if i should change them, if so how much of each would you suggest. The yeast nutrient I have is a General purpose nutrient designed for home wine,beer and mead making. My sugar source is currently white table sugar due to price and ease of access. Any suggestions you can offer would be greatly appreciated.
Thank you sir, for the explanation.
I got my data for the lab i did, the problem is the substrate concentration (glucose) decreases over time while specific growth rate increases. I know glucose will decrease as the microbe uses glucose. When plotting the graph, it looks really bad as specific growth rate increases but substrate concentration decreases. What did i do wrong?
Thank you. Its really helpful
Helpful
Thanks
Sir what is limiting substrate???... Is it control the metabolism of other substrate in catabolite repression?
I think this is the substrate that the micro organisms depend on greatly for reproduction