Splicing

RNA Splicing | group 1 introns | group 2 introns | self splicing | spliceosome |
The transcription of eukaryotic gene results in formation of pre-mRNA. The pre mRNA have coding sequences and non coding sequences. The coding sequences are known as the exons, while the non coding sequence are known as the introns. splicing is a phenomonon in which the introns are removed and the exons are joined togeather to form mature mRNA. the basic mechanism of splicing is simple. the phosphodiester bond between exon and intron at 5' splice site is first broken, then the 3' hydroxyl group of the 5' exon, reacts with the 3' splice site to complete the reaction.

[@sarvsoltani1674]

it helped me in so many ways to better understanding how exactly the splicing takes place! thanks for the great job.

[@ummeabiha7323]

this is so far the most simplified yet the best video on the topic!!

[@CancerEnthusiast]

Loved it! I never understood splicing until now. Keep it up my friend, you have an excellent way of explaining.

[@ummulbanin9845]

Thank you sir for such a simple yet completely clear and understandable lecture

[@okankilic2297]

To add a minor revision, you could substitute "dash" with "prime" word. Thank you !

[@lorrainelolah6841]

hello Okan! can i kindly have any questions on pre- mRNA that you might be studying. i actually approached you since your comment is the most recent.

[@BioTechNomad]

😂😂😂😂 i was thinking the same

[@varnamohan2629]

We use prime

[@rachl8400]

Great video! Super helpful, one thing to note is I believe the correct naming of the G and A nucleosides is "guanosine" and "adenosine" because they are joined with ribose, not on their own.

[@memanjoo005]

Best video on splicing

[@shamshassan6827]

thank you, you made such a complicated
process easy and clear to understand

[@yarelisrivas-avila7610]

Excellent explanation. Thank you so much!

[@SabrinaScience8]

Thank you so much for this video! I'm a visual and an auditory learner and I thought your explanation was perfect!

[@HadasLL]

I'm so happy I found you. You are great!

[@apekshadandi7767]

It was quiet effortless to learn splicing from your video. Thanks a ton.

[@joyganguly6834]

Really thank you for the video sir, I have never understood Splicing this good before

[@paige9274]

great video, explained very clearly thank you

[@bracken7794]

Great explanation, thank you!

[@Sunshine-Muskan]

Great work 👍 very clearly explained

[@rajuislam2490]

Thanks man.. I really get understand by your video a day before my exam.i cann't speak out how am i feeling now!!!!!!

[@chloesmith2607]

Thanks man, I really appreciate this. Have a great day!

[@KarthikDisley]

simplest and shortest explanation of all, very helpful

[@apurbamukherjee794]

It's very helpful, thank you for this video

[@sharonbhambal2084]

Now I clearly understand this concept thanks

[@eisenj21188]

Great video buddy. Thanks!

[@razanfeki2909]

you explained something in 7 minutes that my professor couldn't in and hour and a half !!!!

[@aaronmbwabi]

The best explanation ever!

[@md.ashrafulkarim6778]

very short and to the point..thank u

[@neethugopal2351]

Thank you.. its very helpful and easy to understand.

[@user-me2uu4gy8w]

You are a god send. thank you.

[@akashsahu6856]

Thank you for your help 🙏🙏

[@linagabriel6356]

Thank you so the video.. It was very educating

[@mmmpr2261]

Bless you,man!saved my day'

[@liutianchanzwiers3706]

Thank you so much!

[@lordhelmchen6539]

Very helpful. The simple explanation of attack here and there from my professor was not cutting it.

[@leyiwang3863]

Very helpful!!! Thank you!!

[@mobarkalwahishi3323]

Thank you so much

[@ganeshgr616]

Great explanation..and . good vedio too.......

[@lucyxiong7301]

thanks for your help! you're pretty awesome!

[@danielolumese9083]

thanks making this video mate !

[@Reah850]

I know I will never ever e-ever give you uuup, and I wanna say thank you in case I don't thank you enouuugh...

[@salmasanad710]

thaaabk you so much i spent three days trying to understand this, fortunately i found this and i understood it quicklyy so now i wish if i have founded this 3 days ago hahaha

[@josephineobi8089]

Thank you for the video.
Please can you help with an explanation why in-vitro splicing reconstituted with U-snRNPs is slower than in-vivo splicing?
Thank you.

[@joshmemarrynagdey1639]

Helpful video. Thank you

[@drajiv1]

Nicely explained 👍

[@the_explorer_anje8243]

Thanku so much for complete explanation

[@pakeezahashraf197]

I did'nt understand my teacher's wording
But this vedio help me and make me to understand splicing easily
I just love thiss❤

[@arshbir1539]

Thank you

[@amnarehman1278]

Waaaaaaao 👍

[@khoinguyen0210]

Thank you very easy to understand

[@sujankumar3620]

Super explanation 👍

[@mawadaa.nasser651]

Very simple, Thanks

[@prastutidebi8034]

Really helpful thank you so much

[@rajkishoredas936]

Thank you sir , the video was easy to understand .

[@shaharbanupp6975]

Tysm,, u really helped me a lot

[@bharatidas2108]

Thank you🙏

[@yashusingh2289]

Really helpful..... thank U so much....keep it up......!!❤🤗

[@ayshamalik2963]

Much helpful💜💜💜

[@Suche13]

thank you so much

[@muhammadtouseeftahir9477]

thank you so much❣

[@alfredomunoz8791]

Also Prokaryotes can't have introns, because they have transcription coupled to translation. They don't have time/space for that, since intron splicing will stop the coupling. Eukaryotes evolved the nucleus, where splicing can be done

[@ganmeii8912]

they still can have introns, prokaryotes perform self splicing which means it won’t impact translation time wise but it can still happen

[@mernas1418]

GOATED.

[@niks8924]

Very well explained sir

[@vaishallychugh8577]

Really very nice explaination

[@mohanaburman8467]

Loved it ....

[@asmita_1999]

Great video

[@Wilderness5283]

Thankk you, it is very informative!

[@Mostafa-hz4kj]

Thanks

[@richardshane456]

ty

[@silva0003]

Why are there no videos of examples using a template strand to help us identify introns and exons

[@user-mh3mn9ge2b]

You are great bro 👍

[@anjanasaji6557]

Very nyz clz...easy to study😍❤️

[@funnyvideomarwadi]

Nice information of RNA splicing

[@karansahare1349]

Thanks sir

[@user-tf2vh3jm1x]

1:50 ✍️ It helped me a lot, thx🙏

[@extatickiddo5714]

I’ve never heard prime read as dash lol

[@gretabecker2946]

Me neither. Came to the comment section just to see if that's a thing... So, was this by accident or is it, indeed, called 5/3 dash in some other parts of the world?

[@shubhangimorale9684]

@@gretabecker2946 some people call it dash instead of prime which is conceptually wrong. It should always be 5’prime splice site.

[@nadiatumayine7341]

Thank you for letting me know that the dash meant prime, I was on my way to closing the video because I was even more confused :)

[@londonjeremiah8434]

instablaster...

[@bookishmahsa6634]

cool then I'm not alone

[@manjusamyuktha2013]

Good explanation thank you sir

[@manibio2948]

Thanku so much ........

[@valentinapaolillo6310]

thanks for the help, great video.

[@user8582]

AMAZING

[@ishanbharti8797]

U5 is attached to both the exons, holding them together

[@HarshitKumarSinghAnine]

Thank❤❤❤❤❤❤

[@durgamthirupathi1838]

I love you sir thanks

[@ahmadansari6829]

Great sir

[@deebos980]

wow nice

[@Alkimachos]

am I the only one who thinks snurp is the cutest name ever?

[@manikhalder3520]

loveeeeee you a lottttt god bless youuuuuu

[@sumanpanigrahi7275]

Nice...

[@mitchellhayes2400]

Great video! What is your primary source for this video? Thanks.

[@240_mandakinisabar4]

Tq so much sir i really appreciate you ❤️

[@zoom-zip3473]

So where does the guanine nucleotide in type 1 introns come from?

[@subhasishbhattacharjee8596]

Thank you mam

[@manikhalder3520]

you are gooooooood

[@Prettiest_Aayat]

Oh great

[@ankitcreations9678]

💓

[@syafianursyifa5183]

excuse me, Mr. what's book you were used in this video ?

[@beatrizvelazquez5430]

Great, just one comment, it's not 5 or 3 "slash" but "prime" (like Amazon, ok bad joke)

[@saimsaim8580]

😒😂😂😂

[@kavyashree8069]

He said 3 dash and 5 dash and not "slash". In most of the colleges and universities they teach us like that

[@anshikajain7556]

It's pronounced prime not slash in biology

[@c0rtikoZteroids1]

@@anshikajain7556 It's common nomenclature; "dash" and "prime" are both accepted.

[@anshikajain7556]

@@c0rtikoZteroids1 ik but i was talking about slash. Thanks btw

[@akashbaburaj2349]

can somebody explain what is branching sequence in mRNA and why it is called as branching sequence

[@user-sy6jx7cw7m]

То чувство, когда говоришь по-русски а слушаешь по-английски и все понимаешь)

[@taniabhadra9313]

What happened to the introns after slicing?

[@mimineelam1671]

so lariat forms only on group 2?

[@mashedtoilet3033]

Is "five dash" the correct pronunciation of 5' ? 10yrs ago when I had taken courses in biochemistry and ever since then I've only heard it as "five prime". your video is the first time I've ever heard of "five dash".

[@muhammadabdullah4121]

Why the splicing of intron in transcription occur in eukaryotic not in prokaryotes?