Thank you for such clear explanation of such complex process. 95% of the genes are alternatively spliced -- that is a shocking and very useful information.
Hi professor! I found your video helpful but I do have a question. If alternative splicing gives different sequences how can that be a good thing? I understand that it does bring up diversity but isn't the whole point of making the RNA in the first place to have the code to make specific proteins? How can alternative splicing be helpful when it codes for different proteins? Isn't this close to a mutation? I am just confused about this being a good thing. Thank you for your time!
Hi there! It can be a good thing because it can result in more than one functional protein. Via alternative splicing, it is possible to make different proteins that are similar enough that they carry out related functions that are just a little different from each other. For example, the Drosophila Down syndrome cell adhesion molecule (Dscam) gene has 95 exons. Drosophila can make 38,016 different isoforms by the alternative splicing of these exons. These different isoforms will all have slightly different activity in the cell. That is a lot of variety! You can see some more examples here: en.wikipedia.org/wiki/Alternative_splicing#Examples
Alternative splicing allows eukaryotic cells (including human cells) to use the same GENE to get more than one PROTEIN, just based on which exons are kept in the mature mRNA transcript and why are spliced out. In other words, there can be multiple similar/related proteins possible from one gene.
Yes! It doesn’t alter genes directly, but Alter’d their expression, which creates differences that can be acted on by natural selection. More here: news.mit.edu/2012/rna-splicing-species-difference-1220
Yes, multiple RNA transcripts can be made from a gene and exist simultaneously in the cell, with some being spliced one way and others being spliced a different way.
Please invest in a wearable microphone. Otherwise really good video! Also good with the short selected theory on the left side of the board. Thanks again!
I got it the number from here: pubmed.ncbi.nlm.nih.gov/18978789-deep-surveying-of-alternative-splicing-complexity-in-the-human-transcriptome-by-high-throughput-sequencing/ It is from 2008, so could be out of date; but I think it is still definitely higher than 59%
U teaches really well..i am become a huge fan of u..can i get ur email or number mam??..actually i want to shootout some problem related to genomics..thats why i want this.if u dont mind so could u??
![Seungmin "그렇게, 천천히, 우리(As we are)" | [Stray Kids : SKZ-PLAYER]](http://i.ytimg.com/vi/kAzmhLHePqU/mqdefault.jpg)
That was a very clear, helpful and useful lecture. Thanks a lot from Turkey!
You are very welcome!
Best faculty I ever found to make things easy. Thanks!
Very good and simple explanation.
Greetings from Germany
Yeah
I also was shocked when I first read about alternative splicing. This is an amazing mechanism
Nice video! It is the first one I've found that explains the regulatory mechanism, and not only what it is alternative splicing. Thank you :)
Yea
Beautifully explained and illustrated keep up that great work! Thank you for your time!
Thank u miss, Ur all videos are really simple explained and helps a lot in my studies☺️❤️💕
that was so helpful thank you from Canada!
Lecture was amazing, thank you for making it simple to understand.
Very good explanation, Greetings from Kashmir
Thank you very much!
I LOVE THIS WOMAN! THANK YOU! life saver!
Thank you very much mam
It was a great video and very good information. Thank you.
This is very much appreciated, thank you. Adel from Egypt.
Your explanation was fabulous ♥️❤️ Thank you very much 🙏🏻👍🏻🌹
You're welcome 😊
Thank you for such clear explanation of such complex process. 95% of the genes are alternatively spliced -- that is a shocking and very useful information.
Thank you for such a clear explanation, helped me a lot. ❤
Thanks for the class. Great.
Nice explanation. Greetings from Japan.
This was so simple and informative!
Super helpful and clear, thanks!
Very helpful! Greetings from Germany
Very helpful indeed. Thanks from Brazil!
concise n helpful, thanks a bunch
Awesome. Thank you very much!
god is a great engineer
Can you please tell me from where You bought the non-linear slip watch. I want to buy it please ❤
Please also make a video lecture on the generation non-coding RNAs from TE. Thanks!
omg this helped me so much!! amazing video
Thank you. Very helpful.
Very good explanation, thank you
Greetings from Perú
My best friend’s husband is from Peru. Greetings back to you. :)
@@BiologyProfessor nice!! tell him "que chevere" :D
Excellent..
nice explanation mam...love from india
Lecture is well made. Thanks a lot!!
very good explaind ! Thanks
Very helpful, thank you!
Thank you professor :)
You are very welcome 🤗
This is so helpful, thank you
Thank you very much great explaination make the topic very interesting thank you
thank you very much that was a great video!
excellent video!
That was helpful, thanks!
great video! thank you!
What about alfa-tropomiosyne? Is there any difference in the splicing process between exons 2 and 3 and 7 and 8?
Very good!
Hi professor! I found your video helpful but I do have a question. If alternative splicing gives different sequences how can that be a good thing? I understand that it does bring up diversity but isn't the whole point of making the RNA in the first place to have the code to make specific proteins? How can alternative splicing be helpful when it codes for different proteins? Isn't this close to a mutation? I am just confused about this being a good thing. Thank you for your time!
Hi there! It can be a good thing because it can result in more than one functional protein. Via alternative splicing, it is possible to make different proteins that are similar enough that they carry out related functions that are just a little different from each other. For example, the Drosophila Down syndrome cell adhesion molecule (Dscam) gene has 95 exons. Drosophila can make 38,016 different isoforms by the alternative splicing of these exons. These different isoforms will all have slightly different activity in the cell. That is a lot of variety! You can see some more examples here: en.wikipedia.org/wiki/Alternative_splicing#Examples
Thank you!
What's the importance of Alternative Splicing?
Alternative splicing allows eukaryotic cells (including human cells) to use the same GENE to get more than one PROTEIN, just based on which exons are kept in the mature mRNA transcript and why are spliced out. In other words, there can be multiple similar/related proteins possible from one gene.
Thank you! 😄
Does alternative splicing create genetic variation and evolution?
Yes! It doesn’t alter genes directly, but Alter’d their expression, which creates differences that can be acted on by natural selection. More here: news.mit.edu/2012/rna-splicing-species-difference-1220
thanks!
Is it possible to have 2 or more transcripts for different proteins simultaneously from a single gene?
Yes, multiple RNA transcripts can be made from a gene and exist simultaneously in the cell, with some being spliced one way and others being spliced a different way.
Thank you so much 💓 too helpful
Very helpful thnkuu😍
I just want to learn about splice donor/acceptor sites and where they can be found!
Thank you thank you you saved me
Bravoo!!
please made assignment on Differential RNA Processing
Differential RNA Processing is another name for Alternative Splicing.
Love From India 🇮🇳
Good videos
Thanks! 😀
Brilliant
Thank you very much! You look like emilia Clarke by the way
Just FYI; you cannot skip the 1st or the last exon
Sorry but can you tell me the reason
What can be removed from pre-mRNA during alternative splicing?
introns are spliced out, and sometimes part or all of certain exons that would be left in the mRNA in alternative forms.
very helpful！
Thank you for explaining AS generation mechanism in a good and simple way. I am also looking for the AS analysis pipeline using the Linux platform.
I’m sorry, I don’t know much about Linux. I used Unix for a semester in college, but I’ve forgotten it all. :(
Awesome
Thank you so muchhhhhhhhh
عظيمة والعظمة لله
Thank you, great lecture!
Please invest in a wearable microphone. Otherwise really good video! Also good with the short selected theory on the left side of the board. Thanks again!
Alternative splicing=gene expression regulation?
Yes, it is one form of regulation of gene expression
you'er the best
Ma'am you look like Pam (Jenna Fischer) from The Office US
are u sure that it is 95% of multi-exonic genes being alternatively spliced? I learned 59%
I got it the number from here: pubmed.ncbi.nlm.nih.gov/18978789-deep-surveying-of-alternative-splicing-complexity-in-the-human-transcriptome-by-high-throughput-sequencing/ It is from 2008, so could be out of date; but I think it is still definitely higher than 59%
A-MAZ-ING
Screw the lecture. Are you single
You are looking alike to my gf ,Indian . 😘
U teaches really well..i am become a huge fan of u..can i get ur email or number mam??..actually i want to shootout some problem related to genomics..thats why i want this.if u dont mind so could u??
You look like emma watson
M'dm u r vry beautiful along with good lecture
R u single?
Do you have a boyfriend?
No, I have a husband.
Biology Professor for how long 🧐?
Quite awhile. We’ve been together over 10 years
@@BiologyProfessor but I want you 🥺