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You are welcome

Could you explain further? Do you mean the curve doesn’t pass through the points?

Thanks for the sub!

Using a logarithmic x-axis (concentration) spreads out the data so the shape of the curve and the quality of the fit are readily visible when the concentrations cover a wide range.

Hello. You can skip normalization of the y-axis values if it is already in the percentage.

Glad you like it!

Hello, It is better to add a small value (0.001 for example) to each value of x before log transformation.

I am glad it helped :)

thanks for your suggestion

Glad it was helpful!

I am glad you like it :)

I am glad you like it :)

Thank you for your question. You can use any units you wish for concentration.

Thanks for your interest in my video. It would be helpful if you could send me snapshots or a prism file so I can better understand the issue and suggest a solution. you can email me at danextreme4jc@gmail.com. cheers

Thanks for your interest in my videos. It would be helpful if you could send me snapshots or a prism file so I can better understand the issue and suggest a solution. you can email me at danextreme4jc@gmail.com. cheers

Thank you so much Dr Gyan. But I was facing the mentioned issues in Graphpad Prism version 6.0 but in version 9.0 the issue resolved by itself. Your video was quite useful for me in determining the IC anything.

@@mariahakim5749 I am glad I could be of help to you.

Thanks for watching my video. The Y-axis represents the absorbance

Thanks for watching my video. I admit that, even though changing 0 to 1 works for some datasets, it does not work for others. A better approach for your case will be to add a small number (0.0001 for example) to all your y-values, run the analysis for IC50 and then subtract that number from the IC50.

Thanks for watching my video. I admit that, even though changing 0 to 1 works for some datasets, it does not work for others. A better approach for your case will be to add a small number (0.0001 for example) to all your y-values, run the analysis for IC50 and then subtract that number from the IC50.

Thanks for watching my video. I admit that, even though changing 0 to 1 works for some datasets, it does not work for others. A better approach for your case will be to add a small number (0.0001 for example) to all your y-values, run the analysis for IC50 and then subtract that number from the IC50.

Hello and thanks for your question. This web page explains what "Ambiguous" in Graphpad prism curve fit mean: www.graphpad.com/guides/prism/latest/curve-fitting/reg_analysischeck_nonlin_ambiguous.htm

Hello and thanks for your question. This web page explains what "unstable" in Graphpad prism curve fits mean: www.graphpad.com/guides/prism/latest/curve-fitting/reg_unstable.htm

Hello thanks for the question. Yes it is applicable to MTT assay

Thanks

Thank you, I will

Happy to help

You're welcome 😊

Thanks. I am glad it helped

Thanks for your question. You just gave me an idea for another video :)

Thanks for your question. Even though you can do the analysis without log transformation, using a logarithmic x-axis (concentration) spreads out the data so the shape of the curve and the quality of the fit are readily visible when the concentrations cover a wide range.

Thanks for the question. You can calculate CC50 with the same method as IC50. The difference between IC50 and CC50 is how you interpret the results

Thanks for your question and sorry for the late response. Which part of the video are you referring to? Is it the part about EC anything? Or IC anything?

@@Dr.DanielAddoGyan Thank you for your response. For IC anything part, around 7th minute of the video, I see that your Y values go from 0 to 100. but Prism says it should go from 100 to 0. It made me confused.

Where did you find the information that says it must go from 100 to 0? Can you share a url if it is from a website?

Yes, you can

thanks for your question. You should normalize if you are sure your lowest y value represents 0% response and highest y value represents 100% response ( the case of agonist. its opposite for antagonist).

I am glad it helped

please can you explain your question further?

@@Dr.DanielAddoGyan can you guide me step by step how to use graphpad prism to analyze Dpph result like plant extract absorbance, methanolic , and ascorbic acid activity absorbance.and which formula we used tell me research paper reference.M new in this field

Well noted. will add references to subsequent videos

Thanks for your question. Do you mean you want to draw a line from the IC50 point on the curve to the Y-axis?

Glad it was helpful!

Did you find the answer? please share

Sorry for very late response. log transformation of X might not be necessary in some cases depending on the nature of the Data and the requirements of the problem. Not sure why you got a bad grade but it will help if you share the question for your course work.

Let me check and get back to you

Do you mean “Can it be performed in prism 9?”

@@Dr.DanielAddoGyan yes

I haven’t tried it on version yet. Could you try it and let me know how it goes :)

@@Dr.DanielAddoGyan There is no SE in EC50 on prism 9

@@chaowang6330 can you follow the steps in the video to find the SE?

You should normalize when your values account for 0% through to 100% response. If this is not the case, there is no need to normalize

I am glad the video was helpful. Your absorbance doesn't have to be exactly like in the video. whatever values you have should work as long as you are using the corresponding concentration values. Also there is nothing wrong with your graph if it falls on the negative side of the graph, the software can still calculate the IC50. If you want to transform the absorbance, you could divide or multiply them by some common factor. Make sure you apply the same transformation to every absorbance value. hope this helps

Glad you like them!

Will do soon

I am glad you like the video. Actually you don't need to convert the OD values to be able to calculate the IC50

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I have not yet tried version 9, so i don’t know whats new though. I would be happy if i could see what exactly you are doing. Screen shots or something would make things easier.

Glad it was helpful!