Visual protocol on DNA cleanup with QIAquick

Поделиться
HTML-код
  • Опубликовано: 29 авг 2024
  • Watch our cleanup video protocol with useful tips and tricks to help improve your downstream results.
    To see our full range of cleanup products please go to www.qiagen.com/cleanup. For further useful tips and tricks in end-point PCR check out: www.qiagen.com/pcrbeginnersguide.
    Please feel free to share this video with friends and colleagues.

Комментарии • 9

  • @thefenerbahcesk4156
    @thefenerbahcesk4156 6 лет назад +5

    I do this shit all the time in lab. The amount of DNA I have decreases by 10 fold from back when I originally prepped the plasmid.

  • @Kayleigh-kx6hb
    @Kayleigh-kx6hb 3 месяца назад

    sometimes there is white precipitate or debris upon adding isopropanol to the gel with the binding buffer. is this precipitate Agarose or the DNA?

  • @lavanyamadhusoodanan3432
    @lavanyamadhusoodanan3432 3 года назад

    If we add proteinase k just the double amount..suppose 40ul instead of 20ul.. to blood during DNA extraction..then what happens?

  • @karliaenidnieveslopez4175
    @karliaenidnieveslopez4175 Год назад

    How much volume should I add to the PE buffer before use?

  • @riffatsultana1100
    @riffatsultana1100 4 года назад

    very informative great

  • @esan120au
    @esan120au 4 года назад +1

    Should have been more user-friendly. Not clear how much sodium acetate should be add if the solution gets orange or violet.

    • @patriciap9211
      @patriciap9211 3 года назад +2

      Their protocol mentions that if the color of the mixture is orange or violet, you should add 10 µl of 3 M sodium acetate, pH 5.0, to the tube
      and mix. The color of the mixture will turn yellow, which means the pH ≤7.5 again (the adsorption of DNA to the QIAquick membrane is efficient only at pH ≤7.5).

  • @mohamedacro521
    @mohamedacro521 8 лет назад

    Wow. Nice work

  • @postmortemarg
    @postmortemarg 5 лет назад +1

    SO many spins