Visual protocol on DNA cleanup with QIAquick
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- Опубликовано: 29 авг 2024
- Watch our cleanup video protocol with useful tips and tricks to help improve your downstream results.
To see our full range of cleanup products please go to www.qiagen.com/cleanup. For further useful tips and tricks in end-point PCR check out: www.qiagen.com/pcrbeginnersguide.
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I do this shit all the time in lab. The amount of DNA I have decreases by 10 fold from back when I originally prepped the plasmid.
sometimes there is white precipitate or debris upon adding isopropanol to the gel with the binding buffer. is this precipitate Agarose or the DNA?
If we add proteinase k just the double amount..suppose 40ul instead of 20ul.. to blood during DNA extraction..then what happens?
How much volume should I add to the PE buffer before use?
very informative great
Should have been more user-friendly. Not clear how much sodium acetate should be add if the solution gets orange or violet.
Their protocol mentions that if the color of the mixture is orange or violet, you should add 10 µl of 3 M sodium acetate, pH 5.0, to the tube
and mix. The color of the mixture will turn yellow, which means the pH ≤7.5 again (the adsorption of DNA to the QIAquick membrane is efficient only at pH ≤7.5).
Wow. Nice work
SO many spins