Immunohistochemistry | How to perform immunohistochemistry? | application of immunohistochemistry
HTML-код
- Опубликовано: 22 авг 2024
- This video explains the concept behind immuno labeling.
What are immunohistochemistry techniques?
What are immunohistochemistry techniques?
What is immunohistochemistry test?
How do you perform immunohistochemistry?
What are the components identified by immunohistochemistry?
What is immunohistochemistry used for?
What does IHC stand for?
this was so much more useful than anything I've learnt at uni! really great visuals and great explanation! THANK YOU
Please share among friends and help me to reach big audience
Dont forget to check out my play
playlists
Immunohistochemistry (IHC) techniques involve the use of antibodies to detect specific proteins or antigens within tissues. The process involves the binding of primary antibodies to the target antigen followed by a secondary antibody conjugated with a detectable marker such as a fluorescent dye or enzyme. This allows for the visualization and localization of the target protein within the tissue sample.
An immunohistochemistry test involves the application of IHC techniques to detect specific proteins or antigens in tissue samples. This can be used to identify specific cell types, diagnose diseases, and monitor therapeutic responses.
To perform immunohistochemistry, tissue samples are first fixed and sectioned onto slides. These slides are then treated to remove endogenous peroxidases and block non-specific binding sites. Primary antibodies specific to the target antigen are then applied to the slides, followed by secondary antibodies conjugated with a detectable marker. The slides are then washed and counterstained before being viewed under a microscope.
The components identified by immunohistochemistry can vary depending on the specific target antigen being studied. This can include proteins, hormones, enzymes, and other biomolecules.
Immunohistochemistry is used for a variety of applications, including the identification of specific cell types, the diagnosis of diseases, the monitoring of therapeutic responses, and the evaluation of research hypotheses.
IHC stands for immunohistochemistry.
❤
I'm training to do this at work, thank you for breaking it down to the basics!
Thanks please share among friends and help me to reach big audience
Today I saw your first video on immunoblotting and after that i covered all my basics from your rest of the videos.. Literally, mind blowing video, content and voice too. Thank you so much... Looking forward for your more videos on microscopy and spectroscopy techniques.
Go to my channel and look at the playlist....everything is there.....
For live classes and doubt clearing session subscribe to my other channel ruclips.net/channel/UC4IpyopsGWSjaPACNTZLuqg
Please share both the channel link with your friends
All my videos are categorized based on subjects in the playlists....so check out my playlist
Excellent synopsis of the concept and process! Thank you very much!
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
Better than professors' explanations, very clear overview! Thank you
please share my channel link with your friends
U helped me a lot!!!
Thank you.
Neurology resident from South India
Please share my channel link with your jouniors
Sure
Very helpful! I shared among my friends.
Please check out my other playlists...it would be useful for you
Your brief and lucid explanations are THE BEST!!!
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
Presented very well and cleared the concept
Please share my channel link with your friends and help me to reach big audiance namaste
This is a super overview! Thanks so much.
Also watch this video for complete overview
ruclips.net/video/Z_SNpo0aW34/видео.html
Please share my channel link with your friends and help me to reach big audience
Very Nice thank you!
Would have been great to have more details about Blocking!
And the different animals used: mouse or goat or rabbit!
But very useful and clear.. Thank you!
You explain everything perfectly ❤️
Please share my channel link with your friends and help me to reach big audience
Thank you so much, very clear and easy understanding !
Please share my channel link with your friends and help me to reach big audience
this is what I needed, just quick, concise, but good explanation. thank you very much :)
If you like my explanation please share my channel link among your friends and help me to reach big audience
Simply put and neat.
Don't forget to revise your concepts using our mcq course ( only @350₹ one time pay and life time access)
Link: animatedbiologywitharpan.graphy.com/courses/CSIR-NET-Life-sciences-MCQ-practice-course-645881d4e4b0c11d957360ef
very well explained thank you very much!!
Monxampion please share my channel link with your friends and help me to reach big audience
Thank you. This was a good summary.
khaled alharmoudi please share among your friends and help me to reach big audience
Explained the topic very well !❤️
Thanks ...please share my channel link with your friends and help me to reach big audience
Thanks, Comrade.
Awesome, Thank you!
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
This explanation is much more immunofluorescent than immunohistochemistry.
Thank you! Good video!
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
U are really good teacher..
__. Ur follower from Jordan
Dania Dabbas glad to hear that.......I so wanted to visit Petra.I was very close this January...I was in Eilat
@@animatedbiologywitharpan
Welcome to Jordan any time
Sir, Thank you for this...
Please share my channel link with your friends and help me to reach big audience
im in highschool ( gr 9 ) trying to act like I understand everything explained here. lol
Lovely explanation.tnx
Please share my channel link with your friends and juniors and help me to reach big audience
what is the difference between Immunohistochemistry and immunofluorescence
Overall they are used in same sense and both use the principles of antigen antibody interaction
But immuhistochemistry is more directed towards tissue
@@animatedbiologywitharpan I thank immuhistochemistry use enzym and immuoflurescence use fluoresent tag
thank you very much
well explained I think you explained Immunocytochemistry instead of immunohistochemistry
The basic Principles are all same , they call it histo chemistry when things are done on tissue and they call it cytochemistry when things are done on cells
Marvellous 💫
plese share among friends
Thank you so much
Please share my channel link with your friends and help me to reach big audience
Nice.. Keep it up
Share among your friends
What are the methods used for signal amplification in IHC?
I have a question regarding colorimetric detection: for the secondary antibody to work, in the cell there must be a process that is regulated by the enzyme attached to the secondary antibody, right? And does this process have to do with the original protein that interests us, the one that is recognized by the primary antibody? Thank you
Great
Please share among your friends and help me to reach big audience
THANK YOU!
If you like my explanation then please share among and help to big audience
What is diffrence between IHC and elisa and the best techenique to be used to determine enzyme presence
Elisa would be the best technique and it’s more sensitive
@@animatedbiologywitharpan thank you so much
Legend! Thanks for your video :)
I am just a simple person who is sharing concepts with friends
Please share among your friends and help me to reach big audience
Bhai biotechnology or biological science k liye same study plan Kam karega ya nahi
I think yes
Nice
Please share among your friends
Please do not confuse between immunofluorescence and immunohistochemistry? What happened to antigen retrieval? this video can misguide people
Exactly what I was thinking. He kept mixing up the steps between IF and IHC. IHC needs antigen retrieval step done with a buffer and exposed to heat and pressure, not triton or other detergent like you mentioned for permeabilization which is done for IF.
Why are doing secondary ab incubation in dark?
Secondary antibody is generally coupled with fluorophore....if you dont keep in the dark then it would bleach
Williams Sandra Rodriguez William Taylor Scott
nice video
Please share among your friends and help me to reach big audience
Can u say me the correct strategy preparation (which topics to cover)to clear IIT JAM with a month ?
ruclips.net/video/wTscLY2NNyk/видео.html
ruclips.net/video/cWf_NkqvjRA/видео.html
i like your video, but I think this is not a Immunohistochemistry, this is a mmunofluorescence tecnique.
These terms are really subjective , when you use enzyme based dete tion it's histochemistry, when you use fluorescence based detection it's immunofluoresence...but the principle is same.
is fluorescent an enzyme?
No no no. Fluorescence is a molecular phenomenon
Fluorescence is a process where a substance absorbs light at one wavelength and re-emits it at a longer wavelength. Common in dyes and certain materials, this phenomenon is widely used in biological imaging, chemical analysis, and even in everyday items like highlighters, showcasing its diverse applications in science and technology.
ahh, i see. now i can answer the quiz from my professor about this. thank you so much! @@animatedbiologywitharpan
Huge mistake..please before u upload the video cross check..Epitope is a part of the antigen..not antibody..part of antibody name is paratope..
Ravi chandran please watch the video carefully.......
U listen time 1:30..what u told a epitope part of the protein by antibody..which means
Ravi chandran ya epitope is a part of protein ......so what is wrong about that my friend ....antigen is also protein most of the cases.....you are missing out something
Epitope is part of protein I agree..ne sonadhuku meaning எபிடோப் என்பது ஆன்டிபாடியால் புரதத்தின் ஒரு பகுதியாகும்
Thank you very much, sir.
Please share my channel link with your friends and juniors