Simulating Restriction And Insertion Cloning In SnapGene

In this tutorial, I will show you how to simulate conventional restriction and insertion cloning in SnapGene. Restriction cloning is a common cloning technique where restriction enzymes are used to prepare an insert and a vector for ligation. I will explore the ways to simulate inserting a single fragment into a plasmid vector using restriction cloning in SnapGene.
A BEGINNER'S GUIDE TO SNAPGENE (ONLINE COURSE)
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[@StevenBradburn]

A BEGINNER'S GUIDE TO SNAPGENE (ONLINE COURSE)
courses.toptipbio.com/p/beginners-guide-to-snapgene

[@Jyoti_B06]

Thank you so much for the video. I was finding it difficult to use. Make more videos😁😁❤

[@guihuajia7696]

very great and clear tutorial!

[@indiraprakoso5439]

Thank you so much

[@kashafjabeen802]

I follow the same steps that are given in video but the sequence of my gene of interest in cloned vector (product) is in opposite direction. please guide me Sir.

[@pitbolibo]

what if the the second plasmid doesnt have the same restriction enzymes??????? forgot that

[@dencelestra506]

HI! I know it has been a long time. I just would like to ask if it is okay if my gene of interest has a higher kb compared to my original vector... my GOI contains roughly 30kb while my original vector only contains ~5kb.

[@georgenaugles5039]

The beginner's guide to Snapgene pUC19 plasmid seems different from the addgene pUC19 plasmid, with different 0 base pair indexing, making it a challenge to achieve the same or similar view of the pUC19 plasmid in the actual SnapGene to match the instructional video...may want to either make them the same or give more instructions about how to change the indexing of features.

[@StevenBradburn]

So I believe I imported these plasmid files into SnapGene through the SnapGene Online Sequences option, rather than the AddGene Plasmids option. So this may explain the differences.
If you would like to change the origin/numbers of the plasmid, go to View>Set Origin. You can also flip the sequence by going to View>Flip Sequence.
For example, for the AddGene version of pUC19, you can flip the sequence (View>Flip Sequence) then change the origin (View>Set Origin, use a value of 2000) and this should resemble the default version from the SnapGene database.
I hope that helps?

[@abstractss2053]

Hello, can you tell me for what reson we use this method in lab? , what is the practical use of it?.

[@francescosilvestro2092]

By simulating RE digesting or displaying sequence you can PERFORM knockout experiment, insert gene of interest. Any kind of application of recombinantDNA. Inserting sequence to build up crispr-cas cassette. Any GENETIC manipulation, in other words.

[@Pardesizindagi88]

I can't see any "insert option" after I added vector instead of insert option there is "fragment" between vector and product whyyyy??

[@Farhab]

Fragment means gene of interest.