CytoSMART Multi Lux3 FL - Simultaneous fluorescence live-cell imaging

Fluorescent imaging with the CytoSMART Multi Lux3 FL enables distinction of cell types in co-cultures when each cell type is assigned a specific fluorescent label. This can elucidate interactions between cell types, which can be relevant for e.g. tumor growth. This video displays four co-cultures of tGFP-labeled HeLa cells (green fluorescent) with non-fluorescent 3T3 cells, at various seeding ratios. These cultures were monitored during 90 h with a 1 h imaging interval using the CytoSMART Multi Lux3 FL. Confluence per
cell type was determined from confluence per imaging channel in the CytoSMART cloud. The confluence per cell type revealed competition between the cell lines, with the HeLa cells eliminating the 3T3 cells.