How to Make Agar Nutrient Media for Orchid Seeds - Quick and Easy Process
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- Опубликовано: 9 авг 2021
- In today's episode, I'm sharing how to make the agar media for growing orchid seeds. I'll share my process on how to create the nutrient solution for sowing orchid seeds. This is the germination media used in aseptic orchid seed sowing. It's the first step of several to grow orchids from seed. There are many ways to do this and many variations as well...this is what I do and what works for me. I'll also talk about the reasoning behind this procedure and why I do what I do.
I'm using Phytotech P723 Orchid Sowing Media for epiphytic orchids. Terrestrial orchids require a different recipe for agar media. For sterilization, I steamed the solution for 40 minutes. If you were to autoclave it or use a pressure cooker (121C at 15psi) with the same volume (50ml), then it needs about 15-20 minutes.
References:
- Here but Not: • Growing Orchids From S...
- The Orchid Room: • Unboxing and Flasking ...
- Orchidomania: • Video
- Growing Orchids from Seed (book) by Philip Seaton and Margaret Ramsay
- A Guidebook in Orchid Micropropagation: issuu.com/marcoacuna/docs/a_g...
- Facebook Group: Orchid Flasking / Orchid Seed Sowing and Seed Exchange
Music: "Freedom" by Atch
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Growing Environment
Orange County, Southern California (USA)
Zone 10B: Subtropical/Hot-semi arid climate - hot, dry summers / mild winters with low to moderate rainfall
Average Summer Outdoor Temperatures: 63-83F
Average Winter Outdoor Temperatures: 48-70F
Average Rainfall: 13 inches/year
Indoor Temperatures: 65-85F
Indoor Humidity 40-60%
East/West facing windows
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THINGS I USE:
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- pH Meter: amzn.to/3dq5XS9
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- Plant Moisture Sensor: amzn.to/370Bb16
CAMERA GEAR:
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DISCLAIMER: Links included in this description may be affiliate links. If you purchase a product with the links provided I may receive a small commission. Thank you for supporting Attainable Green so I can continue to make awesome content for you to enjoy! #orchids #orchidseeds #agarmedia
Excellent Tutorial Jess ! You explain everything in Layman's terms... :)
Thanks! I wanted to keep it simple to show that it’s not hard to do. 😁
Very cool and exciting! Looking forward to seeing your updates on this project.
Thanks! I’ll have a video out tomorrow about flasking the seeds 😁
Thank you for taking your time sharing your knowledge. 🙏 Very interesting information!
You’re welcome! Glad you’re enjoying the videos! 😁
Amazing! Thank you for the helpful information 😊
Thank you for your great information.
Excellent video. Thanks for the tips. 👍👏
Thanks for the update 😊🙏 really excited about where this is going. Great explanation and really good tips. Fully agree with you on starting with the basics and getting that right. The commercial guys have it so easy with their laminar flow and autoclaves 😅. I keep on reminding myself that these techniques were around way before any of the fancy equipment.
Haha yea! Fancy equipment definitely helps speed up the task, but having good technique is better still. A lot can be done with simple items, so I don’t worry about having the “right” things. 😁
What an amazing video!
Thank you! 😁
Excellent information -- I may give starting orchids from seed a try!
Yea! They’re definitely worth trying and there’s lots of different techniques and ways to go about it. If you’re not successful with one method, there’s many more to test out. 😁
Very interesting! 🐈😍
Thanks! 😁
Nicely done 💝
Thanks! 😁
Very interesting info. I do not intend to do it but I still like to know how it is done and so I appreciate your vídeo 🙏
Yea no problem! I don’t think it’s for everyone but it’s cool to see how it’s done 😁
Hey I know I am kinda late to the party but I really need to know if its possible to just boil/cook the jars/flasks? I dont have a pressure cooker but I have managed to polinate a orchid of mine and really want to continue the work.
I don't have this ,can I use ager from the supermarket.
Hello im looking for media whar can buy in Europa?
Where to buy the powder mix?
Where can you get this media?
Thank you..
Can I use ordinary nutrient agar instead of this agar...
Do u think it wld work?
How to contact you... where could i get the phyto tech sowing medium ?
Any success/update? I want to try just as a hobbyist as well. Thanks!
There may be a playlist that has some updates on these flasks. So far, I have about 5 flasks growing well and the rest are still in experimental phases. I'll put up a new update in early 2023. Thanks for stopping by!
Where do you buy Orchid seeds-phytotech p723? Thank you
Hi, Jess thank you for your video about How to Make Agar... Please advise me on where I can purchase Germination media P723? Thank you,
How do l get p723media
I had tried this before but i fail. But its my fault not sterile properly it gets moldy since then i didnt try again. This instant version maybe ill consider.
Mam how to order agar P723
Hey Jess! Thanks for making this video. I've been watching some mushroom cultivation videos and I wonder if I can use mason jars with self-healing injection ports to cut my risk of contamination? Let me know what you think! I think I can instantpot-sterilize my agar solution in a self-healing mason jar, then inject the dry seed + peroxide solution via the self-healing port. Do you know which gauge needles would be large enough for orchid seed?
It might work, I haven’t quite considered it. Some orchid seeds are small, about the size of mushroom spores so those might work. For larger seeds like from complex Dendrobiums or other types, they might be too large for a typical syringe. I had trouble getting some seed out with a plastic 2ml syringe with no needle tip…really just depends on the size of the seed
The best way to cut on contamination is to use green seedpods, they come sterile from nature. I flame a lancet, divide the seed-pot and use a sterile tool to pour few seeds in the flask.
Peroxide, syringes, Clorox... are noise in the system if you have access to a laminar flow hood.
😂 was thinking the same thing
Hi, may I know if P723 is good for Phalaenopsis? Thank you.
Yup! P723 can be used for phals. 😀
Where did you by the agar mix, was it online and what is the link?
I got it from Phytotech Labs in USA: phytotechlab.com/orchid-seed-sowing-medium-p723.html
Hello friend I needed blue berry seeds jarminetion midiya,
You help me frends
after you planted the seeds whereby you incubate the jar? and for long?
After seeding the jars, I leave it alone in a slightly lighted location. It took about 3 weeks for strong germination and it still continues to grow in flask. I am waiting for the first leaves and roots to grow before I think about reflasking into a larger space. I believe the plants stay in flask anywhere from 1-2 years.
If you are doing solid media, there is a germination media and a replate media. The germination depends on the species of orchid. Some take a few weeks, some take much longer. The species and the medium you are using makes the difference. I like to move my protocorns to my replated media and let them to growth there. Sometimes you have to replate your replate if all your protocorns made it to orchids.
Where did you buy Phytotech P723 ?
From the Phytotech website.
I sometimes get plants (seedlings) that could need some more tlc would agar be a good idea to grow them on ?
If the plants are small enough, it may benefit from a little more time in agar. It would need more nutrients than the P723 media since that is usually used to germinate seeds. P748 or something similar (replate or maintenance media) would be better since it has more nutrients to sustain a larger plant.
@@AttainableGreen I will give it a go. Searching for the media I found a website that shows step by step what you did in your video including building a flowhood to keep it all sterile really interesting and how to propagate from nodes as well 😁
Super cool! 😁
How can I make my own powder medium we materials that are easy accessible
I have not made my own agar powder medium so I cannot vouch for the efficacy of homemade media. There’s recipes in this link that have been tested and used in lab settings: issuu.com/marcoacuna/docs/a_guidebook_in_orchid_micropropagat/30
@@AttainableGreen hi do u have a recipe that requires ingredients that is accessible for growing orchid seeds plz
You can search peer review papers. There are some specially in Brazil and Peru using krystalon orange fertilizer, sugar and cooking agar and seems to work fine.
O kullandığınız siyah toz veya sıvı nedir
Kömür ve diğer besin maddeleri içeren agar agardır.
Diğer besin maddeleri nelerdir
Bu web sitesindeki besin listesi: phytotechlab.com/orchid-seed-sowing-medium-p723.html
You lost me a powdered media? what is that?
Öncelikle teşekkürler ederim bi süredir araştırma yapıyorum, sizin sayenizde öğreniyorum😊 kırgızistan Kırgız kızı yım türkiye de yaşıyorum İngilizce öğrenmeye çalışıyorum sizden ricam Turkçe alt yazı bazı yayınlarızda yok lütfen sizden ricam Turkçe alt yazı oluşursa sevinirim sevgili saygıyla goruşmek üzeri 👋
Hi sis i am nepal i want orcid farming nepal can u help me patnar bignes
Why don’t u just tell us the ‘powdered solution ‘ name?
1:37
The media leaf it out side for 3,day
The media is put away for 3 days to check for contamination. If it does not grow mold or bacteria, it’s good to use. Not for outside.
I couldnt follow the end because her voice is extremely irritating!
Aww boohoo