Phase contrast microscopy || Principle | Mechanism | Advantages and disadvantages

#micriscopy
Phase-contrast microscopy is an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image. Phase shifts themselves are invisible, but become visible when shown as brightness variations.
When light waves travel through a medium other than vacuum, interaction with the medium causes the wave amplitude and phase to change in a manner dependent on properties of the medium. Changes in amplitude (brightness) arise from the scattering and absorption of light, which is often wavelength-dependent and may give rise to colors. Photographic equipment and the human eye are only sensitive to amplitude variations. Without special arrangements, phase changes are therefore invisible. Yet, phase changes often carry important information.
Phase-contrast microscopy is particularly important in biology. It reveals many cellular structures that are not visible with a simpler bright-field microscope, as exemplified in the figure. These structures were made visible to earlier microscopists by staining, but this required additional preparation and thus killing the cells. The phase-contrast microscope made it possible for biologists to study living cells and how they proliferate through cell division. It is one of the few methods available to quantify cellular structure and components that does not use fluorescence. [1] After its invention in the early 1930s,[2] phase-contrast microscopy proved to be such an advancement in microscopy that its inventor Frits Zernike was awarded the Nobel Prize in Physics in 1953.
Bright field microscopy:-
• Bright Field Microscop...
Dark field microscopy :-
• Dark field microscopy ...
Fluorescence microscopy:-
• fluorescence microscop...
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[@Anu-me7hv]

Very helpful..... can't find such a simple video.....lots of thanks ❤🙏

[@UGCSManikandan]

This helps me to understand easily how this microscope works😍 really awesome n clear explanation 😍😊👌

[@hritikseth9465]

This is Just the Mechanism of. any just Random Microscope even Bright Field... Interferences aided by Annular Rings and Phase Rings Made Phase Contrast Possible which is not included in Video.. Please Mention Interference Happening as its the Crux of tje Phase Contrast Microscope

[@dharavala6144]

Vry nicely nd smoothly explained great job thank you sir

[@muruganlogesh9331]

This video helps me to understand

[@Madhunisasartandcraft14]

Thank u

[@s.abirami8394]

Bro well done you just made it crystal clear without dragging the lecture 🙂

[@bioscience6853]

Thank you

[@himanshibakshi7304]

Thank you. It was very helpful and easier to understand.💯

[@sanjaymortha0426]

Thank you sir.....good simple presentation

[@bioscience6853]

☺☺

[@darshanadeore3145]

Very good and easier presentation sir.. Thank you..

[@bioscience6853]

☺☺

[@sowmiyasiva3569]

Super bro keep doing more videos

[@bioscience6853]

Thank you and sure

[@shraddhacutie4236]

Thnku so much sir loved all ur videos 🌺🙏

[@pruba7009]

Thank you dear Alampally. I couldn't understand why the exact measurement of specimen is not possible?

[@ipsvipulsingh9110]

Gr8

[@bioscience6853]

🙂🙂

[@vijjumahankali4297]

Thank you so much sir 👍👍👍

[@bioscience6853]

My pleasure

[@dreamer5911]

Thank you sir

[@bioscience6853]

My pleasure

[@theaneezeh4614]

Tysm was so good

[@bioscience6853]

My pleasure

[@preetysuman3719]

Sir I want to ask you that what is the advancement in light microscopy?

[@Ram-ky7ez]

We are not in 8th standard bro please explain it in details.

[@bioscience6853]

I too didn't mentioned as detail explanation brother.. It's just a basic info

[@Kate-hu9be]

Guess: onion root tip cells🤔

[@ashfaqpeerzade166]

Mitosis : telophase , anaphase and prophase

[@ashfaqpeerzade166]

Last one is it right

[@jennylynganiban3164]

urine crystals?

[@fathimaafrin1135]

Mitotic stages of cells

[@suyanksrivastava8235]

Sir i am not join your whatsApp group these massage show whatsApp group are full

[@suyanksrivastava8235]

I want to join whatsApp group

[@abhijithdd2458]

0:54 1:00

[@easurajaneasurajan3855]

Zernike (1935)

[@sudipchatterjeerobonik3379]

its skin cells i guess

[@priyangajagadeesh7127]

Ice cubes

[@veghajirathod5760]

Hindi me bol na bhai