DIC Microscopy | Differential interference contrast | DIC vs Phase contrast.

DIC is also termed as Differential interference contrast Microscope. This microscopy technique uses the polarizer and a special type of prism to split light into two rays (or two waves) known as the e ray and the o ray. Both the rays are separated by a small distance known as the shear distance. There is also a phase difference between both the rays. Both the rays passes through the specimen and further hits the second prism which combines them into a single ray. finally the image observed has one bright region and other dark region. the physics involved in DIC microscope has been discussed in detail. Finally we also compare DIC and phase contrast microscope as both of them can be confusing.

[@asphyx9]

Thank you - one of the best explanations I have seen on DIC

[@yixuanshen8366]

Dude, you are saving my life

[@tarunkumarpalli7580]

Wow dude, its so, clear to understand

[@nourelhoudabenyahia257]

Amazing explanation!

[@phamdung3884]

This really is a simple explanation. Thank you so much! ^^

[@potharlaanil5621]

I salute for your explanation..

[@peterallen8573]

Good explanation.Thank you.

[@moneeralhalabi1272]

Great work

[@keshaxLOVEx]

very useful thank you

[@anindita6913]

Excellent

[@shivanin8708]

Thank you so much!!

[@christeenamathew8606]

crystal clear 🤟

[@CHAMPIONSBio]

Thanks a lot bro .....

[@nihalhathaway4089]

thank you very much! Isn't the condenser after the polarizer, between prism and stage?

[@mortentorres3758]

yes it is. you are right

[@pak2966]

Are the two Nomarski prisms exactly the same (just inversed) or do they differ?

[@gregvisioninfosoft]

Does anyone here have broad experience using DIC? I was hoping I could ask a question. I am trying to understand this in a general manner. From this video at 0:06 timestamp, we see that two (I assume 'matched') 'DIC Nomarski Prisms' are required. One below the sample, and one above. Apparently there is no concept of a single one-size-fits-all 'DIC Nomarski Prism. As when I look at actual part numbers, for example in the Olympus microscope line, there are several of these: U-DIC-10, U-DIC-20, U-DIC-40, U-DIC60, and U-DIC100. What I notice in the differing catalog numbers is what appears to be the 'Magnification' is different. This implies the DIC Nomarski Prism is somehow 'optimized' to a given magnification. My first question is - what actually causes these DIC Nomarski Prisms to be different in their manufactured characteristics? In other words, if one were to hold both a U-DIC10 and compare it to a U-DIC20 - how exactly would they be different?

[@user-yj6ye4zk8v]

5:34

[@JongcheolLee]

Wow best video ever explaining DIC

[@mototajo8911]

Thanda

[@user-yj6ye4zk8v]

6:16