Upgrading a Cheap Microscope Lets You See Rainbows! - Polarized Light Mod
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- Опубликовано: 6 июн 2024
- Normally the ability to do polarized light microscopy at least doubles the price tag of any new microscope you purchase. And that's kind've of a shame because with only the addition of 2 simple polarizers a whole new world of detail is opened up.
This mod requires only the purchase of 2 cheap pieces of polarizing film and everything takes only a few minutes to set up. Once done, the effect can be turned on and off at will. See the stresses inside fibers or defects in glass as crazy color changes or spot minerals that were otherwise invisible.
Dark field microscopy is also interesting as it uses a simple optical hack to give the impression of a dark background for subjects. The conversion process is just as easy as polarized light and can also be set up in a couple minutes.
Focus stacking- • Focus Stacking: The Se...
The lichen responsible for modern biology - • The Experiment That Di...
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![TRASH or PASS! Eminem ( Houdini ) [REACTION!!!]](http://i.ytimg.com/vi/cB1WrZcMwMc/mqdefault.jpg)
Love your channel!! Pls don't stop, you're the only one doing quality DIY videos on biotech!
Thanks :)
LOL, era improvável, mas te encontrei aleatoriamente no youtube hahaha
Every video keeps getting better! You have a talent for expressing your passion for science through these videos and that's what makes it so interesting for viewers with similar interests. Keep up the great work!
thanks :D
Awesome Video , after watching this video I did the polarized modification and the results were amazing. Only thing i did differently was that I purchased a a cheap pair of polarized glasses and cut the lenses out using a bandsaw. Can't wait for future videos !!!!
Thank you!!! I've just bought a microscope two weeks back and this is just what i need. Thanks a lot :)
Glad you enjoyed :)
Be careful using aluminum foil as a darkfield stop; it can reflect heat back into the lamp and cause it to overheat (less of a problem for LED scopes). I've used black cardstock which works well and absorbs a lot more of the light rather than bouncing it back downward
Yes, it's great to see these cheap and easy ways of producing more advanced modes of illumination. I have found that you can get really good darkfield images by leaving your microscope light switched off and just shining a reading light or even a strong torch (flashlight in American) across the axis of the normal brightfield lighting, ie horizontally, parallel to the floor. It's a pity there just doesn't seem to be an easy do-it-yourself way of getting phase contrast lighting, unless you have invented one!
Man, I'm subscribed in at least 100 Channels but the best of them it's yours, no dulbt...
thanks :D
Cool. Feel like you've seen my Amazon shopping cart hold list. LOL.
If I ever do get the microscope I'll definitely try out both mods.
Cool video, easy to understand. Thanks!
This was probably the coolest part of my aerospace structures lab. We used the same concept to visualize stress concentrations around a hole in a material. The material itself in our case was one of the polarizers but it could be applied to any material that has a noticeable birefrigence optical property.
Neat! Cool concept
Thanks a lot for this excellent tutorial! I can confirm this works very well, but the polarizing filters need to have NO scratch, else the image will be very blurry! I found that polarizing sheets can often come scratched, whereas polarizing glass filters for cameras are more robust and provide the same result (that's a tip I found on a microscopy forum), so in the end I am using polarizing filters for cameras and this works very well. Also, using 2 circular polarizing filters is better than the typical linear polarizers because with circular filters we can still see some material that would go totally extinct with linear, as described in this paper: Higgins, M. D. (2010). Imaging birefringent minerals without extinction using circularly polarized light. The Canadian Mineralogist, 48(1), 231-235.
I'm not really that interested in biology, but the colorful images look amazing. Thank you for making this video.
Thank you so much. Very helpful.
As an alternative to darkfield you could use colour filter discs and a coloured annular to try Rheinberg illumination.
Awesome! Thanks so much!
Not a nematode expert, but by the way that worm moved, I think its a microscopic annelid. All the nematodes I've looked at move by thrashing about, whereas your worm is using peristalisis.
Huh good point, you're probably right. Nice catch!
Well it also looks segmented, and nematodes aren't segmented afaik. It doesn't look like any nematodes I'd seen when I worked as a marine ecology assistant one summer in highschool.
You are the best thank you for the useful video, I actually enjoyed it and I'm about to polarize my Microscope. thx
This is awesome. I hope to be able to use this to do petrography at home with a cheaper microscope.
It is surprisingly underwhelming how underrated your channel is
Despite the high quality
You can also use the lenses from 3D glasses used at your local theatre.
Awesome, thanks!
Very good insight of polarized and of dark field microscopy. I'm curious about a couple of things, though. As my degree is in photo science, with a good splattering of basic optical design and engineering:
1. Do you really need to stack the black mask or is that to create opacity for the mask. Why can't a singular, opaque circle of the correct diameter, mounted onto thin plastic be used?
2. Why does the positioning of the upper polarizer need to be so critically placed (on the inside, upper housing v/s the bottom of the eyepiece?
Thanks, great work and narration.
Dude I honestly believe you are so underated, you should have at least few hundred thousand subscribers.
I will perform both of these mods; it is so AWESOME :D
Thanks :)
With a Trinocular, Polarisationfilters work also at the 0° Eyepiece, the one on the top, i would guess because the light dosn't really get disturbed that way.
I have some old IMax 3d glasses wondering if I can uses the len's. This looks cool
Can I use polarizing filters that you put on the lens of your camera?
Geologists, at least at my university, have to take several courses in rock/mineral ID using polarized & cross-polarized light. It's called petrography.
You deserve more subscribers
a laymans way to do the first lart is to just get one polarisaer and your phone, or any lcd screen. place the object between your screen and the lens, and show a white screen on max brightness. rotate either the screen or the lens, and enjoy the pretty colours
You deserve more credit
I'm wanting to mod the OMAX microscopes to use a better color LED light. Do you think that looks easy to do with this scope?
Yea, two Polarizers are fun, but throw in a third one into the path and give 'em all
a twist to blow your mind! Try circular Polarizers. Movie theatre 3d glasses are fine.
Waves move through the Aether _and there's not a single particle in sight..._ lol
FindLiberty a third one? what do you mean?
Did you used to work for Acendance Biomedical?
You have good knowledge of optics
Thanks :)
Would this work with reflected light microscopy?
If you put a piece of polystyrene (e.g. smashed petri dish lid) between the first polarizer and specimen you will get a retardation plate. This creates some absolutely stunning background effects like colour gradients and such.
How easy is to upgrade it to be phase contrast microscope ? I would like to observe live cultures of bacteria with Omax microscope
One thing I saw in a research paper was dark field microscopy to see Noble nanopartícles in a Silicon substrate. Do you know if we can do the same using your technique? Your "homemade" dark microscope. Ty!
Hmm maybe. I'm unfamiliar with the technique so can't say for sure
What is the difference between polarized and cross-polarized light microscopy to cause larval otoliths to become binfrigent.. would this work?
is it possible to use a circular polarisers ?
Is it possible to build Schlieren effect on to a microscope to see gradients of density maybe combining it with the Polarisation mod?
Should be right? That'd be dope
Quick tip: 3d glasses from the movies are a great source of polarizers
Hi bro, that is not a nematode it is an anelido, from the Polychaeta class, it was a nice video to
(1:50) It's called _birefringence,_ not "bifringence".
I identify as transfringent
I want to replace the very hot lightbulb source with some LED, (using a pi cam) I tried a white LED and a 365nm LED. What would you suggest? Perhaps a RGB ?
I'd stick to white honestly. You can do rgb but you'll end up with weird effects.
I understand that the mounting of the RGB could be problematic to obtain a constant color mix…, if it is that what you're referring as "weird effects" ?
But you can buy color filters for "white light" microscopes...,
That's my reason asking about RGB.
Could also add UV and IR leds so that the RasPi could change the colors automatically while taking pictures.
Could be a nice Open Source project, but I can understand that if you already have white "LEDs", their is no reason to change… but if you have a Pi cam mounted…
Perhaps getting other viewers, while YOU explain to us "How UV fluorescence microscopy works" (and caveats), doesn't have to be a long video.
It's absolutely staggering that you don't have more subscribers.
Best way to fix that is share the video. I'm kind've a slave to the youtube AI right now. If it decides to share my content the channel does well. When it doesn't things stagnate no matter how many videos I make. It's sort've unfortunate but is the way it is. The channel will grow eventually I hope.
I shared your video about lactose intolerance because I thought it was some really unbelievable stuff but good to know. I can appreciate that the lack of advertising on your channel creates a negative-bias towards the RUclips AI to share your videos but good luck man.
What are polarizes
Is it possible to do phase contrast with a hack like this?
Should be. It works similar to dark field images, so you'd use similar things
Maybe you want to try to make microlattice?
One of my goals is to get into quantum physics since it’s so unexplored but without college degrees how do I go about getting into that type of of field
You basically don't. Quantum physics, at least experimentally requires huge funding to explore any of the few knooks that are left to sus out. So getting a degree and becoming part of a research project is how to get involved
@@thethoughtemporium I think I’ve just got a lot of just learning in general and thanks for the info
The graphic at 1:23 is wrong. Compare the orientation of the light passing through the filter to the orientation of the filter.
Ok, 30 seconds in, and that's insane for 150$. Wtf the future is now.
Cant you use two polarisers to vary the brightness of light underneath as opposed to the aluminium
It'd be easier to just turn down the brightness at that point, so not really
The Thought Emporium you .may not have a variable light supply ..
DIY microscope lens with sugar (or something else) crystal
I just watched you on vice. Its cool you guys got some notice. Hopefully someone out there sees it who is willing to invest with your team. That Aaron dude is such a douche. Hope all your equipment was okay. Thats a lot of money just thrown to the sidewalk. Does your team have a new space to continue reaearch?
Also you can do darkfield and polarized at the same time however you must use/make glass darkfield filter since almost all plastics polarize light. Check out my channel yo find out how to do that
3:27 partial Romanian flag spotted bottom right
Good Vid..and is it snagglepuss talking.
Same microscopes in my school lol
so this is what not wasting your 20s in video games looks like, impressive
Use your 3D glasses from the cinema, if you want to experiment but not buy anything.
Not an area I know enough about, but I believe cinema 3D uses circular polarization. I wonder what impact that would have on the results of this project.
Joats yes they do, but the glasses are analysing filters which make the circular polarization to an linear. Left and rigth hand polarized ligth produces linear polarized ligth with a 90 ° rotation. A second linear pol filter seperates them per eye.
If you shine ligth thru the glasses they get linear polarized, is what i wanted to say XD
I know there was a reason why I kept them :D
+Dute Nait, Thanks for the reply, you gave me the kick I needed to at least read the wiki article on Polarizers (and so I end the day slightly better informed than I started it, cheers).
some Old or broken laptop /pc monitors also have polarising screen.
The funny thing is that is the LITERAL EXACT microscope that I am using.
What model is it? I can't tell looking on the website
How can I see my sperm with my $20 microscope? 😂😂😂
btw pls tag me
Thank You... lol I am a 'Home Geek Wierdo' accprdoomg to my friends .... this new interest of mine would fried them out lol My best closest mate calls my "Spooky at a Distance" already, because I Love PhotonLightTherapy.... I'm a singer/Songwriter ( award winning amongst peers} so you can imagine why 'They' think I am weird - guess whose doing the looking/thinking.... I just consider myself as Switched-On .... This world is Fascinating, and when I feel down it's these things that switch me On again.... accidentally found your Chanel because I want to Program Water - we know that can be done, and use slivers of 'Programming Gems and Chrystals" I also want to see it works, or not - figured this was the only way I could know for sure, and keep the Quality of bounce-to-light-to-Human-Health consistent..... and the Dark-Matter stuff seemingly surrounding everything has captured my interest too, as I imagine it's an Active Component around/through everything , which we don't really understand its activity in all/through all things yet"|? see, my Weird Science that I just call "Thought"Thanks again