How To Filter-Sterilize Aqueous Solutions using Steripak™-GP Filter Devices

This video shows how to filter tissue culture media using Steripak™-GP filters. Watch our Millipore® scientists to learn how to use these filters with a vacuum- or pressure-driven systems such as pressure vessels or peristaltic pumps. With this step-by-step video you will learn how to:
• Filter and sterilize volumes of tissue culture media up to 10 or 20 L
• Set up a pressure vessel for filtration use
• Use a peristaltic pump, including how to adjust flow rates for optimal filtration and sterilization
• Use aseptic technique to avoid contamination during filtration
• Apply the Steripak™-GP filter, and discard it properly
Millipore® Steripak™-GP filters are designed for pressure-driven, large scale sterilization and filtration of tissue culture media, with or without serum. Key features include:
• Millipore Express® PLUS PES 0.22 µm pore membrane for fastest flow with low protein binding
• Larger scale, pressure-driven filtration device for faster flow rates reduces the time needed for large-volume filtrations
• Manually-operated top air vent to prevent interruption of flow due to air lock
• Filling bell for cleaner filling environment around the opening of the receptacle bottle
Explore how these filters can be used in buffer preparation, media preparation, and the sterilization of aqueous solutions.
For more information on large scale filtration of tissue culture media, please visit: ms.spr.ly/6055w...